Micu1 (NM_001291443) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	C730016L05Rik; Calc; Cbara1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227923 representing NM_001291443 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCGTCTTAACACCCTTTCTGCGTTGGCAGAACTAGCTGTGGGCTCTCGCTGGTACCATGGGGCAT CACAGCCCACACAGACCAAACGAAGATTGATGCTGGTGGCGTTCCTGGGAGCATCTGCAGTGACTGCAAG TACCGGTCTCCTGTGGAAGAAGGCTCACGCAGAATCTCCACCATGCGTTAACAGCAAGAAGCCTGACACA GAAGATAAAGAGAGAAACAAAGACAGCGGGGAAGTGTCCAGCCGTGAAGGAAGGGCTGCAGATGCTGCGG CAGAGCCTTATCCTGAGGACAAGAAGAAGAAGCGCTCTGGATTCAGAGACAGAAAAGTGATGGAGTATGA GAATCGAATCCGAGCCTACTCCACACCAGACAAAATTTTCCGATATTTTGCCACCTTGAAAGTAATCAAC GAACCTGGTGAAACCGAAGTGTTCATGACTCCGCAGGACTTTGTGCGCTCCATAACGCCCAATGAGAAGC AGCCAGAACACTTGGGCCTGGATCAGTATATAATAAAGCGCTTTGATGGAAAGGAGTTCTGGCAGAAAAT TGCCCAGGAACGAGAAAAATTTGCTGATGAAGGCAGCATCTTCTACAGCCTTGGAGAGTGTGGGCTCATC TCCTTCTCTGACTACATCTTCCTCACAACAGTCCTCTCCACTCCTCAGAGAAATTTTGAAATTGCTTTCA AGATGTTTGACTTGAATGGAGACGGAGAGGTAGACATGGAGGAGTTTGAGCAGGTTCAGAGCATCATTCG CTCCCAGACCAGCATGGGCATGCGTCACAGAGACCGTCCAACCACTGGGAACACCCTCAAGTCTGGCTTA TGTTCGGCCCTCACGACCTACTTTTTTGGAGCTGATCTCAAAGGGAAACTGACCATCAAAAACTTCCTGG AATTTCAGCGCAAACTTCAGCATGACGTTCTAAAGCTGGAGTTTGAACGCCATGACCCGGTAGATGGGAG AATCTCTGAGAGGCAGTTCGGGGGCATGCTGCTGGCCTACAGTGGAGTGCAGTCCAAGAAGCTGACCGCC ATGCAGAGACAGCTGAAGAAGCACTTCAAAGATGGGAAGGGCCTGACTTTCCAGGAGGTAGAGAACTTCT TCACTTTCCTGAAGAACATTAATGACGTGGACACTGCACTAAGCTTTTACCACATGGCTGGAGCATCTCT TGATAAAGTGACCATGCAGCAAGTGGCCAGGACAGTGGCGAAGGTCGAACTCTCGGACCATGTGTGTGAC GTGGTGTTCGCGCTCTTTGACTGTGATGGCAATGGGGAGCTGAGCAACAAGGAGTTCGTTTCCATCATGA AGCAGCGGCTGATGAGAGGCCTGGAGAAGCCCAAGGACATGGGCTTCACCCGCCTCATGCAGGCCATGTG GAAATGTGCCCAAGAAACTGCCTGGGACTTTGCTCTGCCCAAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291443
Insert Size	1446 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291443.1, NP_001278372.1
RefSeq Size	2370 bp
RefSeq ORF	1446 bp
Locus ID	216001
UniProt ID	Q8VCX5
Gene Summary	Key regulator of mitochondrial calcium uniporter (MCU) that senses calcium level via its EF-hand domains (PubMed:24560927). MICU1 and MICU2 form a disulfide-linked heterodimer that stimulates and inhibits MCU activity, depending on the concentration of calcium (PubMed:24560927). MICU1 acts both as an activator or inhibitor of mitochondrial calcium uptake (By similarity). Acts as a gatekeeper of MCU at low concentration of calcium, preventing channel opening (By similarity). Enhances MCU opening at high calcium concentration, allowing a rapid response of mitochondria to calcium signals generated in the cytoplasm (PubMed:24560927). Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake (By similarity). Induces T-helper 1-mediated autoreactivity, which is accompanied by the release of IFNG (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate exon in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1.

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