Tead2 (NM_001285498) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Etdf; ETF; TEAD-2; TEF-4; TEF4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227737 representing NM_001285498 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGATCCCCGGACTGGGGCCCCCCTGGATGATGGCGGGGGCTGGACAGGTAGCGAGGAAGGCAGCG AAGAGGGCACCGGAGGCAGCGAGGGCGTTGGAGGTGATGGCAGCCCTGATGCAGAAGGTGTGTGGAGCCC CGACATTGAGCAGAGTTTTCAGGAGGCCCTGGCCATCTACCCTCCCTGTGGCCGTCGCAAGATCATCCTG TCAGATGAGGGCAAGATGTATGGCCGGAACGAACTCATTGCCCGTTACATCAAGCTGAGGACAGGGAAGA CGAGAACGCGAAAGCAGGTCTCCAGCCATATTCAGGTTTTGGCTCGAAGGAAATCGAGAGAAATTCAGTC CAAGCTGAAGGACCAAGTCTCCAAGGACAAGGCCTTCCAGACGATGGCCACCATGTCTTCGGCACAGCTC ATCTCCGCCCCTTCCCTCCAGGCCAAGCTGGGCCCTTCTGGCCCTCAGGCCACTGAGCTTTTCCAGTTCT GGTCAGGGAGCTCTGGGCCACCATGGAATGTTCCAGACGTGAAGCCCTTCTCACAGGCACCGTTCTCCGT GTCACTGACGCCCCCAGCCTCTGACCTACCAGGGTACGAGCCGCCCCCAGCCCTCTCACCCCTGCCCCCA CCCGCTCCGTCTCCCCCAGCCTGGCAGGCTCGGGCCCTGGGCACTGCCCGCCTGCAGCTGATAGAGTTCT CAGCGTTTGTGGAACCGCCAGACGCAGTTGACTCGTTCCAGAGGCATCTGTTTGTCCACATCAGTCAGCA GTGTCCCAGCCCTGGAGCACCACCCCTAGAGAGTGTGGACGTGCGGCAGATCTACGACAAATTCCCTGAG AAGAAGGGCGGCCTCCGCGAGCTGTATGACCGAGGGCCACCACATGCCTTCTTCCTCGTCAAGTTCTGGG CGGACCTGAACTGGGGCCCCAGTGCCGAGGAGGCAGGGAGCAGCGGAGGTGGCGGTGGCTTCTATGGAGT GAGCAGCCAGTATGAGAGCCGGGAGCTCATGACACTCACCTGCTCCTCCAAGGTCTGCTCCTTTGGCAAG CAAGTGGTAGAGAAGGTGGAGACGGAACGGGCCCAGCTGGAGGACGGGCGCTTTGTGTACCGTCTGCTGC GCTCTCCCATGTGTGAGTACCTGGTTAATTTCCTGCACAAGCTCCGTCAGCTGCCTGAACGCTACATGAT GAACAGTGTCCTGGAGAACTTCACCATCCTCCAGGTTGTGACAAACAGGGACACTCAGGAACTGCTGCTG TGTACTGCCTACGTCTTTGAAGTCTCCACCAGTGAACGAGGAGCCCAGTACCACATCTACCGCCTGGTCA GGGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_001285498
Insert Size	1338 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285498.1, NP_001272427.1
RefSeq Size	2157 bp
RefSeq ORF	1338 bp
Locus ID	21677
UniProt ID	P48301
Gene Summary	Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction (By similarity). Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1 and 2 both encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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