Papss2 (NM_001201470) Mouse Untagged Clone

CAT#: MC228476

Papss2 (untagged) - Mouse 3'-phosphoadenosine 5'-phosphosulfate synthase 2 (Papss2), transcript variant 2



  "NM_001201470" in other vectors (1)

CNY 5,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1810018P12Rik; AI159688; Atpsk2; AtpsU2; bm; Sk2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228476 representing NM_001201470
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGGGGTCAAGAAGCAGAAGACGGACCAGCAAAAATCCACCAATGTGGTCTACCAGGCCCATCATG
TGAGCAGGAACAAGAGAGGACAAGTGGTTGGAACCAGGGGAGGATTCCGAGGATGTACCGTGTGGCTAAC
AGGTCTCTCTGGTGCTGGGAAAACAACCATAAGCTTTGCTTTGGAAGAGTACCTTGTATCTCACGCCATC
CCATGTTACTCTCTGGATGGGGACAATGTCCGTCATGGCCTTAATAAGAACCTGGGATTCTCTGCGGGGG
ACCGAGAAGAGAATATCCGCCGGATCGCGGAGGTGGCCAGGCTCTTTGCCGACGCCGGCCTGGTTTGCAT
CACCAGCTTTATCTCTCCTTTTGCAAAGGATCGTGAGAATGCCCGAAAAATCCACGAATCAGCAGGACTC
CCGTTCTTTGAGATCTTTGTAGATGCGCCTTTAAATATCTGTGAAAGCCGAGACGTAAAAGGACTCTACA
AACGAGCCCGAGCAGGAGAGATTAAAGGGTTTACAGGCATCGATTCTGACTATGAGAAACCTGAAACTCC
AGAGTGTGTGCTGAAGACCAACCTGTCTTCAGTAAGCGACTGTGTGCAACAGGTGGTGGAACTTTTGCAG
GAGCAGAACATTGTACCCCACACCACCATCAAAGGCATCCACGAACTCTTTGTGCCAGAAAACAAAGTCG
ATCAAATCCGAGCTGAGGCAGAGACTCTCCCATCACTACCAATTACCAAGCTGGATCTGCAGTGGGTGCA
GATTCTGAGTGAAGGCTGGGCCACTCCCCTCAAAGGCTTTATGCGGGAGAAGGAGTACTTGCAAACTCTA
CACTTCGACACTCTACTGGACGATGGAGTCATCAACATGAGTATTCCCATTGTATTGCCCGTTTCTGCGG
ATGACAAGGCACGGCTCGAAGGGTGCAGCAAATTTGCCTTGATGTACGAAGGTCGGAGGGTCGCTCTATT
ACAGGACCCTGAATTCTATGAGCATAGGAAAGAGGAGCGCTGTTCTCGTGTGTGGGGAACAGCCACTGCA
AAGCACCCCCATATCAAAATGGTGATGGAAAGTGGGGACTGGCTTGTTGGTGGAGACCTACAGGTGCTAG
AGAGAATAAGGTGGGACGATGGGCTGGACCAATACCGCCTTACGCCTCTGGAGCTCAAACAGAAGTGTAA
AGACATGAATGCTGATGCCGTGTTTGCATTCCAGTTGCGCAATCCTGTCCACAATGGTCATGCCCTCCTG
ATGCAGGACACCCGCCGCAGGCTCCTGGAGAGGGGTTACAAGCACCCAGTCCTCCTGCTCCACCCTCTTG
GGGGCTGGACCAAGGACGATGACGTACCTCTGGAATGGAGGATGAAACAGCATGCAGCTGTACTGGAGGA
AAGGGTCCTGGATCCCAAGTCAACTATTGTTGCCATCTTTCCATCTCCTATGTTATATGCTGGTCCCACA
GAGGTCCAGTGGCATTGCAGATGCCGGATGATTGCAGGAGCCAATTTCTACATTGTGGGTAGGGATCCCG
CAGGAATGCCCCATCCTGAGACAAAGAAAGACCTATATGAACCCACCCACGGGGGCAAGGTCTTGAGTAT
GGCCCCTGGCCTTACCTCTGTGGAAATAATTCCGTTCCGAGTGGCTGCCTACAATAAAATTAAAAAGGCC
ATGGACTTTTATGATCCAGCAAGGCACGAGGAGTTTGACTTCATCTCAGGAACTCGCATGAGGAAGCTCG
CCCGGGAAGGAGAAGATCCCCCAGATGGCTTCATGGCCCCGAAAGCGTGGAAAGTGTTGACAGATTACTA
CAGGTCTCTGGAGAAGACCAACTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001201470
Insert Size 1845 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001201470.1, NP_001188399.1
RefSeq Size 3599 bp
RefSeq ORF 1845 bp
Locus ID 23972
Gene Summary Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. May have an important role in skeletogenesis during postnatal growth.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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