Tfe3 (NM_001271490) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	bHLHe33; F830016E06Rik; Tcfe3; Tfe-3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227849 representing NM_001271490 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCATCTTCTTCATCACGGGTCTTGCTGCGTCAGCAGCTTATGAGGGCCCAGGCTCAGGAACAGGAGA GGCGTGAGCGGCGGGAACAGGCAGCGGCCGCTCCCTTCCCCAGCCCTGCGCCCGCCTCACCAGCCATCTC TGTGATTGGCGTGTCTGCTGGTGGCCACACACTGAGTCGTCCACCTCCTGCTCAGGTGCCCAGGGAGGTA CTCAAGGTTCAGACCCACCTCGAGAACCCTACACGCTACCACCTGCAGCAAGCTCGCCGGCAGCAGGTCA AACAGTACTTGTCTACCACACTTGGGCCCAAGCTGGCTTCCCAGGCTCTCACCCCACCACCAGGGCCTTC CAGTGCCCAGCCACTTCCTGCCCCTGAAACTGCTCATGCCACCGGTCCTACAGGCAGTGCTCCTAACAGC CCCATGGCGCTGCTCACCATTGGGTCCAGCTCAGAAAAGGAGATTGATGATGTCATTGATGAGATCATCA GCCTGGAGTCCAGTTACAACGATGAGATGCTCAGCTATCTTCCAGGAGGCACTGCAGGGCTGCAGCTCCC CAGCACGCTGCCGGTGTCTGGAAATCTACTTGATGTGTACAGCAGTCAGGGAGTGGCTACCCCAGCTATC ACCGTCAGCAATTCCTGTCCAGCTGAGCTGCCTAACATCAAACGCGAGATCTCCGAAACCGAGGCAAAGG CCCTTTTGAAGGAGCGACAGAAGAAAGACAATCACAACCTAATTGAACGACGCAGGCGATTCAACATTAA CGATAGGATCAAAGAGCTGGGCACCCTCATCCCCAAGTCCAATGATCCGGAGATGCGCTGGAACAAGGGC ACCATCCTGAAGGCATCTGTGGATTACATCCGCAAATTACAGAAGGAACAGCAACGCTCCAAAGACCTGG AGAGCCGGCAGCGGTCCCTGGAACAAGCCAACCGAAGCCTGCAGCTCCGGATTCAGGAGCTAGAACTGCA GGCCCAGATCCATGGTCTGCCAGTCCCTCCCAACCCAGGACTGCTCTCCCTAACCACGAGTTCGGTCTCT GACAGCCTCAAGCCAGAACAGCTGGACATTGAGGAGGAGGGCAGGCCTAGCACAACGTTCCATGTATCGG GAGGACCTGCCCAGAACGCTCCTCCACAACAGCCTCCAGCACCACCTTCGGATGCCCTTCTGGACCTACA CTTTCCCAGCGACCACTTGGGAGACCTGGGGGACCCCTTCCACCTAGGGCTAGAGGACATTCTGATGGAG GAGGAGGGGATGGTGGGAGGACTGTCAGGGGGTGCCCTGTCCCCACTGCGGGCTGCTTCTGACCCCCTGC TTTCTTCAGTATCCCCGGCTGTTTCCAAGGCCAGCAGCCGCCGTAGCAGCTTCAGCATGGAAGAGGAGTC CTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271490
Insert Size	1404 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271490.1, NP_001258419.1
RefSeq Size	3446 bp
RefSeq ORF	1404 bp
Locus ID	209446
UniProt ID	Q64092
Gene Summary	Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3') (PubMed:16936731). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF (PubMed:16936731). In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity (PubMed:16936731). Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer (By similarity). It also binds very well to a USF/MLTF site (By similarity). May regulate lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1 (PubMed:29146937).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) uses an alternate splice site in the 5' exon, compared to variant 1, and initiates translation at a downstream in-frame start codon. The encoded isoform (c) has a shorter N-terminus, compared to isoform a. Variants 3, 4, and 5 encode the same isoform (c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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