Tfap2a (NM_001301674) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A; AP; AP-2; Ap-2 (a); Ap2; AP2alpha; Ap2tf; Tcfa; Tcfap2a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227696 representing NM_001301674 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTTGGAAACTGACGGATAATATCAAGTACGAAGACTGCGAGGACCGTCACGACGGCACCAGCAACG GGACGGCACGGTTGCCCCAGCTGGGCACTGTAGGTCAATCTCCCTACACCAGCGCCCCGCCGCTGTCCCA CACCCCTAATGCCGACTTCCAGCCTCCCTACTTCCCCCCGCCCTACCAGCCTATCTACCCCCAGTCGCAA GATCCTTACTCCCACGTCAACGACCCCTACAGCCTGAATCCTCTGCACGCCCAGCCGCAGCCGCAGCACC CGGGCTGGCCCGGTCAGAGGCAGAGCCAGGAATCTGGGCTCTTACACACACACCGGGGCTTACCCCACCA ACTGTCGGGCCTGGACCCTCGCAGGGACTATCGGCGGCACGAGGACCTCTTGCACGGCCCGCACGGGCTC GGCTCTGGGCTCGGGGACCTCCCGATCCACTCCTTACCTCACGCCATCGAGGACGTCCCGCACGTAGAAG ACCCGGGTATTAACATCCCAGATCAAACTGTAATTAAGAAAGGCCCCGTGTCCCTGTCCAAGTCCAACAG CAATGCTGTCTCCGCCATCCCTATTAACAAGGACAACCTCTTCGGTGGCGTGGTAAACCCCAACGAAGTC TTCTGTTCAGTTCCGGGTCGCCTGTCGCTCCTCAGCTCCACCTCGAAGTACAAGGTCACGGTGGCGGAAG TACAGCGGCGCCTCTCACCGCCCGAGTGTCTTAACGCGTCGCTCCTGGGCGGAGTACTGCGGAGAGCGAA GTCTAAGAATGGAGGGAGATCTTTAAGAGAAAAACTGGACAAGATAGGATTGAATCTGCCAGCAGGGAGA CGTAAAGCTGCCAACGTTACCCTCCTCACGTCACTAGTGGAAGGAGAAGCCGTCCACCTAGCCAGGGACT TTGGGTACGTGTGCGAAACTGAATTTCCTGCCAAAGCAGTAGCAGAATTTCTCAACCGACAACATTCCGA TCCCAATGAGCAAGTGGCAAGAAAAAACATGCTCCTGGCCACAAAACAGATCTGCAAAGAGTTCACTGAC CTGCTGGCTCAGGACCGATCTCCCCTGGGGAACTCGCGGCCCAATCCTATCCTGGAGCCTGGCATCCAGA GTTGCTTGACCCACTTCAACCTCATCTCCCATGGCTTCGGCAGCCCGGCGGTGTGTGCAGCGGTTACGGC CCTGCAGAACTATCTCACCGAGGCCCTCAAGGCCATGGACAAAATGTACCTCAGCAACAACCCCAACAGC CACACGGACAACAGCGCCAAAAGCAGTGACAAAGAAGAGAAACACAGAAAGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001301674
Insert Size	1314 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301674.1, NP_001288603.1
RefSeq Size	3479 bp
RefSeq ORF	1320 bp
Locus ID	21418
UniProt ID	P34056
Gene Summary	This gene is a member of the activator protein 2 (AP-2) transcription factor family. The protein encoded by this gene can act as both an activator and repressor of gene transcription, and plays an important role in early embryogenesis, specifically in cranial development. This protein forms both homodimers and heterodimers, and binds to a GC-rich consensus sequence found in some promoters and enhancers. Disruption of this gene causes perinatal death, with neural tube, craniofacial, and limb mesenchyme defects. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms. [provided by RefSeq, Sep 2014] Transcript Variant: This variant (1b) differs in the 5' UTR compared to variant 1a. Both variants 1a and 1b encode the same isoform (1).

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