Entpd5 (NM_001286049) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI196558; AI987697; Cd39l4; ER-UDPase; mNTPase; NTPDase-5; NTPDase5; Pcph
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227637 representing NM_001286049 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACTTCCTGGGGGGCTGTCTTCATGCTGATCATAGCCTGCGTTGGCAGCACTGTCTTCTACAGAG AACAGCAGACCTGGTTTGAAGGTGTCTTCTTGTCTTCCATGTGCCCCATTAATGTCAGTGCCGGCACCTT TTATGGAATTATGTTTGATGCGGGCAGCACTGGAACTCGGATTCATGTTTACACTTTTGTGCAGAAAACA GCAGGACAGCTCCCCTTTCTGGAAGGTGAAATTTTTGATTCTGTGAAGCCGGGACTTTCTGCTTTTGTGG ATCAGCCCAAACAGGGTGCTGAGACTGTCCAGGAGCTCTTGGAGGTGGCCAAAGACTCGATCCCCAGAAG CCACTGGGAAAGGACCCCGGTGGTTCTGAAAGCAACGGCCGGACTCCGTTTGCTGCCTGAGCAGAAAGCC CAGGCTCTGCTCTTGGAGGTAGAGGAGATCTTCAAGAATTCACCTTTCCTGGTCCCAGATGGCAGCGTTA GCATCATGGATGGGTCCTATGAAGGCATACTAGCCTGGGTTACCGTGAACTTTCTAACAGGTCAGCTGCA TGGTCGTGGCCAGGAGACTGTGGGGACCCTTGACCTGGGGGGTGCCTCCACCCAAATCACGTTTCTACCC CAGTTTGAGAAAACCCTGGAACAAACACCTAGGGGCTACCTCACTTCCTTTGAGATGTTTAACAGCACTT TTAAGCTCTATACACATAGTTACTTGGGATTTGGACTGAAAGCTGCAAGACTGGCAACTCTGGGAGCCCT GGAAGCAAAAGGGACTGATGGACATACGTTTCGAAGTGCCTGTTTACCAAGATGGTTGGAAGCAGAGTGG ATCTTTGGGGGTGTGAAATACCAGTATGGTGGTAACCAAGAAGGGGAGATGGGCTTTGAACCCTGCTATG CGGAAGTGCTGAGGGTAGTACAGGGGAAACTTCACCAGCCAGAAGAAGTCCGAGGAAGCGCCTTCTACGC TTTCTCTTACTACTACGATCGAGCCGCTGACACACACTTGATCGATTATGAAAAGGGCGGGGTTTTAAAA GTTGAAGATTTTGAAAGAAAAGCCAGAGAAGTGTGTGACAACTTGGGGAGCTTCTCCTCGGGCAGTCCTT TCCTCTGCATGGACCTCACTTACATCACAGCCCTGTTGAAAGATGGTTTTGGCTTTGCCGACGGCACCCT CTTACAGCTCACAAAGAAAGTGAACAACATAGAGACTGGTTGGGCCTTGGGGGCCACCTTTCACCTGCTC CAGTCTCTGGGCATCACCAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286049
Insert Size	1284 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286049.1, NP_001272978.1
RefSeq Size	4962 bp
RefSeq ORF	1284 bp
Locus ID	12499
UniProt ID	Q9WUZ9
Gene Summary	Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. The nucleotide hydrolyzing preference is GDP > IDP > UDP, but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 2. It encodes the predominant protein (isoform b). Variants 2, 3 and 4 encode the same isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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