Ackr2 (NM_001276719) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI464239; Ccbp2; CCR9; CCR10; Cmkbr9; D6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227374 representing NM_001276719 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCACCGTTGCTTCCCCACTGCCTCTCACCACCGTCGGTTCCGAGAACAGCAGCTCCATCTACGACT ACGACTACTTAGATGATATGACCATCTTGGTTTGCAGGAAGGACGAGGTCCTGTCCTTTGGAAGAGTCTT TCTGCCGGTCGTCTACAGCCTGATCTTCGTGCTGGGCTTGGCTGGAAACCTCCTCCTCCTGGTGGTGTTG CTCCACTCTGCACCTCGAAGACGGACGATGGAGCTTTACCTGCTGAACCTGGCCGTCTCCAACCTCTTGT TTGTAGTGACTATGCCCTTCTGGGCCATCTCTGTGGCCTGGCATTGGGTTTTTGGTAGTTTCCTGTGCAA GGTGATAAGCACTCTCTACTCTATTAACTTTTACTGTGGTATCTTCTTCATCACCTGCATGAGCCTGGAC AAATACCTGGAGATTGTCCACGCTCAGCCTCTCCACAGACCGAAGGCCCAGTTCAGGAACCTGCTTCTCA TTGTCATGGTGTGGATCACATCCCTGGCCATCTCTGTCCCAGAAATGGTCTTTGTGCAGATCCACCAGAC CTTAGATGGTGTGTGGCACTGCTATGCGGATTTTGGCGGACATGCGACCATTTGGAAGCTGTACCTGCGC TTCCAGCTGAACCTTCTGGGGTTTCTCCTCCCACTCTTGGCCATGATCTTCTTTTACTCCCGCATCGGTT GCGTTCTGGTCAGGCTGAGGCCGCCAGGCCAGGGCCGGGCTCTGAGGATGGCCGCGGCCCTGGTGATAGT TTTCTTCATGCTGTGGTTCCCATATAACCTCACCTTGTTTCTGCACTCGTTGCTGGACCTGCATGTCTTT GGGAACTGTGAGATCAGCCACCGTCTGGACTATACGTTGCAGGTGACAGAGAGCCTGGCCTTCTCCCACT GCTGCTTCACCCCGGTCCTCTACGCCTTCTGCAGTCACCGCTTCCGCCGGTACCTGAAGGCATTTCTGTC TGTGATGTTGAGATGGCACCAGGCACCTGGCACCCCTTCCTCTAACCATTCTGAGAGCAGCAGGGTTACT GCCCAGGAAGACGTGGTCAGCATGAATGACCTTGGGGAGAGGCAGTCTGAGGACTCCCTTAACAAGGGGG AGATGGGGAATACTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276719
Insert Size	1137 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276719.1, NP_001263648.1
RefSeq Size	3012 bp
RefSeq ORF	1137 bp
Locus ID	59289
UniProt ID	O08707
Gene Summary	Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines including CCL2, CCL3, CCL3L1, CCL4, CCL5, CCL7, CCL8, CCL11, CCL13, CCL17, CCL22, CCL23, CCL24, SCYA2/MCP-1, SCY3/MIP-1-alpha, SCYA5/RANTES and SCYA7/MCP-3. Upon active ligand stimulation, activates a beta-arrestin 1 (ARRB1)-dependent, G protein-independent signaling pathway that results in the phosphorylation of the actin-binding protein cofilin (CFL1) through a RAC1-PAK1-LIMK1 signaling pathway. Activation of this pathway results in up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. By scavenging chemokines in tissues, on the surfaces of lymphatic vessels, and in placenta, plays an essential role in the resolution (termination) of the inflammatory response and in the regulation of adaptive immune responses. Plays a major role in the immune silencing of macrophages during the resolution of inflammation. Acts as a regulator of inflammatory leukocyte interactions with lymphatic endothelial cells (LECs) and is required for immature/mature dendritic cells discrimination by LECs.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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