Elf1 (NM_001286411) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Elf-1; mElf-1; p70; Sts1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228463 representing NM_001286411 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCTGTTGTCCAACAGAACGACCTAGTGTTTGAATTTGCAAGTAACGGCATGGAGGACGAGCAGC AGCTTGGTGATCCTGCTATATTTCCTGCTGTTATTGTGGAACATGTTCCTGGTGCTGATATTCTCAATAG TTATGCTGGTCTAGCTTGTGTGGAAGAGCCTAATGACATGATTACTGAGAGCTCATTGGATGTTGCTGAA GAAGAAATCATAGACGATGACGATGACGACATCACCCTTACAGTTGAGGCTTCTTGTCATAATGGAGATG AAACAATTGAGACCATTGAGGCTGCCGAGGCACTCCTCAATATAGATTCTCCCAGCCCTCCTGTACTGGA TGAAAAGCAAATCAATAATAATATATTTAGCTCCTCTGAAGATGACATCGTTGCCCCAATCACCCATGTG TCCGTCACATTAGATGGGATTCCTGAAGTGATGGAAACTCAGCAAGTGCAAGAGACAAATGCAGACTCCC CAGGAGCCTCCTCCCCAGAGCAGCGCAAGAGAAAGAAAGGAAGGAAAACAAAACCACCACGGCCAGATTC CCCGACGACTACACCAAATATCTCTGTGAAAAAGAAAAATAAAGATGGGAAGGGAAACACAATTTACCTT TGGGAGTTTTTGCTGGCCCTGCTTCAGGACAAAGCTACCTGTCCTAAGTACATCAAGTGGACGCAGCGAG AAAAGGGCATTTTTAAGCTGGTTGATTCTAAAGCCGTGTCTAGATTGTGGGGGAAGCACAAAAACAAACC TGACATGAACTACGAGACCATGGGGAGAGCACTCAGGTACTATTACCAAAGAGGTATTCTTGCAAAAGTG GAAGGTCAGCGCTTGGTGTATCAGTTTAAAGAAATGCCAAAAGATCTTATCTACATAGATGATGAGGATC CAAGTTCCAGCATAGAGTCTTCGGATCAGTCCTTATCGTCAACAACCGCATCAAGTCGGAATCAAGCAAA CCGATCAAGAGTATCTTCAAGTCCAGGAATTAAAGGAGGGGCCGCTACAATTCTAAAGCCCGGGAATTCT AAAGCTGCAAACCCCAAAGATCCGGTGGAAGTTGGACAACCTTCAGAAGTTCTGAGGACAGTGCAACCCT CACAGGCTCCCTATCCTACCCAGCTCTTCCGAACTGTTCATGTAGTACAGCCAGTACAAGCTGTCCCAGA GGAAGCAACCATAGCCAGCACCATGCAGGAGGAAGCAGCAAATTCTTCAGTTCCAAGTATTAGGACTATA CAGGCTTCAACCCAGGTTCCAGTAGTGGTGTCTCCTGGCAACCAACAGTTACATACAGTAACAGTGCCAC TCACAACGGTTATAGCCAGCATAGACCCATCATCAGGTGCTGGGTCTCAGAAATTCATTTTACAAACCAT TCCATCGTCACAGCCCATGACAGTACTGAAAGAAAATGTCATGCTACAGTCACAGAAGCCAGGCTCTCCT TCAATTGTCCTCAGCCCCACCCAAGTACAGCAGGTCCTCACAAGCAATGTTCAGTCCATTTGCAATGGAG CGGGCAGTGTGGCATCCGCACCATCGTTCAGCGCGACGACACCTGTGGTGACTTTCTCTCGGAGTTCACA GCTGGTCGCACACCCACCGGGCACTGTGATCACTTCTGTTATCAAAGCTCAGGAAACAAAAACTCTTAAG CAAGAGGTGGAGAAAAAGGCCGAAGATGATTTGAATGAAGATGCTGAGAAAAGTGCCCAGCAGCCCCAGC CTTATGTGATGGTGTTATCCAGTTCAAACGGGTTTTCCTCTCAGGTAGCCGTCAAACAGAATGAACTGCT AGAGCCCAACTCTTTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286411
Insert Size	1839 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286411.1, NP_001273340.1
RefSeq Size	3885 bp
RefSeq ORF	1839 bp
Locus ID	13709
UniProt ID	Q60775
Gene Summary	Transcription factor that activates the LYN and BLK promoters.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein (isoform 1).

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