Hnf1b (NM_001291268) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	HNF-1-beta; HNF-1B; HNF-1Beta; Hnf1beta; LFB3; Tcf-2; Tcf2; vHNF1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228202 representing NM_001291268 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTCCAAGCTCACGTCGCTCCAGCAAGAACTCCTGAGTGCCCTGCTGAGCTCCGGAGTCACCAAGG AAGTGCTGATCCAGGCCTTGGAGGAGTTACTGCCGTCCCCGAATTTCGGGGTGAAGCTGGAGACACTGCC CCTGTCCCCCGGGAGCGGGGCGGATCTCGACACCAAGCCGGTTTTCCATACTCTCACCAATGGCCACGCC AAGGGCCGCTTGTCTGGGGACGAGGGCTCAGAGGACGGCGACGACTATGACACTCCTCCCATCCTCAAAG AGCTCCAGGCGCTCAACACCGAGGAGGCCGCGGAGCAGCGGGCCGAGGTGGACCGGATGCTCAGCGAGGA CCCGTGGAGGGCTGCCAAAATGATCAAGGGATACATGCAACAGCACAATATCCCCCAGAGGGAGGTGGTC GATGTCACAGGCCTGAACCAATCCCACCTCTCTCAACACCTCAACAAGGGCACCCCCATGAAGACCCAGA AGAGAGCTGCCCTGTACACTTGGTACGTCAGAAAGCAACGGGAGATCCTCCGACAGTTCAGTCAACAGAA CCAGGGGCCTGGGCAGTCGGAGGACACCTGCTCCGAGCCCACCAACAAGAAGATGCGCCGCAACCGGTTT AAATGGGGGCCCGCATCCCAGCAAATTTTGTACCAGGCCTACGACCGGCAAAAGAATCCCAGCAAGGAAG AGAGGGAGGCCTTAGTGGAGGAGTGTAACAGGGCAGAATGTTTGCAACGAGGGGTCTCCCCTTCCAAAGC CCACGGCCTAGGCTCCAACTTGGTCACGGAGGTCCGTGTCTACAACTGGTTCGCAAACCGCCGGAAGGAA GAGGCGTTCAGGCAGAAGCTGGCCATGGATGCCTATAGCTCCAACCAGACGCACAACCTGAACCCCCTGC TCACCCATGGCTCCCCTCACCATCAGCCAAGCTCCTCTCCACCCAACAAGATGTCAGGAGTGCGCTACAA CCAGCCGGGAAACAATGAGGTCACTTCCTCTTCGACAATCAGTCACCATGGTAACAGTGCCATGGTGACC AGCCAGTCGGTTTTACAGCAAGTCTCCCCAGCCAGCCTGGACCCAGGCCACAGTCTCCTCTCACCTGACA GTAAAATGCAGATCACAGTGTCGGGAGGAGGACTGCCTCCCGTCAGCACCTTGACGAATATCCACAGCCT CTCCCACCACAATCCCCAGCAATCTCAGAACCTCATCATGACCCCCCTGTCTGGAGTCATGGCCATTGCA CAGAGCCTCAACACCTCCCAAGCCCAGGGTGTCCCGGTCATTAACAGTGTGGCTAGCAGCCTGGCAGCCC TACAGCCCGTCCAGTTCTCTCAACAGCTGCACAGCCCTCACCAGCAGCCCCTCATGCAGCAGAGCCCAGG CAGTCACATGGCCCAGCAGCCCTTCATGGCCGCGGTGACTCAGCTACAGAACTCCCACATGTATGCACAT AAGCAGGAACCCCCCCAGTATTCCCACACCTCCCGGTTCCCATCTGCAATGGTGGTCACAGATACCAGTA GCATCAACACTCTCACCAGCATGTCTTCCAGTAAACAGTGTCCACTGCAAGCCTGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291268
Insert Size	1599 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291268.1, NP_001278197.1
RefSeq Size	2765 bp
RefSeq ORF	1599 bp
Locus ID	21410
UniProt ID	P27889
Gene Summary	Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate splice site in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.

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