Tcf12 (NM_001253863) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A130037E08Rik; ALF1; bHLHb20; HEB; HEBAlt; HTF-4; HTF4; ME1; REB
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228212 representing NM_001253863 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACTGTGCTTATCCTGTCCCTGGAATGGGCAACAATTCTTTGATGTATTACTACAATGGGAAAACGG TATATGCACCATCCCCAAATTCTGACGATTTCAACCGTGAATCTCCTAGTTACCCATCTCCCAAGCCACC AACCAGTATGTTCGCTAGCACTTTCTTTATGCAAGATGGGACCCACAGTTCTTCTGACCTTTGGAGTTCA TCGAATGGGATGAGCCAGCCTGGTTTTGGTGGAATTCTGGGGACCTCCACATCCCACATGTCTCAGTCCA GTAGTTATGGCAGCCTTCATTCACATGACCGCTTGAGTTATCCTCCACACTCAGTGTCACCAACAGACAT AAACACAAGTCTTCCGCCAATGTCCAGCTTCCACCGTGGTAGTACCAGCAGCTCACCATATGTTGCCGCC TCACATACTCCTCCCATCAATGGATCAGATAGCATCCTAGGAACCAGAGGGAATGCTGCTGGAAGCTCAC AGACGGGTGATGCACTTGGGAAGGCCTTGGCATCTATTTATTCCCCTGACCACACAAGCAGTAGTTTTCC ATCAAACCCATCAACACCAGTGGGATCTCCTTCACCTCTCACAGGTACCAGTCAGTGGCCCAGAGCTGGA GGGCAAGCTCCTTCATCTCCAAGCTATGAAAACTCACTTCACTCCCTGAAAAATCGAGTTGAGCAGCAAC TTCACGAGCATTTGCAAGATGCAATGTCCTTCTTAAAGGATGTCTGTGAGCAGTCTCGAATGGAAGACCG CTTAGACAGGCTGGATGATGCTATCCATGTGCTACGAAACCATGCAGTTGGACCTTCTACCAGTCTGCCT ACTAGCCACAGTGACATACACAGTTTGCTGGGACCATCCCATAATGCATCAATTGGAAACCTCAATTCAA ACTATGGAGGATCCAGCCTTGTTACAAATAGTCGATCAGCTTCGATGGTCGGAACACATCGGGAAGATTC AGTCAGTCTCAATGGCAATCATTCGGTCCTGTCTAGTACTGTTGCTGCCTCAAACACAGAACTGAACCAT AAAACACCAGAAAATTTCAGAGGTGGTGTACAAAATCAGTCTGGAAGTGTTGTTCCAACAGAAATCAAGA CTGAAAACAAAGAAAAAGATGAAAACCTTCATGAACCTCCTTCATCAGATGACATGAAATCAGATGATGA GTCCTCCCAGAAAGACATCAAGGTCTCATCTAGGGGCAGAACAAGCAGTACCAATGAAGACGAGGATCTG AATCCAGAACAGAAAATCGAAAGGGAGAAGGAAAGGCGGATGGCTAACAATGCCAGAGAGCGCCTGCGCG TGCGGGATATTAACGAGGCGTTCAAGGAGCTTGGCCGAATGTGTCAGCTTCATTTGAAGAGTGAAAAACC TCAGACAAAACTTCTCATTCTTCATCAGGCCGTGGCAGTCATCCTTAGTCTAGAACAGCAAGTGAGAGAG AGGAACCTCAACCCCAAAGCAGCCTGCCTTAAGAGAAGAGAAGAAGAAAAAGTCTCTGCTGCGTCAGCAG AGCCGCCCAACACGTTGCCAGGAGCCCATCCTGGGCTTAGTGAGTCTACCAACCCTATGGGTCATCTGTA A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253863
Insert Size	1611 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001253863.1, NP_001240792.1
RefSeq Size	4029 bp
RefSeq ORF	1611 bp
Locus ID	21406
Gene Summary	Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3').[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) has a different 5' end and lacks several exon in the coding region, which results in the use of a downstream start codon, compared to variant 1. The resulting protein (isoform 3) is shorter and has a distinct N-terminus when it is compared to isoform 1.

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