Ddx39b (NM_001252457) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	0610030D10Rik; AI428441; Bat-1; Bat1; Bat1a; D6S81Eh; D17H6S81E; D17H6S81E-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227645 representing NM_001252457 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAGAACGATGTGGACAATGAGCTCTTGGACTACGAAGACGACGAGGTGGAGACAGCCGCTGGGG CAGATGGGACCGAGGCTCCCGCCAAGAAAGACGTCAAGGGCTCCTACGTCTCCATCCACAGCTCCGGCTT CCGAGATTTTCTACTCAAGCCAGAGCTGCTCCGGGCCATCGTTGACTGTGGCTTTGAGCATCCATCAGAG GTCCAGCATGAGTGCATCCCGCAGGCCATTCTGGGGATGGATGTCCTGTGCCAGGCCAAGTCAGGCATGG GAAAAACAGCAGTGTTTGTCCTGGCCACACTGCAGCAGCTGGAGCCCGTTACTGGGCAGGTGTCTGTGCT GGTGATGTGTCACACTAGGGAGCTGGCTTTTCAGATCAGCAAGGAATATGAGCGCTTCTCTAAGTACATG CCGAATGTCAAGGTGGCAGTGTTTTTTGGCGGTCTGTCTATCAAGAAGGACGAAGAGGTGCTGAAGAAGA ACTGCCCACACATCGTCGTGGGGACTCCTGGCCGAATTCTAGCCCTGGCTCGAAATAAGAGCCTGAACCT CAAACACATTAAACACTTTATTTTGGACGAGTGTGACAAGATGCTTGAACAGCTCGACATGCGTCGGGAT GTCCAGGAAATTTTTCGCATGACCCCCCATGAGAAGCAGGTCATGATGTTCAGTGCTACCTTGAGCAAAG AGATCCGCCCAGTCTGCCGCAAGTTCATGCAAGATCCTATGGAGATCTTCGTGGATGACGAGACCAAGTT GACGCTGCACGGGTTGCAGCAGTACTACGTGAAACTGAAGGACAACGAGAAGAACCGGAAGCTCTTTGAT CTTCTCGATGTCCTCGAGTTCAACCAGGTGGTGATCTTTGTGAAGTCCGTGCAGCGCTGCATCGCCCTGG CCCAGCTTCTAGTGGAACAGAACTTCCCAGCCATTGCTATCCATCGTGGAATGCCCCAGGAGGAGAGGCT CTCTCGGTATCAGCAGTTCAAGGATTTTCAGCGGAGGATTCTTGTGGCTACCAACCTGTTTGGCCGAGGC ATGGATATTGAGCGTGTGAACATTGCTTTCAACTATGACATGCCAGAGGACTCGGACACCTACCTGCACA GGGTGGCCAGAGCGGGCCGGTTTGGCACCAAGGGCTTGGCCATCACATTTGTGTCAGATGAGAATGATGC CAAGATCCTGAATGACGTTCAGGACCGTTTCGAGGTCAACATCAGCGAGCTGCCCGATGAGATTGACATT TCCTCCTACATTGAGCAGACACGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252457
Insert Size	1287 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252457.1, NP_001239386.1
RefSeq Size	1651 bp
RefSeq ORF	1287 bp
Locus ID	53817
UniProt ID	Q9Z1N5
Gene Summary	Involved in nuclear export of spliced and unspliced mRNA. Assembling component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. May undergo several rounds of ATP hydrolysis during assembly of TREX to drive subsequent loading of components such as ALYREF/THOC and CHTOP onto mRNA. Also associates with pre-mRNA independent of ALYREF/THOC4 and the THO complex. Involved in the nuclear export of intronless mRNA; the ATP-bound form is proposed to recruit export adapter ALYREF/THOC4 to intronless mRNA; its ATPase activity is cooperatively stimulated by RNA and ALYREF/THOC4 and ATP hydrolysis is thought to trigger the dissociation from RNA to allow the association of ALYREF/THOC4 and the NXF1-NXT1 heterodimer. Involved in transcription elongation and genome stability (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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