Mst1r (NM_001287261) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CD136; CDw136; Fv; Fv-; Fv-2; Fv2; PTK; PTK8; Ron; ST; STK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227896 representing NM_001287261 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGTGGGTGGTGAGGTCTGCCAACATGAGCTCCGGGGGGATGTGGTGATCTGCCCCCTGCCCCCTT CCCTGCAACTTGGCAAGGATGGTGTCCCATTGCAGGTCTGTGTAGACGGTGGGTGTCACATCCTGAGCCA AGTGGTTCGCTCAAGCCCAGGCAGGGCCTCACAGAGGATACTCCTTATTGCTCTTCTGGTCTTGATCCTG CTTGTGGCTGTGCTGGCCGTTGCCCTGATCTTTAACTCCCGAAGACGGAAAAAGCAGCTAGGTGCTCACT CCCTCTCCCCAACAACACTCTCTGACATCAACGATACAGCTTCCGGGGCTCCGAACCATGAAGAATCGTC AGAGAGTAGGGATGGGACAAGTGTCCCACTGCTGCGGACAGAGTCTATCCGGCTCCAGGATCTGGACAGG ATGCTCCTAGCTGAGGTCAAGGATGTACTGATTCCCCATGAACAAGTGGTCATCCATACTGACCAAGTCA TTGGCAAAGGCCACTTTGGTGTTGTCTACCACGGAGAATATACAGACGGAGCACAGAATCAGACCCACTG TGCCATCAAGTCTCTGAGTCGCATTACAGAGGTGCAGGAGGTGGAGGCTTTCCTGCGGGAGGGGCTGCTC ATGCGTGGCCTACATCACCCAAACATCCTGGCTCTCATCGGTATCATGCTGCCCCCGGAGGGGCTTCCCC GGGTGCTGTTGCCCTATATGCGCCACGGAGACCTGCTTCATTTCATTCGCTCCCCTCAGAGGAACCCCAC TGTGAAGGATCTTGTCAGCTTTGGCCTGCAGGTAGCCTGTGGTATGGAGTACCTGGCAGAGCAGAAGTTC GTGCACAGAGACCTGGCTGCTAGGAACTGCATGCTGGACGAGTCATTCACAGTCAAGGTGGCTGACTTTG GTCTGGCACGGGGCGTCCTAGACAAGGAATACTACAGTGTTCGCCAGCATCGCCATGCTCGCCTGCCAGT CAAATGGATGGCACTGGAGAGCCTGCAGACCTACAGGTTCACCACCAAGTCCGATGTGTGGTCATTCGGG GTGCTGCTCTGGGAGCTACTAACACGGGGTGCTCCACCCTACCCCCATATCGATCCCTTCGACCTCTCTC ACTTCCTGGCTCAGGGCCGTCGCCTGCCTCAGCCTGAGTACTGTCCTGATTCACTGTATCACGTGATGCT TCGATGCTGGGAGGCTGACCCAGCGGCACGACCCACCTTCAGAGCCCTAGTGCTGGAAGTAAAGCAGGTA GTGGCCTCACTGCTTGGGGACCACTATGTGCAGCTGACAGCAGCTTATGTGAACGTAGGCCCCAGAGCGG TGGATGATGGGAGTGTGCCTCCGGAGCAGGTACAGCCCTCGCCTCAGCATTGCAGGAGCACGTCAAAGCC CCGGCCTCTCTCAGAGCCACCCCTGCCCACTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_001287261
Insert Size	1434 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001287261.1, NP_001274190.1
RefSeq Size	1796 bp
RefSeq ORF	1434 bp
Locus ID	19882
Gene Summary	This gene encodes a precursor protein that is proteolytically cleaved to yield an alpha chain and a beta chain which form a membrane-spanning heterodimer. The encoded protein belongs to a family of cell-surface receptor tyrosine kinases involved in signaling from the cell surface to the intracellular environment. The binding of the encoded protein to its ligand, macrophage-stimulating protein, mediates several biological activities including wound healing, tumor immunity, macrophage activation and hematopoiesis as well as cell growth, motility, survival and adhesion. The protein encoded by this gene also functions in early development and the macrophage-mediated inflammatory response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013] Transcript Variant: This variant (2) represents the use of an alternate promoter, differs in the 5' UTR, lacks a significant portion of the 5' coding region and initiates translation at a downstream start codon compared to variant 1. The encoded isoform (2, also known as short-form Ron or SF-Ron or sf-Stk) has a shorter N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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