Trpc7 (NM_001302373) Mouse Untagged Clone

CAT#: MC227893

Trpc7 (untagged) - Mouse transient receptor potential cation channel, subfamily C, member 7 (Trpc7), transcript variant 3



  "NM_001302373" in other vectors (1)

CNY 4,560.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms TR; TRP7; Trrp8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC227893 representing NM_001302373
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGTTTGTGGCCCATGCAGTGTCTTTTACCATCTTCTTGGGACTGCTAGTTGTGAACGCCTCCGACC
GGTTTGAGGGCGTTAAGACTCTGCCCAATGAGACCTTCACAGACTACCCCAAACAGATCTTCAGAGTGAA
AACCACACAGTTCTCCTGGACGGAGATGCTCATCATGAAGTGGGTCTTAGGAATGATCTGGTCCGAATGC
AAGGAGATCTGGGAGGAGGGGCCGCGGGAGTACGTGCTACACTTGTGGAACCTTCTGGATTTTGGCATGC
TGTCCATCTTCGTGGCCTCCTTCACTGCGAGGTTCATGGCTTTCCTCAAGGCCAGCGAGGCCCAGCTGTA
CGTGGACCAGTACGTGCAGGATGTAACGCTGCACAACGTCTCACTTCCACCGGAAGTGGCCTACTTCACC
TACGCCAGGGATAAGTGGTGGCCTTCAGACCCCCAGATCATCTCGGAAGGGCTGTACGCCATTGCGGTTG
TCCTCAGCTTCTCCCGGATCGCCTACATCCTGCCAGCCAATGAGAGCTTCGGACCTTTGCAGATCTCCCT
GGGAAGAACTGTGAAAGACATCTTCAAGTTCATGGTCATTTTTATCATGGTATTTGTGGCCTTTATGATC
GGAATGTTCAACCTGTACTCCTACTACCGAGGTGCAAAGTACAACCCAGCGTTTACCACAGTTGAAGAAA
GCTTTAAAACCTTGTTTTGGTCCATATTTGGCTTGTCTGAGGTCATCTCGGTGGTGCTGAAATACGACCA
CAAGTTCATCGAGAATATTGGCTACGTGCTGTATGGGGTTTATAATGTCACCATGGTGGTTGTACTACTC
AACATGCTGATAGCCATGATCAACAACTCCTATCAGGAAATCGAGGAAGACGCGGACGTGGAGTGGAAAT
TTGCTCGAGCGAAGCTCTGGCTTTCTTACTTTGATGAAGGAAGAACTCTCCCTGCTCCCTTTAACCTGGT
GCCGAGCCCTAAATCCTTTTATTATCTCATAATGAGAATCAAGATGTGCCTCATAGAGCTCTGCCAATCT
AAGGCCAAACGCTGTGAAAACGACCTGGAAATGGGCATGCTGAACTCCAAGTTCAGGAAGACTCGCTACC
AGGCTGGCATGAGGAATTCTGAAAACCTGACAGCCAATAGCACCTTCAGCAAGCCCACCAGATACCAGAA
GATCATGAAGCGGCTCATAAAGAGATACGTGCTGAAGGCCCAGGTGGACAGAGAGAACGATGAAGTCAAT
GAAGGTGAACTCAAGGAGATCAAACAAGACATCTCAAGCCTTCGCTATGAGCTCCTGGAGGAGAAGTCTC
AGGCTACAGGAGAGCTGGCAGACCTGATCCAGCAGCTCAGCGAGAAGTTTGGGAAGAATCTGAACAAAGA
CCACCTGCGGGTGAACCAGGGCAAGGACATTTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001302373
Insert Size 1434 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001302373.1, NP_001289302.1
RefSeq Size 3674 bp
RefSeq ORF 1434 bp
Locus ID 26946
Gene Summary The protein encoded by this gene is a member of the transient receptor potential channel family of proteins, which form six-transmembrane cation-permeable channels that are calcium permeant. Knock out mice are viable but display a reduction in the gamma wave activity that precedes seizure induction in response to a muscrarinic agonist, suggesting a functional role for this protein in initiation of seizures. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
Transcript Variant: This variant (3) differs in the 5' UTR and uses a downstream start codon compared to variant 1. It encodes isoform 3, which has a shorter N-terminus compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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