Ift74 (NM_001290568) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1700029H06Rik; b2b796Clo; Ccdc2; Cmg1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227440 representing NM_001290568 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGTAATCACAAATCTTCAGCCCCTCGCCCTATTTCTCGGGGTGGAATAGGACTAACAGGAAGGC CTCCTTCTGGAATAAGACCTCCATCTGGCAATGTTCGAGTGGCAACTGCAATGCCACCAACAACAGCAAG ACCAGGTTCTCGTGGTGGTCCCTTAGGGACTGGTGGAGTTTTGTCATCTCAAATCAAAGTTGCTGATCGT CCTGTGACCCAACAAGGTTTGAGTGGAATGAAGACTGGCATGAAAGGTCCCCAGAGGCAAATTTTAGACA AATCTTACTATCTTGGACTTCTTAGGAGCAAAATAAGTGAACTTACAACTGAAATTAATAAGCTTCAGAA AGAAATAGAAATGTACAACCAAGAAAATTCAGTGTATTTGTCATATGAAAAGAGGGCAGAGACATTAGCT GTTGAAATTAAAGACTTTCAAGGACAACTAGCAGACTACAACATGTTGGTAGATAAACTTAATACCAATA CTGAAATGGAAGAAGTAATGAGTGATTACAACATGCTTAAAGCTCAAAATGACAGAGAAACACAAAGTAT GGATGTCATATTTACTGAAAGACAAGCGAAGGAAAAACAAATCAGAAGTGTAGAAGAAGAAGTTGAACAA GAAAAGCAAGCAGCAGATGGCATTATCAAAAATATGTCTCCTGAAAAACAAGTCAAGTACATAGAAATGA AAACCACAAATGAGAAACTGTTGCAGGAATTAGATACACTTCAGCAACAGCTAGATTCTCTGAACATGAA AAAGGAGAGCCTAGAAACTGAAATAGCACACTCCCAAGTAAAACAAGAGGCTGTATTGCTGTATGAAAAA CTTTATGAGTTAGAGTCCCATCGAGATCAAATGATTGCAGAAGACAAAAGCATGGGATCGCCAATGGAGG AAAGGGAGAGATTGCTTAAGCAGGTTAAAGAAGATAATCAGGAAATAGCCAGCATGGAGAGACAGTTAAC AGATATAAAAGAAAAAATAAACCAATTTAGTGAAGAAATTAGACAACTTGACATGGATTTAGAGGAGCAC CAAGCATGGGCCACATTGTTGCTGGGCCTGGCAGCACACACCTTGAATCCCCTCACTGAAGACAGAAGCA GGAGTGTACCGGTGAATTTGAGCACCACCCCCCTTCGGATTATATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290568
Insert Size	1167 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290568.1, NP_001277497.1
RefSeq Size	1713 bp
RefSeq ORF	1167 bp
Locus ID	67694
UniProt ID	Q8BKE9
Gene Summary	Component of the intraflagellar transport (IFT) complex B: together with IFT81, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds beta-tubulin via its basic region. Required for ciliogenesis (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks several exons in the 3' region and contains an alternate 3' terminal exon, compared to variant 1. It encodes isoform 2, which is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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