Prc1 (NM_001285998) Mouse Untagged Clone

CAT#: MC228328

Prc1 (untagged) - Mouse protein regulator of cytokinesis 1 (Prc1), transcript variant 3



  "NM_001285998" in other vectors (1)

CNY 5,510.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms D7Ertd348e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228328 representing NM_001285998
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGAGAAGTGAGGTGCTAGCAGATGAGTCTATCACATGCCTGCAGAAAGCTCTAACTCACCTCCGGG
AAATATGGGAACTAATTGGGATTCCAGAGGAGCAGAGGCTACAAAGAACCGAGGTTGTAAAGAAGCATAT
AAAGGATCTTCTGGATAGAATGATTGCTGAAGAGGAGAGCCTGAGGGAAAGGCTGCTCAAAAGCATATCC
ATCTGTCAGAAGGAGCTGAGTACCCTGTGCAGTGAGCTACAAGTGAAGCCATTTCAGGAAGAAAAAGATA
CAACCATCTTGCAACTAGAAAAAGATTTGCGCACTCAAGTAGAATTGATGAGAAAACAGAAAAAGGAGAG
AAAGCAGGAACTGAAGCTACTTCAGGAACAGGAGCAAGAACTCCGTGACATCCTCTGTATGCCGCCCTGT
GATGTGGACAGCACCTCTGTCCCCACCTTAGAAGAGCTGAAGCTATTCCGACAGCGTGTGGCAACGCTGA
GGGAGACAAAGGAATCGAGGCGTGAAGAATTTGTCAACATTAAGAAACAAATCATATTGTGTATGGAAGA
ATTAGAACACTCTCCAGATACAAGCTTTGAAAGAGATGTGGTGTGTGAAGATGAAAGTGCTTTTTGTTTA
TCACTGGAGAACATTGCAACATTACAGAAGTTGCTGAAGCAGCTGGAAATGAAAAAATCACAAAATGAAG
CAGAATGTGAGGGGCTCCGCACTCAAATCCGAGAGCTCTGGGATAGGTTACAAATACCTGAAGAAGAGAG
AGAGCCTGTGGAGGCAATTATGACTGGATCAAAAACCAAAATCAGGAATGCGCTGAAATTAGAAGTGGAT
CGGTTAGAAGAACTGAAAATGCAAAACATAAAGCAAGTGATTGAGAAAATCCGAGTGGAGCTGGCTCAAT
TCTGGGACCAGTGTTTTTATAGCCAGGAACAGAGGCAGGCTTTTGCCCCTTACTATTCTGAGGACTACAC
AGAAAACCTGCTCCATCTTCATGATGCCGAGATTGTACGGTTAAGAAACTACTATGACGTTCACAAGGAG
CTGTTCCAAGGCGTCCAGAAGTGGGAAGAGAGCTGGAAACTTTTCCTAGAGTTTGAGAGAAAAGCTTCAG
ATCCAGGTCGATTTACAAACCGAGGGGGAAATCTTTTAAAAGAAGAAAAGGAACGAGCAAAGCTCCAGAA
AACACTCCCTAAGCTGGAAGAGGAGTTGAAAGCACGGATTGAACAGTGGGAACAGGAGCACTCAACAGCA
TTTGTGGTGAATGGGCAGAAATTCATGGAGTATGTTACAGAACAGTGGGAATTACATCGGCTGGAGAAAG
AGCGAGCCAAACAGGAGAGGCAACTGAAGAACAAGAAACAGACAGAGGCAGAGATGCTGTATGGCAGTAC
TCCCCGGACACCTAGCAAAAGACCAGGACAGACGCCCAAGAAGTCTGGCAAAGTACGCAAGATGAACACT
ACCACCATGTCTAGTGCTACACCCAACAGTAGCATTCGGCCTGTCTTTGGTGGATCAGTGTACCGGTCAC
CTATGTCTCGACTACCCCCTTCTGGCAGCAAGTCAGTTGTCACTTCTCTGTGTTCTGGAAAGAAAACACC
TCGAGCTGCCCAGCTTAGGGCCAACAAGGAGAACTTGGACCTCAATGGCAGCATCCTGAGCGCGAGAACT
TTCAAAGGCTTCCAGATCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001285998
Insert Size 1701 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001285998.1, NP_001272927.1
RefSeq Size 2972 bp
RefSeq ORF 1701 bp
Locus ID 233406
Gene Summary Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) uses an alternate splice junction at the 3' end of a coding exon compared to variant 1, that causes a frameshift. The resulting isoform (3) has a shorter and distinct C-terminus compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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