Aifm1 (NM_001290364) Mouse Untagged Clone

CAT#: MC228455

Aifm1 (untagged) - Mouse apoptosis-inducing factor, mitochondrion-associated 1 (Aifm1), transcript variant 2



  "NM_001290364" in other vectors (1)

CNY 5,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AIF; AIFsh2; Hq; Pdcd8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228455 representing NM_001290364
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCGGTGTGGAGGCCTGGCGGGTGCTTTCAAGCAGAAACTGGTGCCCTTGGTGCGGACGGTGTACG
TCCAGAGGCCGAAACAGAGGAACCGGCTTCCAGTTGTGCAGTGTCATCTCCTAGGATCCCCTTCTAGAAC
ACTAGCGTCTGCAGGTGCTTCTGGGAAAGATGGCAGCAGCCTAGTATACTTCTTAATTGTAGGAGCAACA
GTGACTGGGGCAGGAATTTATTATGCCTACAAAACTATAAAAGAAGACCAAAAAAGATACAATGAAAGAG
TGATGGGATTAGGACTGTCCCCAGAAGAGAAACAGAGAAGAGCCATTGCCTCCGCTACAGAGGGAGGCTC
AGTTCCTCAGATCAGGGCACCAAGTCACGTCCCTTTCCTGCTGATTGGTGGAGGGACTGCTGCTTTTGCA
GCAGCCAGATCCATCCGGGCTCGGGATCCTGGGGCCAGGGTCCTGATTGTATCTGAAGACCCTGAACTGC
CATACATGCGACCTCCTCTTTCAAAAGAATTGTGGTTTTCAGATGATCCAAATGTCACAAAGACACTGCA
ATTCAGACAGTGGAATGGAAAAGAGAGAAGCATATATTTCCAGCCACCTTCTTTCTATGTCTCTGCTCAG
GACCTGCCTAATATTGAGAACGGTGGTGTGGCTGTCCTCACTGGGAAAAAGGTAGTACATCTGGATGTAA
GAGGCAACATGGTGAAACTTAATGATGGCTCTCAGATTACCTTTGAAAAGTGCTTGATTGCAACGGGAGG
CACTCCAAGAAGTCTGTCTGCCATCGATAGGGCTGGAGCAGAGGTGAAGAGTAGAACAACACTTTTCAGG
AAGATTGGAGATTTTAGAGCCTTGGAGAAGATCTCTCGGGAGGTCAAGTCAATTACAGTTATCGGCGGGG
GCTTCCTTGGGAGTGAGCTGGCCTGTGCTCTTGGCAGAAAGTCTCAAGCCTCGGGCATAGAAGTGATCCA
GCTGTTCCCTGAGAAAGGAAATATGGGGAAGATCCTTCCTCAATACCTCAGCAACTGGACCATGGAAAAA
GTCAAACGAGAGGGAGTGAAAGTGATGCCCAATGCAATTGTACAATCAGTTGGAGTCAGCGGTGGCAGGT
TACTCATTAAGCTGAAAGATGGAAGGAAGGTAGAAACTGACCACATAGTGACAGCTGTGGGCCTAGAGCC
CAATGTTGAGTTGGCCAAGACTGGCGGACTGGAAATAGATTCCGATTTTGGTGGCTTCCGGGTAAATGCA
GAACTCCAAGCACGTTCTAACATCTGGGTGGCAGGGGATGCTGCATGCTTCTATGATATAAAGTTGGGTC
GAAGGCGAGTAGAGCATCATGATCATGCTGTTGTGAGTGGAAGACTGGCTGGAGAAAACATGACTGGAGC
CGCTAAGCCATACTGGCATCAGTCAATGTTCTGGAGTGATTTGGGTCCTGATGTCGGCTATGAAGCTATT
GGTCTGGTGGATAGTAGTTTGCCCACAGTTGGTGTTTTTGCAAAAGCAACTGCACAAGACAACCCAAAAT
CTGCCACAGAGCAGTCAGGAACTGGTATCCGTTCGGAGAGTGAGACAGAGTCAGAAGCTTCGGAAATCAC
AATTCCTCCCAGCGCCCCTGCAGTCCCACAGGTCCCTGTTGAAGGGGAGGACTACGGCAAAGGTGTCATC
TTCTACCTCAGGGACAAAGTTGTGGTGGGGATTGTGCTATGGAACGTCTTTAACCGAATGCCAATTGCAA
GGAAGATCATTAAGGACGGTGAGCAACATGAAGATCTCAATGAAGTAGCTAAACTCTTCAACATTCATGA
AGATTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001290364
Insert Size 1827 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001290364.1, NP_001277293.1
RefSeq Size 2237 bp
RefSeq ORF 1827 bp
Locus ID 26926
UniProt ID Q9Z0X1
Gene Summary Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) lacks an exon and contains an alternate exon in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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