Btn2a2 (NM_001289614) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Btn2; D030063K05
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228079 representing NM_001289614 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCTACAACTTCCCTGCGTTCTTGCCCGATAGCCTCCCTTCTCTTCTTCTTGGTCCTCAGCCTGT TTGTGCTGGTCTCAGCCCAGTTTACTGTCATAGGACCAGCTGAGCCCATCCTGGCCATGGTAGGAGAGAA TACCACACTACACTGCCACCTGTCACCAGAGAGAAATGCCGAAGAGATGGAGGTGCGGTGGTTCCGGTGG CGTTTCTTCCCTGCAGTGCTGGTGTACAGAGGCCATCAAGAGAGACCAGAGGAGCAGATGGTGGCATACC GAGGAAGAACCACCTTCATGCGCACAGACATCAGCAAGGGAAGAGTTGCGCTCATTATCCACAATGTCAC AGCCTATGACAATGGCATCTACTGCTGTTACTTCCAGGAAGGCAGGTCCTATGACCAGGCAACCATGAAG CTTATGGTGGCAAGCCTTGGCTCTGAGCCACTTATTAAAATGAAGACACTTGAGGATGGGAGCATCTTGC TAGAGTGCACATCTGAAGGGTGGTACCCAGAGCCCCGAGCTGTGTGGAGAGACCCCTATGATGAAGTTGT ACCTGCCCTGGAGGAGGAGTATACAGCTGACAGAGAAGGCCTCTTCACAGTCACCATGACTATAATCATC AGGGACTGCTCTGTGAGGAACATGACCTGCTCTGTCAATAACACTCTGCTCAGCCAGGAGGTGGAAAGTG TGATTCTCATTCCAGAATCCTTCGTGCCCAGCCTTCCTCTGTGGATGGTGGCTGTGGCTGTCACTCTGCC TGTAGTAATGCTGATTCTCCTCACATCTGGAAGCATCTGCCTTGTCAAGAAACACCGCAGGAAGAAATCT ATTCTGTCAGCTGAAAAAGAAGCCGAATATGAAGAGAAGGAAGCTGCACGGCAACTTCAAGAGGAACTGC CTGACGTGGTCCTGGACCCAGATACAGCTCATCCTGAGCTCTTCCTGTCAGATGACCAGAGAAGTGTAAT ACGAGGCTCTTCGAGGCAGAGTGTGCCTGACAACCCTGAGAGATTTGACTGCCGTCCATGTGTCCTGGGC AGGGAAAGCTTCTCCTCAGGGAAGCATTACTGGGAGGTGGAGGTGGAAAATGTAATGGTGTGGGCCATTG GTGTTTGTAGAGACAGCGTGGAAAGGAAAGGGGAGGCCCTGTTGGTTCCTCAGAATGGCTTCTGGACCCT GGAGATGTTTGGAAGCCAGTATCGAGCCCTGTCCTCCCCAGAAAAGATCATACCTCTGAAAGAGCGTCTT CACCGTATAGCTGTCTTCCTGGACTGTGAGGGTGGAGATATTTCTTTCTACAACATGAGAGACAGATCAC ACATTTACACATGTCCTCCTGTGACTTTCACTGGGCCCCTGAGACCCTTCTTTAGGCTTGGTTCTGATGA CAGTCCCCTGTTCATCTGTCCAGCATTCACAGGGGCACAGGGAGTTACAATACCTGAGGGTGGCTTATTC CTATATAAGACAAGACCAATTTCTCAGAGCCTTGTAAGGAAGCCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289614
Insert Size	1518 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289614.1, NP_001276543.1
RefSeq Size	2956 bp
RefSeq ORF	1518 bp
Locus ID	238555
Gene Summary	Inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an exon in the 3' coding region, compared to variant 1, and encodes a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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