Irx3 (NM_001253822) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI894186
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228149 representing NM_001253822 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTTCCCCCAGCTCGGATACCAGTACATCCGCCCTCTCTACCCACCCGAACGCCCGGGAGCTGCGG GCGGCGGTGGAGGCGGCAGTAGCGCTGGGGGCCGGAGCGGTCCCGGCGCCGGAGCCTCCGAGTTGGCCGC CTCTGGGTCCCTATCCAATGTGCTTTCATCAGTGTACGGGGCACCCTACGCCGCTGCCGCAGCTGCCGCC GCCGCCGCTCAGGGTTACGGCGCCTTCCTGCCCTACGCCACGGAGCTGCCCATCTTCCCACAGCTGGGCG CTCAGTATGAGCTGAAGGACAGCCCTGGCGTCCAGCATCCGGCCACGGCCGCCGCGTTCCCGCACCCGCA CCCCGCCTTCTACCCCTATGGCCAGTACCAGTTCGGGGACCCGTCCCGTCCCAAGAACGCCACCCGGGAA AGCACGAGCACGCTCAAGGCCTGGCTCAACGAGCACCGCAAGAACCCGTACCCCACCAAGGGCGAGAAGA TCATGTTGGCCATCATCACCAAGATGACCCTCACCCAGGTGTCCACCTGGTTCGCCAACGCGCGCCGGCG CCTCAAGAAGGAGAACAAGATGACGTGGGCTCCCCGTAGTCGCACGGACGAGGAGGGCAATGCTTATGGG AGCGAGCGGGAGGAGGAGGACGAAGAGGAAGACGAGGAAGAGAGCAAACGCGAGCTGGAGATGGAGGAGG AGGAGCTCGCAGGAGAGGAGGAGGACACGGGGGGCGAGGGGCTGGCCGACGACGACGAGGATGAGGAGAT CGATTTGGAAAACTTAGACAGCGCGGCAGCCGGGTCGGAGCTGACCCTGGCTGGGGCGGCGCACAGGAAC GGCGACTTCGGCCTGGGACCCATTTCGGACTGCAAAACTAGCGACTCGGACGACAGCTCCGAAGGCTTGG AGGACCGACCACTGTCGGTCCTGAGCCTGGCCCCACCGCCACCGCCCGTGGCCAGGGCTCCTGCATCTCC ACCTTCTCCACCCTCCAGCCTGGATCCCTGCGCTCCGGCCCCGGCGCCCTCCTCCGCCCTCCAGAAGCCC AAGATCTGGTCACTGGCCGAGACGGCCACCAGCCCGGACAACCCACGCCGCTCGCCTCCCGGAGCCGGAG GTTCGCCTCCCGGCGCAGCCGTTGCGCCCCCGACGCTGCAGCTCTCGCCCGCGGCCGCTGCAGCGGCTGC AGCGGCACACAGACTCGTGTCGGCGCCGCTTGGCAAATTCCCCGCTTGGACCAACAGGCCTTTCCCAGGG CCGCCGGCCGGTCCGCGGCCGCACCCGCTGTCCATGCTGGGCTCGGCCCCACAGCACCTGCTGGGACTTC CCGGAGCCGCAGGTCATCCCGCTGCGGCCGCAGCCGCCTATGCTCGGCCTGCCGAGCCGGAGAGTGGAAC AGGTGACAAGAAAAATATCGCTGTAGTGCCTTGGAAGTGGAGAAAAAGTTACTCAAGACAGCTTTCCAGC CGGTGCCAAGGCGGCCACAGAACCATCTGGACGCTGCTCTGGTCTTATCAGCTCTCTCCTCGTCTTAACT TTTCAACCATTTTTAAATCGTTGTACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253822
Insert Size	1569 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253822.1, NP_001240751.1
RefSeq Size	2363 bp
RefSeq ORF	1569 bp
Locus ID	16373
UniProt ID	P81067
Gene Summary	Transcription factor involved in SHH-dependent neural patterning (PubMed:10830170, PubMed:15201216). Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube (PubMed:10830170, PubMed:15201216). Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals (PubMed:10830170, PubMed:15201216). Involved in the transcriptional repression of MNX1 in non-motor neuron cells (PubMed:15201216). Acts as a regulator of energy metabolism (PubMed:24646999).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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