Spam1 (NM_001079875) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AV039194; Ph-20
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228112 representing NM_001079875 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGAGTTGAGATTTAAGCACCTCTTTTGGGGGAGCTTTGTTGAATCTGGGGGCACATTCCAAACAG TGTTAATCTTCCTTTTGATTCCATGCTCCTTGACTGTGGATTATAGGGCAGCACCAATTTTATCAAATAC AACTTTCCTTTGGATTTGGAATGTCCCAACTGAACGTTGTGTAGGAAATGTTAATGATCCAATAGATCTG AGCTTCTTCTCTTTAATTGGAAGCCCCCGGAAAACTGCCACAGGGCAACCTGTCACATTATTTTATGTTG ATCGACTTGGTTTGTATCCTCACATAGATGCAAACCAAGCAGAACATTATGGAGGAATACCTCAGAGGGG CGATTATCAAGCTCATTTGCGCAAAGCTAAGACTGACATAGAGCATTACATTCCAGACGACAAATTGGGC TTAGCTATCATTGACTGGGAAGAATGGAGGCCTACCTGGTTGAGAAACTGGAAACCTAAGGATAACTACA GGAATAAGTCTATTGAATTGGTCCAATCAACTAATCCAGGACTTAGTATCACAGAAGCCACCCAGAAAGC CATACAACAATTTGAAGAGGCAGGAAGGAAGTTTATGGAAGGAACTTTACACCTGGGGAAATTCCTTCGA CCAAACCAGCTATGGGGTTATTATCTATTTCCTGATTGTTATAACAATAAGTTTCAAGACCCTAAGTATG ATGGGCAGTGCCCTGCTGTGGAAAAGAAAAGAAATGATAATCTTAAATGGCTATGGAAAGCAAGCACCGG CCTTTACCCATCTGTCTATTTGAAGAAAGACTTGAAGTCCAATCGACAAGCTACCCTCTATGTCCGCTAC CGAGTTGTGGAAGCTATCAGAGTGTCCAAGGTTGGGAATGCATCGGATCCAGTCCCGATTTTTGTCTATA TCCGTCTTGTTTTTACTGATCGTACCTCTGAATACCTTCTAGAGGATGACCTTGTGAATACAATTGGTGA AATTGTTGCTCTGGGTACCTCTGGAATTATAATATGGGATGCTATGAGTTTAGCACAACGTGCGGCAGGT TGCCCAATCCTACATAAATACATGCAGACGACCCTGAATCCATACATAGTCAATGTTACCCTAGCAGCCA AAATGTGCAGCCAAACACTTTGTAATGAGAAAGGCATGTGTTCAAGAAGAAAAGAAAGTTCAGATGTATA TCTTCACTTGAACCCAAGTCATTTTGATATTATGTTAACGGAAACTGGAAAGTACGAAGTTCTTGGCAAC CCCAGGGTTGGAGACTTAGAATACTTTTCTGAACATTTTAAATGCAGCTGTTTTAGCAGAATGACATGTA AGGAGACATCTGATGTAAAAAATGTACAAGACGTGAATGTGTGCGTCGGTGACAATGTTTGTATAAAAGC CAAGGTAGAACCCAACCCAGCCTTCTACCTCCTACCTGGCAAAAGCCTTCTATTTATGACAACACTTGGT CATGTGCTGTACCATCTGCCACAAGATATTTTTGTTTTTCCACGGAAGACACTAGTCAGTACTCCTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001079875
Insert Size	1539 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001079875.2, NP_001073344.1
RefSeq Size	2113 bp
RefSeq ORF	1539 bp
Locus ID	20690
UniProt ID	P48794
Gene Summary	Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.

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