Phactr1 (NM_001302636) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9630030F18Rik; Rpel1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228292 representing NM_001302636 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGTTCCGACTCGCTGGTCCCAGGTACGCACACCCCGCCCATCCGGAGGAGGAGTAAGTTTGCCAACC TGGGGAGGATTTTCAAACCCTGGAAATGGAGGAAGAAGAAAAGTGAAAAGTTCAAGCACACGTCAGCAGC TCTGGAAAGGAAGATTTCGATGAGGCAGAGCAGAGAGGAGCTGATCAAGAGAGGGGTCTTGAAGGAGATC TACGATAAAGATGGAGAACTCTCTATATCAAATGAAGATGACTCCCTGGAGAACGGACAGTCCCTGAGCT CCAGCCAACTCTCTTTGCCTGCTCTGTCGGAAATGGAGCCTGTCCCAATGCCCAGGGACCCATGCTCATA TGAGGTGCTCCAAGCTTCAGACATTATGGATGGACCAGTGTCTGAAGAGAGTCCCTCTGCCAGTGAGTCT GGAGTCCTCCTGTCCCAAGATCCTTCAGCCAAACCAGTTCTGTTCCTGCCCCCCAAAAAATCTGCTGCTT TCCCTGGAGACCATGAGGAGACCCCAGTGAAACAGTTGTCCCTTCACAAGCAGCCCCCGGCCCTGCCTCC CAAACCCACTGCCCGGATTGCCAACCACTTAACAGATCCTGGCGCCCCTGTGAAATTGCCTTGTCTGCCA GTGAAACTGTCGCCTCCGCTACCTCCAAAGAAAGTCCTGATCTGTATGCCTGTAGGGGGGCCAGAGCTCA CTCTGGCATCCTATGCAGCCCAGAAGAGCAGCCAGCAGGCCGTGGCCCAGCACCACCACACCGTCCTACC ATCCCAGATGCAGCACCAGCTGCAGTATGGCAGTCACGGCCAGCATCTCCCATCCTCCACCGGCACCTTG CCCATGCACCCCTCAGGCTGCAGGATGATCGACGAGCTGAACAAGACACTCGCCATGACGATGCAGAGGC TGGAAAGCTCCGAGCAACGAGTCCCCTGTTCCACGTCTTACCACAGTTCTGGTTTGCACTCAAGCGACGG CATCACAAAAGCAGGACCCATGGGCCTTCCGGAAATAAGACAAGTGCCAACTGTTGTGATTGAATGTGAT GACAATAAGGAAAATGTGCCTCATGAACCCGACTATGAAGACTCGCCCTGCCTGTATGGCCGGGAGGAGG AGGAAGAGGAGGAAGATGAAGATGATGACGCCTCTTTGTACACCAGCTCACTGGCCATGAAGGTGTGCAG GAAGGACTCCTTAGCCATCAAACTCAGCAACAGGCCCTCTAAGCGAGAGCTAGAAGAAAAGAACATCCTC CCCAGACAGACGGATGAGGAACGGCTGGAGCTCAGACAGCAGATTGGCACCAAGCTCACCAGGCGGCTGA GCCAGAGACCAACTGCAGAGGAACTGGAACAGAGGAACATTTTGAAGCCTCGGAATGAACAAGAAGAACA GGAGGAGAAGCGGGAAATCAAGAGGAGGCTGACTCGCAAGCTCAGCCAAAGGCCCACAGTGGAAGAACTC CGGGAGAGGAAGATCCTTATCCGCTTCAGTGACTACGTGGAAGTGGCAGATGCCCAAGACTATGACCGAA GGGCAGACAAGCCATGGACCCGCCTCACCGCTGCAGACAAAGCTGCCATTCGGAAGGAACTCAATGAGTT TAAAAGTACCGAGATGGAAGTCCACGAGTTAAGTAGACACTTAACCAGGTTTCATCGACCTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302636
Insert Size	1674 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302636.1, NP_001289565.1
RefSeq Size	5321 bp
RefSeq ORF	1674 bp
Locus ID	218194
UniProt ID	Q2M3X8
Gene Summary	Binds actin monomers (G actin) and plays a role in multiple processes including the regulation of actin cytoskeleton dynamics, actin stress fibers formation, cell motility and survival, formation of tubules by endothelial cells, and regulation of PPP1CA activity. Involved in the regulation of cortical neuron migration and dendrite arborization (PubMed:30256902).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) differs in the 5' UTR, lacks exons in the 5' coding region, and uses a downstream start codon compared to variant 2. The resulting isoform (5) has a shorter N-terminus compared to isoform 2.

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