Plin5 (NM_025874) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2310076L09Rik; AI415325; AW109675; Lsdp5; MLDP; PAT-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227824 representing NM_025874 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCAGAGAGGTGAAGACACCACCCTAGCGCCACACAGCAGAATGTCCGGTGATCAGACAGCTCAGG ACCCTGGATCCAGCCTGGGAGAACTGGACCAGCAGAATGTGGTGAATCGAGTGGTGGCTTTGCCCCTGGT CAAGGCCACGTGCACTGCCGTGTCCAGTGCTTACAACTCGGCCAAGGACAGGCACCCGCTGCTGGGCTCC GCCTGCCGCCTTGCTGAGCACTGTGTGTGTAGTGTGACTACCTGTGCCCTGGACCACGCACAGCCACTGC TGGAGCACCTGCAGCCCCAGTTGGCCACAGTGAATGATCTTGCCTGCAGGGGACTAGACAAATTGGAAGA GAAGCTGCCCTTCCTGCAGCAGCCATCAGACATGGTGGTGACATCAGCCAAGGATACAGTGGCCAAAAGT GTCACAGGCATGGTGGACCTGGCCCAAAGGGGCCGGCGTTGGAGTGGGGAGCTGAGGCGCTCCATGAGTC AAGCCATGGACATGGTGCTGGGCAAGTCGGAGAAGCTGGTGGACCGCTTCCTGCCCATGACTGAGGCTGA GCTAGCAGTCCTGGCAGCTGAGGCCGAGGGCCCAGAAGTGGGCACAGTGGAGGAGCAGAGGCAGCAACAG GGCTACTTTGTGCGTCTGGGGTCCCTATCGGCACGCCTCCGCCATCTCGCCTATGAACACTCTTTGGGGA AACTGAGGCAGAGCAAACACCGTACCCAGGAGATGCTGGCCCAGCTGCAGGAAACGCTGGAGCTGATCCA GCATATGCAGAGAGGGGCAAGCCCTAGCCCTACTTTCCATCCCCCAAAGACTCAGGAGCTGTGGGGGAGC TGGAGCCCGTGTCTAGAGAATGGCCGCAGCCACAGTGAGGTGGAGCTGGAGACACTGGCTCTGTCTCGAA GTTTGACCCTGGAGCTGCAGAATGCAGTGGATGCCCTGGCAGGCTGTGTTCGGGGCCTGCCACCTAGTGC CCAGGCCAAGGTGGCTGAGGTGCAGCGCAGCGTGGATGCTCTACAGGCCACCTTTGCTGATGCACACTGC CTTGGTGATGTGGCACCCACTGCTCTGGCTGAGGGCCGGGGCAGTGTGGCCCGGGCACATGCCTGTGTGG ATGAGTTCCTGGATTTGGTCCTGCGGGCCATGCCACTGCCCTGGCTTGTGGGGCCCTTTGCACCCATCCT GGTGGAACAGTCGGAGCCCCTGATCAACCTGGCCACCTGTGTGGACGAGGTGGTGGGTGACCCTGATCCT CGCTGGGCACACATGGACTGGCCAGCCCAGAAGAGGGCCTGGGAGGCTGAGTCTGCAGATCCTGGGGGGC AAGAGGCTGAGCCCCCAAGGGGGCAAGGCAAGCACACAATGATGCCAGAGCTGGACTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_025874
Insert Size	1392 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_025874.3, NP_080150.2
RefSeq Size	1940 bp
RefSeq ORF	1392 bp
Locus ID	66968
UniProt ID	Q8BVZ1
Gene Summary	Lipid droplet-associated protein that maintains the balance between lipogenesis and lipolysis and also regulates fatty acid oxidation in oxidative tissues. Recruits mitochondria to the surface of lipid droplets and is involved in lipid droplet homeostasis by regulating both the storage of fatty acids in the form of triglycerides and the release of fatty acids for mitochondrial fatty acid oxidation. In lipid droplet triacylglycerol hydrolysis, plays a role as a scaffolding protein for three major key lipolytic players: ABHD5, PNPLA2 and LIPE. Reduces the triacylglycerol hydrolase activity of PNPLA2 by recruiting and sequestering PNPLA2 to lipid droplets. Phosphorylation by PKA enables lipolysis probably by promoting release of ABHD5 from the perilipin scaffold and by facilitating interaction of ABHD5 with PNPLA2. Also increases lipolysis through interaction with LIPE and upon PKA-mediated phosphorylation of LIPE.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the predominant transcript. Variants 1 and 2 encode the same protein.

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