Trip10 (NM_001242391) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI646975; Cip4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228248 representing NM_001242391 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTGGGGTACCGAGTTGTGGGATCAGTTTGAGGTGCTGGAACGCCACACGCAGTGGGGGCTGGATT TGTTGGACAAATACGTGAAGTTCGTGAAAGAACGCGCGGAGGTGGAGCAGGCTTATGCTAAGCAACTCCG GAGCCTGGTGAAAAAGTATCTTCCCAAGAGACCTACCAAAGATGACCCTGAAGTCAAGTTCAGCCAGCAG CAGTCATTTGTCCAGCTTCTCCAGGAGGTCAATGACTTCGCAGGCCAGAGAGAGCTGGTGGCTGAGAGCC TCGGTATCCGAGTGTGTCTGGAGCTGGCTAAGTATTCACAGGAGATGAAGCAGGAGAGGAAGATGCACTT CCAGGAAGGTCGTCGGGCCCAGCAGCAGCTGGAGAATGGCTTCAAACAGCTGGAGAATAGTAAGCGGAAG TTTGAACGAGATTGCCGGGAGGCTGAGAAAGCGGCTCACACTGCTGAGCGCCTAGATCAGGACATTAATG CCACCAAGGCGGATGTGGAGAAGGCCAAGCAGCAAGCTCACCTTCGGAACCACATGGCAGAAGAGAGCAA GAATGAATATGCGGCCCAGCTGCAGCGCTTCAACCGAGACCAGGCTCACTTCTACTTCTCACAGATGCCC CAGATATTCGATAAGCTGCAGGACATGGACGAACGCCGGGCCACCCGCCTGGGGGCCGGGTATGGGCTCT TATCAGAGGCTGAACTGCAGGTGGTTCCCATTATTGGCAAATGCTTGGAGGGCATGAAGGTGGCCGCCGA GTCTGTGGATGCTAAGAACGACTCACAGGTCCTCATCGAATTACACAAGTCCGGGTTTGCCCGCCCAGGG GACTTGGAATTTGAAGACTTTAGCCAAGTTATCAACCGAGTGCCTTCGGACAGCAGCCTGGGCACCCCGG ATGGCAGGCCTGAGCTCCGAGCAGCCTCCAGCCGTAGCCGCGCCAAGCGTTGGCCTTTCGGGAAAAAGAA CAAGACCGTGGCTACCGAAGATTTCAGTCACTTGCCCCCGGAGCAGCAGAGAAAGCGACTTCAGCAACAG CTGGAAGAGCGGAACCGAGAGTTGCAGAAGGAGGAGGACCAGAGGGAGGCCCTGAAGAAGATGAAAGATG TATATGAGAAAACACCACAAATGGGGGACCCTGCCAGCTTAGAGCCCCGCATTGCAGAGACCCTGGGCAA CATTGAGAGGCTGAAGTTGGAAGTGCAGAAGTATGAGGCTTGGTTGGCAGAAGCTGAAAGCCGGGTCCTC AGTAACCGAGGGGACAGCCTAAGCCGTCACGCTAGGCCCCCTGATCCCCCAACTACTGCCCCACCTGATA GCAGCAGTAGCAGCACCAACAGTGGATCCCAGGACAATAAGGAGAGCTCAGAAGAGCCCCCTTCAGAAGG CCAGGACACCCCCATCTATACTGAGTTCGATGAGGACTTTGAGGAGCCTGCATCCCCTATCGGCCAGTGT GTGGCTATCTACCATTTTGAAGGATCCAGCGAGGGAACCGTCTCCATGTCCGAGGGGGAAGACCTCAGCC TGATGGAGGAAGACAAGGGTGATGGATGGACGCGGGTCAGGAGGAAACAGGGAGCTGAGGGCTACGTGCC CACCTCTTACCTCCGAGTCACACTCAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001242391
Insert Size	1641 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001242391.1, NP_001229320.1
RefSeq Size	2287 bp
RefSeq ORF	1641 bp
Locus ID	106628
UniProt ID	Q8CJ53
Gene Summary	Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL (By similarity). Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) lacks an alternate in-frame exon and uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (4) has the same N- and C-termini but lacks two alternate internal segments compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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