Nprl3 (NM_001284360) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Aag; CGTHBA; HS-26; HS-40; m(alpha)RE; Mare; Phg; Prox1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227760 representing NM_001284360 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTCTTTTTAACGTGGTATTTGCACTGAGGGCCAATGCTGATCCATCAGTGATAAACTGTCTACACA ACTTGTCCCGCCGAATTGCTACTGTCCTGCAGCATGAGGAGCGCCGCTGCCAGTACCTCACTCGAGAGGC CAAGCTGATCCTAGCACTTCAGGATGAGGTGTCTGCTATGGCTGATGCAAATGAAGGTCCTCAGTCCCCA TTCCAGCACATCCTACCCAAGTGCAAGCTGGCTCGGGACCTCAAAGAAGCTTATGACAGCCTGTGCACAT CTGGTGTGGTACGGCTTCACATTAACAGCTGGCTGGAAGTGAGTTTCTGTCTGCCCCACAAGATCCACTA TGCAGCTTCAAGTCTGATTCCTCCTGAGGCTATTGAACGGAGCCTGAAAGCCATCCGCCCGTACCATGCC TTGCTACTTCTCAGTGACGAGAAGTCTCTGCTGAGTGAGCTTCCCATTGACTGCTCCCCGGCTCTGGTGC GGGTGATCAAGACCACGTCTGCTGTGAAGAACCTGCAGCAGCTAGCCCAGGATGCCGATCTGGCCTTGCT GCAGGTCTTCCAGTTGGCAGCTCACCTGGTATACTGGGGCAAGGCTGTCATCATCTACCCACTGTGTGAG AACAACGTCTATGTCATGTCTCCCAATGCCAGTGTGTGTCTGTACTCCCCGCTAGCTGAACAGTTCTCCC GGCAGTTCCCGTCCCATGACCTGCCATCTGTCCTGGCCAAGTTTTCCTTGCCTGTTTCTTTATCGGAGTT CAGGAGCCCTCTGGCTCCCCCTGCACAGGAGACCCAGCTCATCCAGATGGTGGTGTGGATGCTGCAGCGC CGGCTCCTCATCCAGCTGCATACCTATGTTTGCCTAATGGCCTCACCCAGCGAAGAGGAGCCCCGACTGC GAGAGGATGATGTCCCCTTCACAGCCAGAGTTGGTGGCCGCAGCCTCAGCACACCCAATGCTCTAAGCTT TGGCTCCCCAACCAGCAGTGACGACATGACCCTTACCAGCCCCAGTATGGACAACTCAAGTGCTGAGCTG CTCCCCAGTGGGGACTCACCACTGAACAAGAGGATGACAGAGAACCTGCTGGCTAGCCTCTCAGAGCATG AGCGGGCTGCTATCCTCAATGTGCCTGCAGCCCAAAACCCTGAGGACCTCCGCATGTTTGCCAGGCTCCT TCACTACTTCCGTGGCCGCCACCATCTGGAGGAGATCATGTACAATGAGAACACTCGGCGCTCCCAGCTA CTCATGCTCTTTGACAAATTCCGCAGTGTGCTGGTGGTGACCACCCATGAGGACCCTGTTATTGCCGTCT TCCAGGCACTGCTCACATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001284360
Insert Size	1350 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001284360.1, NP_001271289.1
RefSeq Size	2735 bp
RefSeq ORF	1350 bp
Locus ID	17168
UniProt ID	Q8VIJ8
Gene Summary	As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks two alternate 5' coding exons, which causes translation initiation at a downstream start codon, compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1.

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