Lef1 (NM_001276402) Mouse Untagged Clone

CAT#: MC227420

Lef1 (untagged) - Mouse lymphoid enhancer binding factor 1 (Lef1), transcript variant 2



  "NM_001276402" in other vectors (1)

CNY 3,710.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 3000002B05; AI451430; Lef-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC227420 representing NM_001276402
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCCAACTTTCCGGAGGAGGCGGCGGGGGGGACCCGGAACTCTGCGCCACCGATGAGATGATCCCCT
TCAAGGACGAAGGCGATCCCCAGAAGGAGAAGATCTTCGCCGAGATCAGTCATCCCGAAGAGGAGGGCGA
CTTAGCCGACATCAAGTCATCTTTGGTTAACGAGTCCGAAATCATCCCAGCCAGCAACGGGCATGAGGTG
GTCAGACAAGCCCCGTCCTCTCAGGAGCCCTACCACGACAAGGCCAGAGAACACCCTGATGAAGGAAAGC
ATCCAGACGGAGGCCTGTACAACAAGGGACCCTCCTACTCCAGTTACTCTGGCTACATAATGATGCCCAA
TATGAACAGCGACCCGTACATGTCAAATGGGTCCCTTTCTCCACCCATCCCGAGGACATCAAATAAAGTG
CCCGTGGTGCAGCCCTCTCACGCGGTCCACCCGCTCACCCCCCTCATCACCTACAGCGACGAGCACTTTT
CTCCGGGATCCCACCCGTCACACATCCCGTCAGATGTCAACTCCAAGCAAGGCATGTCCAGACACCCTCC
AGCTCCTGAAATCCCCACCTTCTACCCCCTGTCTCCGGGCGGCGTTGGACAGATCACCCCACCCATTGGC
TGGTTTTCCCATCATATGATTCCTGGTCCCCCTGGCCCCCACACAACTGGCATCCCTCATCCAGCTATTG
TAACACCTCAGGTCAAACAGGAGCACCCCCACACGGACAGTGACCTAATGCACGTGAAGCCTCAACACGA
ACAGAGAAAGGAGCAGGAGCCCAAAAGACCTCATATTAAGAAGCCTCTGAATGCTTTCATGTTATATATG
AAAGAAATGAGAGCGAATGTCGTAGCTGAGTGCACGCTAAAGGAGAGTGCAGCTATCAACCAGATCCTGG
GCAGAAGATGGCACGCCCTCTCCCGGGAAGAGCAGGCCAAATACTATGAACTAGCACGGAAAGAGAGACA
GCTACACATGCAGCTTTATCCAGGCTGGTCAGCGCGAGACAATTATGGCAAGAAGAAGAAGAGGAAGAGA
GAGAAGCTACAGGAGTCGACTTCAGGTGGTAAGAGAAGCTCCTTCCCAACGTGCAAAGCCAAGGCAGCGA
CCCCAGGCCCTCTTCTGGAGATGGAAGCTTGTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001276402
Insert Size 1155 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001276402.1, NP_001263331.1
RefSeq Size 3532 bp
RefSeq ORF 1155 bp
Locus ID 16842
Gene Summary Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1 (By similarity). Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIASG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) lacks an in-frame exon in the internal coding region and lacks an exon in the 3' coding region which results in the use of a downstream stop codon, compared to variant 1. The encoded isoform (b) is shorter and has a distinct C-terminus, compared to isoform a.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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