Slc25a25 (NM_001290558) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110030N17Rik; MCSC; mKIAA1896
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227856 representing NM_001290558 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCTGCCTGTGCCTGTATGTGCCCATCGCCGGGGCGGCTCAGACTGAGTTCCAGTACTTTGAGTCCA AGGGGCTTCCTGCCGAGCTGAAATCCATCTTCAAACTCAGTGTCTTTATCCCCTCTCAAGAGTTCTCCAC ATACCGCCAATGGAAGCAGAAAATTGTGCAAGCAGGTGACAAGGACCTTGATGGGCAACTGGACTTTGAA GAGTTTGTACATTACCTCCAAGATCATGAGAAAAAACTGAGGCTGGTGTTCAAGAGTCTGGACAAAAAGA ATGATGGTCGAATCGATGCTCAGGAGATCATGCAGTCCCTGCGGGACCTGGGTGTCAAGATCTCGGAACA GCAGGCGGAGAAGATTCTTAAGAGCATGGATAAGAATGGCACGATGACCATCGACTGGAACGAGTGGAGG GACTACCACCTCCTGCACCCTGTGGAGAACATCCCGGAGATCATCCTGTACTGGAAGCACTCGACGATCT TCGATGTCGGTGAGAATCTGACAGTCCCAGATGAGTTCACAGTGGAGGAGAGGCAGACGGGGATGTGGTG GAGGCACCTGGTGGCAGGAGGTGGGGCAGGGGCAGTTTCCAGAACCTGCACTGCCCCCCTGGACAGACTG AAGGTGCTCATGCAGGTCCATGCCTCCCGCAGCAACAACATGTGCATCGTAGGTGGATTCACACAGATGA TTCGAGAAGGGGGAGCCAAGTCACTCTGGCGGGGCAACGGCATCAATGTCCTCAAAATTGCCCCTGAGTC GGCCATCAAATTCATGGCATATGAGCAGATGAAACGGCTTGTCGGTAGTGATCAGGAGACGCTGAGGATC CACGAAAGGCTTGTGGCAGGCTCCTTGGCCGGAGCCATTGCCCAGAGTAGCATCTACCCAATGGAGGTTC TGAAGACCCGAATGGCCCTGCGGAAAACAGGACAGTACTCCGGCATGCTGGACTGTGCCAGGAGGATCTT GGCTAAAGAGGGTGTAGCTGCCTTCTACAAAGGCTACATCCCCAACATGCTGGGGATCATCCCCTATGCT GGCATCGACCTAGCTGTCTATGAGACATTGAAAAATACCTGGCTCCAGCGCTACGCAGTAAACAGTGCAG ACCCCGGTGTGTTCGTGCTCCTGGCCTGTGGTACTATCTCCAGTACTTGTGGCCAGCTGGCCAGCTACCC ACTAGCCCTGGTCAGGACCCGGATGCAGGCACAAGCCTCCATTGAGGGCGCACCTGAGGTAACCATGAGC AGCCTCTTCAAACAGATTCTGCGGACTGAGGGGGCCTTTGGGCTCTACCGGGGGCTGGCCCCCAACTTCA TGAAGGTGATCCCGGCTGTGAGCATCAGCTACGTGGTCTACGAAAACCTGAAGATCACCCTGGGCGTGCA GTCTCGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290558
Insert Size	1410 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290558.1, NP_001277487.1
RefSeq Size	3195 bp
RefSeq ORF	1410 bp
Locus ID	227731
UniProt ID	A2ASZ8
Gene Summary	Calcium-dependent mitochondrial solute carrier. Mitochondrial solute carriers shuttle metabolites, nucleotides, and cofactors through the mitochondrial inner membrane. May act as a ATP-Mg/Pi exchanger that mediates the transport of Mg-ATP in exchange for phosphate, catalyzing the net uptake or efflux of adenine nucleotides into or from the mitochondria (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) contains an alternate 5' terminal exon and it thus differs in the 5' UTR and 5' coding region, and it lacks an alternate in-frame exon in the central coding region, compared to variant 1. The encoded isoform (4) has a distinct N-terminus and is shorter than isoform 1.

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