Terf1 (NM_001286628) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	P; Pin2; Trbf; Trbf1; Trf; Trf1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227458 representing NM_001286628 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGACGGTCTCCTCAGCGGCCCGGGACGCGCCGAGCCGTGAGGGCTGGACAGATTCGGATTCTC CAGAGCAGGAGGAGGTGGGAGACGACGCGGAGCTGCTCCAGTGCCAGCTTCAGCTGGGGACCCCGAGAGA GATGGAGAACGCGGAGCTTGTGGCTGAGGTGGAGGCCGTGGCTGCGGGCTGGATGCTCGACTTCCTCTGC CTGTCTCTGTGCCGAGCCTTCCGTGACGGCCGCTCCGAGGACTTTCGTCGTACTCGTGACAGCGCCGAGG CTATTATTCATGGACTACACAGACTTACAGCTTACCAATTGAAAACTGTGTATATATGTCAGTTTTTGAC AAGAGTTGCATCTGGAAAGGCCCTTGATGCACAGTTTGAAGTTGATGAGCGTATTACACCCTTGGAATCA GCCCTGATGATTTGGAACTCAATTGAAAAGGAACATGACAAACTGCATGACGAAATAAAGAATTTAATTA AAATTCAGCCTTTAGAAAGGAAGTTACTTAAGATAATCTCTCAGAAGGATGTGTTCCACTCCCTTTTCCA ACACTTCAGCTATAGCTGCATGATGGAGAAAATTCAGAGTTATGTGGGTGATGTGTTAAGTGAAAAATCA TCAACTTTTCTAATGAAGGCAGCAACAAAAGTAGTGGAAAATGAGAAAGCGAGGACACAAGCGTCTAAGG ATAGGCCAGATGCCACCAACACTGGAATGGACACTGAAGTTGGTTTGAATAAAGAGAAAAGTGTTAATGG CCAGCAGTCTACAGAAACTGAACCCTTAGTGGATACAGTATCCTCAATAAGGTCTCACAAGAACGCCTTA TCGCAGTTAAAACACAGACGTGCTCCATCAGATTTCAGTAGGAACGAAGCAAGAACAGGAACTCTTCAGT GTGAAACAACGATGGAAAGGAACCGAAGAACCAGTGGAAGGAATAGATTGTGTGTCTCAGAGAATCAGCC AGACACTGATGACAAAAGTGGACGCAGGAAAAGACAGACATGGCTTTGGGAAGAAGACAGAATTTTGAAG TGTGGTGTAAAGAAATATGGAGAGGGAAATTGGGCTAAAATACTATCCCATTATAAGTTCAACAACCGAA CAAGTGTCATGTTAAAAGATAGATGGAGAACAATGAAGAGACTGAAACTGATTAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286628
Insert Size	1179 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286628.1, NP_001273557.1
RefSeq Size	2181 bp
RefSeq ORF	1179 bp
Locus ID	21749
Gene Summary	This gene encodes a protein that binds to repeats in telomeres to form a nucleoprotein complex that protects against the degradation of chromosomal ends. The encoded protein regulates the length of telomeres and is an integral structural component of the functional telomere. This protein is thought to play a role in spindle formation in mitosis. Mutations in this gene are associated with bone marrow failure. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2013] Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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