Cbfa2t2 (NM_001285446) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A430091M07; C330013D05Rik; Cbfa2t2h; MTGR1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227369 representing NM_001285446 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGTTCATGGAAATGGAAAGAGGCCCAGTCCGGAAAGGAGGGACGAGAATAATTTTGAAAGAGATA CAGTTCCTCCTGAACCGCCTGCCAAGAGAGTGTGTACTATCAGCCCTGCTCCTCGGCACAGTCCCGCCCT CACTGTTCCCCTTATGAATCCTGGGGGCCAGTTCCATCCTACTCCACCACCTCTTCAGCACTATACACTA GAAGACATTGCAACTTCTCACCTGTATCGGGAACCAAACAAGATGCTAGAACACCGAGAAGTTCGTGAGA GGCACCACAATCTTAGTCTCAATGGAGGCTATCAAGATGAGTTGGTAGACCACCGTTTGACAGAAAGGGA GTGGGCTGATGAATGGAAACACCTTGACCATGCACTGAATTGCATTATGGAAATGGTAGAGAAAACTAGG CGCTCCATGGCAGTTCTGAGGCGCTGTCAGGAATCTGACCGTGAAGAACTCAACTATTGGAAACGGCGGT TTAATGAAAATACAGAGTTGAGGAAAACTGGGACTGAGTTGGTCTCTAGGCAGCACAGCCCTGGGAGTAC AGATTCTCTCAGCAACGATTCTCAGCGGGAATTTACCAGTAGACCAGCAACAGGATATGTGCCTGTGGAG TTTTGGAAGAAAACAGAAGAAGCCGTGAATAAGGTGAAAATTCAGGCCATGTCAGAGGTACAGAAGGCTG TAGCTGAGGCAGAGCAAAAAGCCTTTGAAGTGATTGCAACGGAGAGAGCTCGAATGGAGCAGACCATAGC GGATGTCAAACGGCAGGCTGCAGAAGATGCTTTCCTGGTCATCAATGAGCAAGAAGAGTCCACAGAGAAC TGCTGGAACTGTGGCCGAAAAGCCAGTGAGACGTGCAGTGGCTGCAACATTGCTCGCTACTGCGGCTCCT TCTGCCAGCATAAGGACTGGGAGCGGCACCACCGCCTCTGTGGCCAGAGTCTACATGGCCACAGTCCCCA CAGCCAGAGCAGGCCACTGCTCCCTGGAGGGCGGGGCTCAGCCAGGTCTGCTGACTGCAGTGTGCCCAGC CCAGCCCTGGACAAGACCTCAGCTACCACCTCACGGTCCTCAACACCTGCCTCTGTGACAGCCATTGATG CCAACGGGCTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285446
Insert Size	1134 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285446.1, NP_001272375.1
RefSeq Size	6076 bp
RefSeq ORF	1134 bp
Locus ID	12396
Gene Summary	Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes. Via association with PRDM14 is involved in regulation of embryonic stem cell (ESC) pluripotency. Involved in primordial germ cell (PCG) formation (PubMed:27281218). Stabilizes PRDM14 and OCT4 on chromatin in a homooligomerization-dependent mannerCan repress the expression of MMP7 in a ZBTB33-dependent manner (By similarity). Through heteromerization with CBFA2T3/MTG16 may be involved in regulation of the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes (PubMed:19799863). Required for the maintenance of the secretory cell lineage in the small intestine (PubMed:16227606). Can inhibit Notch signaling probably by association with RBPJ and may be involved in GFI1-mediated Paneth cell differentiation (PubMed:25398765).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate splice site and uses a downstream start codon compared to variant 1. The resulting protein (isoform 3) has a shorter N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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