Recql (NM_001204906) Mouse Untagged Clone

CAT#: MC228538

Recql (untagged) - Mouse RecQ protein-like (Recql), transcript variant 2



  "NM_001204906" in other vectors (1)

CNY 6,180.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms RecQ1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228538 representing NM_001204906
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATCCGTCTCAGCTTTGACAGAGGAACTGGAATCCGTAGCCAGTGAGCTTCATGCCATTGACATCC
AGATTCAGGAACTCACAGAGAGGCGGCAAGAGCTCCTTCAGAGAAAGTCAGTCCTGACAGGGAAAATCAA
ACAGTACTTGGAGGACTCTTCGGCTGAGGCGAGCAGCGACTTGGACACATCACCAGCTGCTTGGAATAAA
GAAGATTTTCCATGGTTCGGAAAGGTAAAAGATGTGCTGCAAAATGTTTTTAAACTGCAAAAATTCAGAC
CTCTGCAGCTGGAAACCATCAATGTCACCATGGCTAGAAAGGATATCTTTCTTGTCATGCCCACAGGAGG
AGGGAAGAGCTTATGCTACCAGCTACCTGCGCTGTGCTCAGACGGTTTTACACTTGTGATTTGCCCACTC
ATCTCTCTTATGGAAGATCAGCTGATGGTTTTAAAACAGTTGGGAATTTCAGCTACTATGTTGAATGCTT
CCAGCTCTAAGGAACATGTAAAATGGGTGCACGCTGAAATGGTGAATAAAAACTCCCAGTTAAAGCTGAT
TTATGTAACTCCAGAGAAAATTGCAAAAAGCAAGATGTTCATGTCAAGACTGGAGAAAGCCTATGAAGCC
GGGAGGCTGACGGGAGCGGCAGTGGATGAAGTGCATTGCTGCAGTCAGTGGGGTCATGACTTCAGGCCTG
ATTATAAGGCTCTTGGCATCTTGAAGCGCCAGTTTCCCAACGCCTCACTGATGGGGCTGACTGCAACAGC
AACCAACCATGTTCTGAAGGATGTACAGAAAATCCTGTGTGTGGGAAAGTGTTTGACTTTTACAGCGTCT
TTCAACCGGCCAAATCTTTTTTATGAGGTTCGGCAAAAGCCCTCAAGTGCTGAAGACTTTACTGAGGACA
TTGTCAAGCTAATTAATGGACGGTACAAAGGACAATCAGGAATCATATACTGTTTCTCTCAGAAAGACTC
TGAACAAATTACCATCAGTTTACAGAAGTTGGGAATTCATGCAGGCACTTACCATGCCAATATGGAACCA
GAAGATAAGACCAAGGTTCATACACAGTGGTCAGCCAATGAGCTTCAGGTGGTAGTGGCAACAGTCGCAT
TTGGCATGGGAATTGATAAGCCAGACGTGAGGTTTGTCATCCATCATTCAATGAGCAAATCCATGGAGAA
TTACTATCAAGAGAGCGGGCGTGCCGGTCGCGATGACTCGAGAGCAGACTGTATTCTGTATTACGGCTTT
GGGGACATATTCAGGATCAGCTCGATGGTGGTCATGGAGAACGTGGGGCAGCAGAAGCTGTATGAGATGG
TGTCATACTGCCAGAATGTCAGCAAGTGTCGCCGTGTGTTAATAGCACAGCATTTTGATGAAGTGTGGAA
CGCAGATGCCTGTAACAAAATGTGTGACAACTGCTGTAAAGACGTTTCGTTTGAGAAAAAGAATGTGACA
CAGCACTGCCGGGACCTGATAAAGATTCTGAAGCAGGCTGAAGGCCTGAATGAAAAGCTGACCCCGCTGA
AGCTGATTGATGCTTGGATGGGAAAGGGAGCAGCCAAGTTGAGAGTGGCTGGCGTTGTTGCCCCGGCCCT
TCCCCGGGAAGACCTGGAGAGAATCGTCGCCCACGCCCTCCTGCAGCAATATCTCAAAGAAGACTACAGC
TTCACGGCTTACGCCACCATCTCCTATCTGAAGGTGGGACCCAGAGCTTGTCTTCTCAGCAACGAAGCCC
ATGCTGTCACTATGCAAGTGAAGAAGTCAGCACAGAGCAGCGTTAGGGGTGCACTGTCTGAAGCTCGTCA
GGTGGAACAAGTGGATTCGAAGGGGGAAGAGCAAAGCTCAGACACACCAGACAAGAGCATCAGATCCCAT
TACAGATGGTTGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001204906
Insert Size 1905 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001204906.1, NP_001191835.1
RefSeq Size 3067 bp
RefSeq ORF 1905 bp
Locus ID 19691
Gene Summary Isoform alpha is a DNA helicase that may play a role in the repair of DNA that is damaged by ultraviolet light or other mutagens. Isoform beta may have important roles in the meiotic process. Both isoforms exhibit a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) differs in the 3' UTR and uses an alternate splice site in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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