Skp2 (NM_001285980) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4930500A04Rik; FBXL1; FWD1; p45
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227443 representing NM_001285980 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGTCTCGGCCTTGGAGAAGGAGGAGGTGGACAGTGAGAACATCCCACATGGACTGCTCTCAAACC TCGGCCACCCCCAGAGCCCTCCAAGGAAACGAGTCAAGGGCAAAGGGAGTGACAAAGACTTTGTGATCAT CCGTCGGCCGAAGCTTAGTCGGGAGAACTTTCCAGGTGTCTCCTGGGACTCCCTTCCAGATGAGCTGCTC CTTGGGATCTTTTCCTGTCTGTGCCTCCCTGAGCTGCTGAGAGTCTCGGGCGTTTGCAAGAGGTGGTACC GCCTCTCGCTCGATGAGTCTCTCTGGCAGTCCCTCGACCTCGCGGGTAAAAATCTGCACCCAGACGTGAC TGTGCGCTTGCTCTCCCGCGGGGTGGTCGCCTTCCGCTGCCCTCGGTCCTTTATGGAGCAGCCGCTGGGT GAAAGCTTCAGCTCTTTCCGGGTACAGCACATGGACCTGTCGAACTCAGTGATAAATGTGTCGAACCTCC ATAAGATTCTGTCCGAGTGCTCCAAGCTGCAGAATCTAAGCCTGGAAGGCCTGCAGCTCTCAGACCCCAT TGTCAAGACTCTTGCACAGAATGAAAACTTGGTGCGACTAAACCTTTGTGGGTGCTCTGGGTTTTCTGAA TCTGCCGTGGCGACTCTGCTAAGCAGCTGCTCCAGACTGGATGAGCTAAATCTCTCCTGGTGCTTTGACT TCACTGAAAAGCACGTGCAAGCGGCTGTGGCACATTTACCAAACACCATCACCCAGCTGAACCTCAGCGG CTACCGAAAGAACCTCCAGAAAACAGATCTTTGTACCATAATTAAACGATGCCCCAACCTCATCCGCCTC GACTTAAGTGACAGTATCATGCTAAAGAATGACTGCTTTCCAGAATTTTTTCAACTCAACTACCTCCAAC ACCTCTCGCTCAGCCGGTGCTATGATATAATACCTGATACTCTACTTGAACTTGGAGAAATTCCTACACT AAAAACGCTACAAGTTTTTGGAATCGTGCCAGAGGGAACCCTTCAGCTACTGAGGGAAGCTCTTCCTCGG CTGCAGATTAACTGCGCCTATTTCACCACCATTGCAAGGCCAACTATGGACAGCAAGAAGAACCTGGAGA TTTGGGGTATCAAGTGCCGACTGACTCTGCAAAAGCCCAGTTGTCTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285980
Insert Size	1170 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285980.1, NP_001272909.1
RefSeq Size	3140 bp
RefSeq ORF	1170 bp
Locus ID	27401
Gene Summary	Substrate recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. The SCF complex provides substrate specificity and interacts with both, the E2 ubiquitin-conjugating enzyme and the substrate. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, lacks part of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (c) has a shorter N-terminus, compared to isoform a.

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