Spn (NM_009259) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A630014B01Rik; Cd43; Galgp; Ly-48; Ly48
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227471 representing NM_009259 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTTGCACCTTCTCCTCCTCTTTGGGGCATGCTGGGTCCAGGTGGCGAGCCCAGACAGTCTGCAGA GGACGACGATGCTACCATCTACCCCACATATCACAGCTCCAAGTACCTCTGAAGCCCAGAATGCCAGCCC ATCAGTTTCTGTGGGGTCAGGGACAGTGGACTCGAAGGAGACCATCAGCCCCTGGGGGCAGACCACAATC CCAGTTTCTTTGACCCCCTTGGAAACTACTGAATTGTCTTCTTTGGAGACTTCTGCTGGTGCCAGCATGA GCACCCCTGTACCTGAGCCTACTGCCTCTCAGGAAGTTTCCAGCAAGACATCAGCACTGCTTCCAGAACC ATCAAATGTAGCCAGTGACCCTCCTGTCACTGCAGCCAATCCTGTGACAGACGGACCTGCAGCCAATCCT GTGACAGACGGAACTGCAGCATCTACATCTATCTCTAAAGGAACCAGTGCACCCCCCACCACTGTGACAA CAAGCTCTAACGAGACCAGTGGACCCTCTGTGGCTACAACAGTAAGCTCCAAGACCAGTGGTCCCCCTGT CACCACAGCTACTGGGTCTCTGGGGCCCTCGAGTGAGATGCATGGACTCCCTGCTACCACAGCAACCAGT TCTGTGGAGAGTTCCAGTGTGGCCCGTGGCACCTCAGTTTCCAGCAGAAAAACATCCACGACGTCTACCC AAGATCCCATAACCACCAGGTCACCAAGCCAAGAATCAAGTGGCATGTTACTGGTGCCCATGCTTATTGC CTTGGTGGTGGTTTTGGCCCTCGTGGCGCTACTGCTGTTGTGGCGCCAGAGGCAAAAGCGGAGGACTGGG GCCCTGACCCTGAGCGGAGGAGGAAAACGAAATGGGGTGGTGGATGCCTGGGCTGGGCCAGCCCGGGTTC CTGATGAAGAGGCCACAACCACATCAGGGGCAGGGGGCAACAAGGGCTCTGAGGTACTGGAGACAGAAGG CTCCGGGCAGAGACCCACGCTCACTACGTTCTTCAGCAGACGGAAGTCTCGCCAGGGCTCTTTAGTACTA GAAGAGCTGAAGCCTGGGTCGGGTCCCAACCTGAAGGGGGAGGAAGAGCCGCTTGTGGGCAGTGAGGATG AAGCTGTGGAAACCCCAACTTCTGACGGTCCACAAGCCAAAGATGAGGCCGCACCTCAATCTCTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_009259
Insert Size	1188 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_009259.5, NP_033285.1
RefSeq Size	3751 bp
RefSeq ORF	1188 bp
Locus ID	20737
UniProt ID	P15702
Gene Summary	Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (PubMed:17638845, PubMed:21289089, PubMed:11728336). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment (PubMed:18490738). Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells. Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (By similarity). Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin (PubMed:26700769). Acts as a T-cell counter-receptor for SIGLEC1 (PubMed:11238599).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same protein.

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