Cttn (NM_001252572) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110020L01Rik; Ems1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228098 representing NM_001252572 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAAAGCCTCTGCAGGCCATGCTGTGTCCATCACGCAGGATGATGGAGGAGCTGATGACTGGGAGA CTGATCCTGATTTTGTGAATGATGTGAGTGAAAAGGAGCAGAGATGGGGTGCTAAAACCGTGCAGGGATC GGGGCACCAGGAACACATCAACATTCACAAGCTTCGAGAGAATGTCTTCCAAGAACACCAGACGCTCAAG GAGAAGGAGCTGGAAACGGGACCCAAGGCTTCCCACGGCTATGGCGGGAAGTTCGGTGTGGAGCAGGATA GGATGGACAGATCAGCCGTGGGCCATGAGTACCAGTCGAAGCTTTCCAAGCACTGCTCACAAGTGGACTC GGTCCGGGGCTTCGGAGGCAAGTTCGGTGTCCAGATGGACAGGGTGGATCAGTCTGCTGTAGGCTTTGAA TACCAGGGGAAGACTGAGAAGCATGCCTCCCAGAAAGACTACTCTAGTGGCTTCGGTGGCAAATACGGTG TGCAAGCTGACCGTGTAGACAAGAGTGCCGTGGGCTTTGACTACCAGGGCAAGACGGAGAAGCATGAGTC TCAGAAAGATTACTCCAAAGGTTTTGGTGGCAAATATGGGATTGACAAGGACAAGGTGGATAAAAGTGCT GTGGGCTTTGAGTATCAAGGCAAGACAGAGAAGCACGAATCCCAGAAAGACTATGTAAAAGGCTTTGGAG GAAAGTTTGGTGTGCAGACAGACAGACAGGACAAGTGTGCCCTTGGCTGGGACCATCAGGAGAAGCTGCA GCTGCATGAATCCCAAAAAGACTATGCCAAAGGATTCGGCGGGAAGTATGGGGTGCAGAAGGATCGGATG GACAAGAATGCATCCACCTTTGAAGAAGTGGTCCAGGTGCCATCTGCCTATCAGAAGACTGTCCCCATTG AGGCCGTAACCAGCAAAACCAGTAATATCCGTGCTAACTTTGAAAACCTGGCAAAGGAGAGAGAGCAGGA GGACAGGCGGAAGGCAGAAGCCGAGAGAGCTCAGCGGATGGCCAAAGAAAGACAGGAGCAGGAGGAGGCG CGCAGGAAGCTGGAAGAGCAAGCCAGAGCCAAGAAGCAGACGCCCCCTGCATCCCCTAGTCCTCAACCAA TTGAAGACAGACCACCCTCCAGCCCCATCTATGAGGATGCAGCTCCGTTCAAGGCCGAGCCGAGCTACCG AGGTAGCGAACCTGAGCCTGAGTACAGCATCGAGGCCGCAGGCATTCCTGAGGCTGGCAGCCAGCAAGGC CTGACCTATACATCAGAGCCCGTGTACGAGACTACAGAGGCTCCTGGCCACTATCAAGCAGAGGATGACA CCTACGATGGGTATGAGAGTGACCTGGGCATCACAGCCATCGCCCTGTATGACTACCAGGCTGCTGGCGA TGATGAGATCTCCTTTGACCCTGATGACATCATCACCAACATAGAAATGATTGACGATGGCTGGTGGCGT GGGGTGTGCAAGGGCAGATACGGGCTCTTCCCAGCCAACTATGTGGAGCTGCGGCAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252572
Insert Size	1530 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252572.1, NP_001239501.1
RefSeq Size	3112 bp
RefSeq ORF	1530 bp
Locus ID	13043
Gene Summary	Contributes to the organization of the actin cytoskeleton and cell shape (PubMed:17403031). Plays a role in the formation of lamellipodia and in cell migration (By similarity). Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density (PubMed:22262902). Plays a role in the invasiveness of cancer cells, and the formation of metastases (By similarity). Plays a role in focal adhesion assembly and turnover (By similarity). In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement (By similarity). Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane (PubMed:17959782). Plays a role in receptor-mediated endocytosis via clathrin-coated pits (By similarity). Required for stabilization of KCNH1 channels at the cell membrane (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate exon in the coding region, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1.

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