Smad2 (NM_001252481) Mouse Untagged Clone

CAT#: MC227845

Smad2 (untagged) - Mouse SMAD family member 2 (Smad2), transcript variant 2



  "NM_001252481" in other vectors (1)

CNY 4,470.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 7120426M23Rik; Madh2; Madr2; mMad2; Smad-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC227845 representing NM_001252481
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGTCCATCTTGCCATTCACTCCGCCAGTGGTGAAGAGACTTCTGGGATGGAAAAAATCAGCCGGTG
GGTCTGGAGGAGCAGGTGGTGGAGAGCAGAATGGACAGGAAGAAAAGTGGTGTGAAAAAGCAGTGAAAAG
TCTGGTGAAAAAGCTAAAGAAAACAGGACGGTTAGATGAGCTTGAGAAAGCCATCACCACTCAGAATTGC
AATACTAAATGTGTCACCATACCAAGCACTTGCTCTGAAATTTGGGGACTGAGTACAGCAAATACGGTAG
ATCAGTGGGACACAACAGGCCTTTACAGCTTCTCTGAACAAACCAGGTCTCTTGATGGCCGTCTTCAGGT
TTCACACCGGAAAGGGTTGCCACATGTTATATATTGCCGGCTCTGGCGCTGGCCGGACCTTCACAGTCAT
CATGAGCTCAAGGCAATCGAAAACTGCGAATATGCTTTTAATCTGAAAAAAGATGAAGTGTGTGTAAATC
CGTACCACTACCAGAGAGTTGAGACCCCAGTCTTGCCTCCAGTCTTAGTGCCTCGGCACACGGAGATTCT
AACAGAACTGCCGCCCCTGGATGACTACACCCACTCCATTCCAGAAAACACAAATTTCCCAGCAGGAATT
GAGCCACAGAGTAATTACATCCCAGAAACACCACCACCTGGATATATCAGTGAAGATGGAGAAACAAGTG
ACCAACAGTTGAACCAAAGTATGGACACAGGCTCTCCGGCTGAACTGTCTCCTACTACTCTCTCTCCTGT
TAATCACAGCTTGGATTTGCAGCCAGTTACTTACTCGGAACCTGCATTCTGGTGTTCAATCGCATACTAT
GAACTAAACCAGAGGGTTGGAGAGACCTTCCATGCGTCACAGCCCTCGCTCACTGTAGACGGCTTCACAG
ACCCATCAAACTCGGAGAGGTTCTGCTTAGGCTTGCTCTCCAACGTTAACCGAAATGCCACTGTAGAAAT
GACAAGAAGACATATAGGAAGGGGAGTGCGCTTGTATTACATAGGTGGGGAAGTGTTTGCTGAGTGCCTA
AGTGATAGTGCAATCTTTGTGCAGAGCCCCAACTGTAACCAGAGATACGGCTGGCACCCTGCAACAGTGT
GTAAGATCCCACCAGGCTGTAACCTGAAGATCTTCAACAACCAAGAATTTGCTGCTCTTCTGGCTCAGTC
TGTCAACCAGGGTTTTGAAGCCGTTTATCAGCTAACCCGAATGTGCACCATAAGAATGAGTTTTGTGAAG
GGCTGGGGAGCAGAATATCGGAGGCAGACAGTAACAAGTACTCCTTGCTGGATTGAACTTCATCTGAATG
GCCCTCTGCAGTGGCTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGTGCGATGCTCAAGCATGTC
GTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001252481
Insert Size 1404 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001252481.1, NP_001239410.1
RefSeq Size 8863 bp
RefSeq ORF 1404 bp
Locus ID 17126
UniProt ID Q62432
Gene Summary Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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