Pigw (NM_001077636) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610044A17Rik; Gwt1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228072 representing NM_001077636 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCAAAAGCAGCTGAAGGAAGCCTTTGTCAGGAACCTCAGTGGGACCAGTGTGCTGGAAGTGACCC AGGGCCTGTGCTTTCCTGCCTTCTGTATCCTGTGTAGAGGCCTTTGGATCATCTTCTCACAGCACGTATG CTCTTTCTCAAACACCTGGAGCACACGATTCCTCATGGACTTTGTTGTCTTGATAGTCCCCCTAGTGATC ACTCTGACTGTATTGTCTTCCTTTATCCTCCTCGAGAATCTCACTGTGATTGTCTGGGGGGCATGGCTGT TGTATCAGATATACCACAGGAGGACTTGCTACGCCAAAGTGCCTGTCCAGAAAGTCTTTGCAAACTTCTT GAAAATCAGCCTAGAATCTGAGTACAATCCCGCAATCACCTGTTACCGGGTCATTAACAGCGTATTTACG GCTATTGCCATTTTGGCCGTGGATTTCCCACTTTTCCCTAGAAGATTTGCCAAAACTGAACTTTATGGGA CAGGAGCAATGGATTTTGGAGTAGGTGGCTTTATTTTTGGGGCGGCAATGGTTTGTCCAGAGGTTAGAAG GAAATCTATAGAAGAGTCTAGATTTAATTATCTTAGAAAATCGCTGTATTCTGTCTGGCCATTGGTCTTC TTAGGAATGGGACGGTTGGTCATTATAAAGTCCATAGGCTACCAGGAACATTCTACCGAGTACGGCATTC ACTGGAACTTTTTCTTTACCATCATAGTTGTGCGATTAGTAACGTCACTGCTTTTGATTATTTTTCCTTT AAATAAGTCGTGGATTGTGGCTGTCAGCATAACTGTGGTATACCAGCTAGCCCTGGATTATACTCCACTC AAGAGGATACTTTTGTATGGCACTGATGGCAGTGGCACAAGGGTTGGCTTCTTAAATGCTAACCGAGAGG GAATAATTTCCACTTTGGGGTATGTGACAATACACATGGCTGGTGTACAAACGGGATTGTATGTGCTTAA GGGAAGAGCACAAGTCAGAGACTGGATAAAAGCTACTTGTTGGGTTTTCTCAGTAGCTGTTGGCTTCTTC ATTTCTCTTCATATAGTGCAAGTAAACATAGAAGCAGTATCTCGAAGAATGGCCAATTTAGCCTTTTGTC TGTGGGTAGTTGCTTCCAGTCTGATGCTTCTTAGTTGTCTGTTACTGAGTGGTATAATTTTGAGTTTTGC CCAATTTCTAATTAAAGGGTCTCTAGTTCCATGTTCTTGGAAACTTATACAGTCTCCTACTACACATAAA AACCATTCTGAATCTCTAATCTTAGAAGCTGAAAAAAACCAACCCAGTCTTTGTTTGATCACAGCTCTGA ACAGAAACCAGCTGTTTTTTTTCTTACTGTCAAATATAACAACTGGTCTGATTAACCTGACGATGGATAC ATTACACACTGGTGCCTTGTGGACCTTAGTTGTGCTCAGTATTTATATGTTTACCAACTGTTTAGTTATA TATGTACTTGATTTACAAGGTAAAACCATAAAGTTTTGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001077636
Insert Size	1512 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001077636.1, NP_001071104.1
RefSeq Size	2258 bp
RefSeq ORF	1512 bp
Locus ID	70325
UniProt ID	Q8C398
Gene Summary	Required for the transport of GPI-anchored proteins to the plasma membrane. Probable acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI-anchor. Acetylation during GPI-anchor biosynthesis is not essential for the subsequent mannosylation and is usually removed soon after the attachment of GPIs to proteins.[UniProtKB/Swiss-Prot Function]

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