Recql (NM_001204906) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	RecQ1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228538 representing NM_001204906 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCCGTCTCAGCTTTGACAGAGGAACTGGAATCCGTAGCCAGTGAGCTTCATGCCATTGACATCC AGATTCAGGAACTCACAGAGAGGCGGCAAGAGCTCCTTCAGAGAAAGTCAGTCCTGACAGGGAAAATCAA ACAGTACTTGGAGGACTCTTCGGCTGAGGCGAGCAGCGACTTGGACACATCACCAGCTGCTTGGAATAAA GAAGATTTTCCATGGTTCGGAAAGGTAAAAGATGTGCTGCAAAATGTTTTTAAACTGCAAAAATTCAGAC CTCTGCAGCTGGAAACCATCAATGTCACCATGGCTAGAAAGGATATCTTTCTTGTCATGCCCACAGGAGG AGGGAAGAGCTTATGCTACCAGCTACCTGCGCTGTGCTCAGACGGTTTTACACTTGTGATTTGCCCACTC ATCTCTCTTATGGAAGATCAGCTGATGGTTTTAAAACAGTTGGGAATTTCAGCTACTATGTTGAATGCTT CCAGCTCTAAGGAACATGTAAAATGGGTGCACGCTGAAATGGTGAATAAAAACTCCCAGTTAAAGCTGAT TTATGTAACTCCAGAGAAAATTGCAAAAAGCAAGATGTTCATGTCAAGACTGGAGAAAGCCTATGAAGCC GGGAGGCTGACGGGAGCGGCAGTGGATGAAGTGCATTGCTGCAGTCAGTGGGGTCATGACTTCAGGCCTG ATTATAAGGCTCTTGGCATCTTGAAGCGCCAGTTTCCCAACGCCTCACTGATGGGGCTGACTGCAACAGC AACCAACCATGTTCTGAAGGATGTACAGAAAATCCTGTGTGTGGGAAAGTGTTTGACTTTTACAGCGTCT TTCAACCGGCCAAATCTTTTTTATGAGGTTCGGCAAAAGCCCTCAAGTGCTGAAGACTTTACTGAGGACA TTGTCAAGCTAATTAATGGACGGTACAAAGGACAATCAGGAATCATATACTGTTTCTCTCAGAAAGACTC TGAACAAATTACCATCAGTTTACAGAAGTTGGGAATTCATGCAGGCACTTACCATGCCAATATGGAACCA GAAGATAAGACCAAGGTTCATACACAGTGGTCAGCCAATGAGCTTCAGGTGGTAGTGGCAACAGTCGCAT TTGGCATGGGAATTGATAAGCCAGACGTGAGGTTTGTCATCCATCATTCAATGAGCAAATCCATGGAGAA TTACTATCAAGAGAGCGGGCGTGCCGGTCGCGATGACTCGAGAGCAGACTGTATTCTGTATTACGGCTTT GGGGACATATTCAGGATCAGCTCGATGGTGGTCATGGAGAACGTGGGGCAGCAGAAGCTGTATGAGATGG TGTCATACTGCCAGAATGTCAGCAAGTGTCGCCGTGTGTTAATAGCACAGCATTTTGATGAAGTGTGGAA CGCAGATGCCTGTAACAAAATGTGTGACAACTGCTGTAAAGACGTTTCGTTTGAGAAAAAGAATGTGACA CAGCACTGCCGGGACCTGATAAAGATTCTGAAGCAGGCTGAAGGCCTGAATGAAAAGCTGACCCCGCTGA AGCTGATTGATGCTTGGATGGGAAAGGGAGCAGCCAAGTTGAGAGTGGCTGGCGTTGTTGCCCCGGCCCT TCCCCGGGAAGACCTGGAGAGAATCGTCGCCCACGCCCTCCTGCAGCAATATCTCAAAGAAGACTACAGC TTCACGGCTTACGCCACCATCTCCTATCTGAAGGTGGGACCCAGAGCTTGTCTTCTCAGCAACGAAGCCC ATGCTGTCACTATGCAAGTGAAGAAGTCAGCACAGAGCAGCGTTAGGGGTGCACTGTCTGAAGCTCGTCA GGTGGAACAAGTGGATTCGAAGGGGGAAGAGCAAAGCTCAGACACACCAGACAAGAGCATCAGATCCCAT TACAGATGGTTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001204906
Insert Size	1905 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001204906.1, NP_001191835.1
RefSeq Size	3067 bp
RefSeq ORF	1905 bp
Locus ID	19691
Gene Summary	Isoform alpha is a DNA helicase that may play a role in the repair of DNA that is damaged by ultraviolet light or other mutagens. Isoform beta may have important roles in the meiotic process. Both isoforms exhibit a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 3' UTR and uses an alternate splice site in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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