Mavs (NM_001206385) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	cardif; D430028G21Rik; IPS-1; Visa
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228071 representing NM_001206385 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACATTTGCTGAGGACAAGACCTATAAGTATATCCGAGACAACCACAGCAAGTTTTGCTGTGTTGACG TTCTGGAGATCCTGCCTTACCTGTCCTGCCTCACAGCTAGTGACCAGGATCGACTGCGGGCTTCCTACAG GCAGATCGGGAACCGGGACACACTCTGGGGACTCTTCAATAATCTCCAGCGCCGGCCTGGCTGGGTGGAG GTCTTCATCCGGGCACTGCAGATCTGTGAGCTGCCTGGGCTGGCTGATCAAGTGACTCGAGTTTATCAGA GCTACCTGCCTCCGGGGACCTCACTCCGCTCCCTAGAGCCACTGCAGTTACCAGACTTTCCTGCTGCGGT TTCTGGACCCTCTGCATTTGCGCCAGGTCACAACATCCCTGACCATGGCTTACGAGAGACACCAAGTTGC CCCAAGCCTGTCCAGGACACCCAGCCACCAGAGTCCCCAGTAGAGAATTCAGAGCAACTCCTCCAGACCA ACTCCGGGGCCGTCGCGAGGATGTCTGGTGGCTCTTTGATACCCTCTCCTAACCAGCAGGCTCTCAGCCC TCAGCCCTCCAGAGAGCATCAAGAGCAAGAACCAGAACTGGGTGGCGCCCACGCAGCAAATGTTGCCTCT GTTCCCATAGCAACCTATGGACCTGTGTCTCCAACCGTTTCCTTCCAGCCCCTTCCACGTACTGCCCTGA GGACAAACCTCTTGTCTGGGGTCACAGTATCAGCCCTATCTGCTGATACCTCTTTGTCCTCCTCGTCCAC TGGATCAGCTTTTGCAAAGGGAGCTGGTGACCAGGCCAAAGCTGCCACCTGTTTCAGTACTACACTCACC AATTCTGTGACTACCAGCTCAGTGCCTTCTCCCAGATTGGTCCCAGTAAAAACCATGTCTTCCAAGTTGC CCCTCAGTTCAAAGTCCACTGCTGCGATGACGTCTACTGTGCTCACCAATACAGCGCCATCAAAATTACC CAGCAACTCAGTGTATGCGGGCACAGTGCCATCCAGAGTGCCTGCTAGTGTGGCCAAAGCACCTGCCAAC ACAATACCACCTGAGAGGAACAGCAAGCAAGCCAAGGAGACCCCGGAGGGTCCAGCAACCAAAGTCACCA CTGGAGGCAACCAGACTGGACCAAATAGCAGTATCAGGAGCTTGCACTCTGGACCAGAGATGAGCAAGCC AGGTGTGCTGGTATCCCAGTTGGACGAGCCATTCTCAGCCTGCTCTGTGGACCTTGCCATTAGCCCTAGC AGCTCCTTGGTCTCAGAACCCAACCATGGTCCAGAGGAGAATGAGTATTCGTCCTTTAGAATCCAGGTAG ACGAAAGCCCCAGTGCTGATCTATTAGGAAGCCCTGAGCCACTAGCCACCCAGCAGCCCCAAGAAGAGGA AGAACATTGTGCCAGTTCAATGCCCTGGGCTAAGTGGCTTGGGGCCACCAGTGCACTCTTGGCTGTATTC CTGGCAGTGATGCTGTACCGTAGTAGGCGCCTGGCCCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001206385
Insert Size	1512 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001206385.1, NP_001193314.1
RefSeq Size	2871 bp
RefSeq ORF	1512 bp
Locus ID	228607
UniProt ID	Q8VCF0
Gene Summary	Required for innate immune defense against viruses (PubMed:24037184). Acts downstream of DHX33, DDX58/RIG-I and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5) (PubMed:24037184). Peroxisomal and mitochondrial MAVS act sequentially to create an antiviral cellular state (By similarity). Upon viral infection, peroxisomal MAVS induces the rapid interferon-independent expression of defense factors that provide short-term protection, whereas mitochondrial MAVS activates an interferon-dependent signaling pathway with delayed kinetics, which amplifies and stabilizes the antiviral response (By similarity). May activate the same pathways following detection of extracellular dsRNA by TLR3 (By similarity). May protect cells from apoptosis (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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