Nprl3 (NM_001284360) Mouse Untagged Clone

CAT#: MC227760

Nprl3 (untagged) - Mouse nitrogen permease regulator-like 3 (Nprl3), transcript variant 3



  "NM_001284360" in other vectors (1)

CNY 4,370.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Aag; CGTHBA; HS-26; HS-40; m(alpha)RE; Mare; Phg; Prox1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC227760 representing NM_001284360
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTCTTTTTAACGTGGTATTTGCACTGAGGGCCAATGCTGATCCATCAGTGATAAACTGTCTACACA
ACTTGTCCCGCCGAATTGCTACTGTCCTGCAGCATGAGGAGCGCCGCTGCCAGTACCTCACTCGAGAGGC
CAAGCTGATCCTAGCACTTCAGGATGAGGTGTCTGCTATGGCTGATGCAAATGAAGGTCCTCAGTCCCCA
TTCCAGCACATCCTACCCAAGTGCAAGCTGGCTCGGGACCTCAAAGAAGCTTATGACAGCCTGTGCACAT
CTGGTGTGGTACGGCTTCACATTAACAGCTGGCTGGAAGTGAGTTTCTGTCTGCCCCACAAGATCCACTA
TGCAGCTTCAAGTCTGATTCCTCCTGAGGCTATTGAACGGAGCCTGAAAGCCATCCGCCCGTACCATGCC
TTGCTACTTCTCAGTGACGAGAAGTCTCTGCTGAGTGAGCTTCCCATTGACTGCTCCCCGGCTCTGGTGC
GGGTGATCAAGACCACGTCTGCTGTGAAGAACCTGCAGCAGCTAGCCCAGGATGCCGATCTGGCCTTGCT
GCAGGTCTTCCAGTTGGCAGCTCACCTGGTATACTGGGGCAAGGCTGTCATCATCTACCCACTGTGTGAG
AACAACGTCTATGTCATGTCTCCCAATGCCAGTGTGTGTCTGTACTCCCCGCTAGCTGAACAGTTCTCCC
GGCAGTTCCCGTCCCATGACCTGCCATCTGTCCTGGCCAAGTTTTCCTTGCCTGTTTCTTTATCGGAGTT
CAGGAGCCCTCTGGCTCCCCCTGCACAGGAGACCCAGCTCATCCAGATGGTGGTGTGGATGCTGCAGCGC
CGGCTCCTCATCCAGCTGCATACCTATGTTTGCCTAATGGCCTCACCCAGCGAAGAGGAGCCCCGACTGC
GAGAGGATGATGTCCCCTTCACAGCCAGAGTTGGTGGCCGCAGCCTCAGCACACCCAATGCTCTAAGCTT
TGGCTCCCCAACCAGCAGTGACGACATGACCCTTACCAGCCCCAGTATGGACAACTCAAGTGCTGAGCTG
CTCCCCAGTGGGGACTCACCACTGAACAAGAGGATGACAGAGAACCTGCTGGCTAGCCTCTCAGAGCATG
AGCGGGCTGCTATCCTCAATGTGCCTGCAGCCCAAAACCCTGAGGACCTCCGCATGTTTGCCAGGCTCCT
TCACTACTTCCGTGGCCGCCACCATCTGGAGGAGATCATGTACAATGAGAACACTCGGCGCTCCCAGCTA
CTCATGCTCTTTGACAAATTCCGCAGTGTGCTGGTGGTGACCACCCATGAGGACCCTGTTATTGCCGTCT
TCCAGGCACTGCTCACATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001284360
Insert Size 1350 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001284360.1, NP_001271289.1
RefSeq Size 2735 bp
RefSeq ORF 1350 bp
Locus ID 17168
UniProt ID Q8VIJ8
Gene Summary As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) lacks two alternate 5' coding exons, which causes translation initiation at a downstream start codon, compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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