Psmd4 (NM_001282017) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Af1; angiocidin; Mcb1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227378 representing NM_001282017 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTTGGAGAGCACTATGGTTTGTGTGGACAACAGTGAGTACATGCGGAACGGAGACTTCCTTCCCA CCCGGCTGCAGGCCCAGCAGGATGCCGTCAACATTGTATGTCACTCAAAGACCCGAAGCAACCCTGAGAA TAACGTGGGCCTGATCACACTGGCCAATGACTGTGAGGTGCTGACCACACTCACCCCGGACACTGGCCGT ATCCTCTCCAAGCTCCACACTGTCCAACCCAAAGGCAAGATCACCTTCTGCACTGGCATCCGCGTGGCCC ACTTGGCTCTGAAGCACCGGCAGGGCAAGAATCACAAGATGCGCATCATCGCCTTTGTCGGTAGCCCTGT GGAGGACAACGAGAAGGATCTGGTGAAACTAGCTAAACGCCTTAAGAAAGAAAAAGTGAATGTTGACATC ATTAATTTTGGGGAAGAGGAGGTGAACACAGAGAAGCTGACAGCCTTTGTGAACACACTGAATGGCAAGG ATGGAACTGGGTCCCATCTAGTGACAGTGCCTCCTGGACCTAGCTTGGCTGATGCTCTCATCAGTTCTCC TATTCTGGCTGGTGAAGGCGGTGCCATGCTGGGTCTTGGTGCCAGTGACTTTGAGTTTGGAGTAGATCCC AGTGCTGATCCTGAATTGGCCCTGGCCCTTCGAGTCTCTATGGAAGAGCAGCGGCAGCGGCAGGAGGAAG AGGCACGGCGGGCCGCTGCGGCCTCTGCAGCTGAGGCTGGAATTGCTACACCTGGGACTGAAGGTGAAAG AGACTCGGATGACGCCCTACTGAAGATGACCATCAACCAGCAGGAGTTTGGCCGTCCTGGGCTTCCAGAC CTAAGCAGCATGACTGAGGAAGAGCAGATCGCCTACGCCATGCAGATGTCCCTGCAGGGAACAGAGTTTA GCCAAGAATCGGCTGACATGGATGCCAGCTCAGCCATGGACACATCTGATCCAGTCAAGGAGGAGGATGA CTATGACGTCATGCAGGACCCGGAGTTCCTTCAGAGCGTCCTAGAGAACCTTCCAGGTGTGGATCCCAAC AATGCAGCCATTCGAAGTGTCATGGGGGCTCTGGCCTCCCAGGCCACCAAGGATGGCAAGAATGACAAGA AAGAGGAAGAGAAGAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001282017
Insert Size	1140 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282017.1, NP_001268946.1
RefSeq Size	1344 bp
RefSeq ORF	1140 bp
Locus ID	19185
UniProt ID	O35226
Gene Summary	Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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