Atg7 (NM_001253718) Mouse Untagged Clone

CAT#: MC228712

Atg7 (untagged) - Mouse autophagy related 7 (Atg7), transcript variant 2



  "NM_001253718" in other vectors (1)

CNY 6,750.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228712 representing NM_001253718
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGACCCTGGACTGGCCAAGTTGCAGTTCGCCCCCTTTAATAGTGCCCTGGACGTTGGCTTCTGGC
ACGAACTGACCCAGAAGAAGTTGAACGAGTACCGCCTGGACGAGGCACCCAAAGACATCAAGGGCTATTA
CTACAATGGTGACTCTGCTGGTCTGCCCACCCGCTTGACGTTGGAGTTCAGTGCTTTTGACATGAGTGCC
TCCACGCCTGCCCACTGCTGCCCGGCCATGGGAACCCTGCACAACACCAACACACTTGAGGCTTTTAAGA
CAGCAGACAAGAAGCTCCTTCTGGAGCAGTCAGCAAATGAGATCTGGGAAGCCATAAAGTCAGGTGCTGC
TCTCGAAAACCCCATGCTCCTCAACAAGTTTCTGCTCCTGACCTTCGCGGACCTAAAGAAGTACCACTTC
TACTACTGGTTTTGCTGCCCCGCCCTCTGTCTTCCTGAGAGCATCCCTCTAATCCGGGGACCTGTGAGCT
TGGATCAAAGGCTTTCACCAAAACAGATCCAGGCCCTGGAGCATGCCTATGATGATCTGTGTCGAGCCGA
AGGCGTCACGGCCCTGCCCTACTTCTTATTCAAGTACGATGACGACACTGTTCTGGTCTCCTTGCTCAAA
CACTACAGTGATTTCTTCCAAGGTCAAAGGACAAAGATAACAGTTGGTGTGTACGATCCCTGTAACCTAG
CCCAGTACCCTGGATGGCCTTTGAGGAATTTTTTGGTCCTGGCAGCCCACAGATGGAGCGGCAGTTTCCA
GTCCGTTGAAGTCCTCTGCTTTCGGGACCGCACCATGCAGGGAGCTAGAGACGTGACACATAGCATCATC
TTTGAAGTGAAACTTCCAGAAATGGCATTTAGCCCAGATTGTCCTAAAGCTGTTGGCTGGGAGAAGAACC
AGAAAGGAGGCATGGGTCCGAGGATGGTGAACCTCAGTGGATGTATGGACCCCAAAAGGCTGGCTGAGTC
ATCTGTGGATCTGAATCTCAAGCTGATGTGCTGGCGATTGGTCCCCACCTTGGACTTGGACAAGGTCGTG
TCTGTCAAGTGCCTGCTGCTGGGAGCTGGTACCTTGGGGTGTAATGTGGCTAGGACACTGATGGGCTGGG
GCGTCAGACATGTCACCTTTGTGGATAACGCCAAGATCTCCTACTCCAATCCCGTGAGGCAGCCTCTGTA
TGAATTTGAAGATTGTCTAGGGGGTGGCAAGCCCAAGGCCCTGGCTGCAGCAGAGCGGCTACAGAAAATA
TTTCCCGGAGTGAATGCCAGAGGGTTCAACATGAGCATCCCCATGCCAGGACACCCTGTGAACTTCTCTG
ACGTCACGATGGAGCAGGCCCGCAGAGATGTGGAGCAGCTGGAGCAGCTCATTGATAACCATGATGTCAT
CTTCCTGCTAATGGACACCAGGGAGAGCCGGTGGCTTCCTACTGTTATTGCAGCCAGCAAGCGAAAGCTG
GTCATCAACGCTGCCTTGGGGTTTGATACCTTTGTTGTCATGAGACATGGCCTGAAGAAACCCAAGCAGC
AGGGAGCCGGAGACCTCTGCCCAAGCCATCTTGTAGCACCTGCTGACCTGGGCTCCTCACTTTTTGCCAA
CATCCCTGGATACAAGCTTGGCTGCTACTTCTGCAATGATGTGGTGGCTCCAGGAGATTCAACCAGAGAC
CGGACTCTGGACCAGCAGTGCACAGTGAGCCGCCCAGGCCTGGCCGTGATTGCAGGTGCCCTGGCTGTGG
AGCTGATGGTCTCTGTCCTGCAGCATCCTGAGGGGGGCTACGCCATCGCCAGCAGCAGTGATGACCGCAT
GAATGAGCCTCCCACCTCGCTGGGACTTGTGCCTCACCAGATCCGGGGTTTTCTGTCACGGTTCGATAAT
GTTCTTCCTGTCAGCCTGGCATTTGATAAATGTACAGCCTGTTCACCCAAAGTTCTTGATCAGTACGAGC
GAGAAGGATTCACCTTCCTAGCGAAGGTTTTTAACTCCTCACATTCCTTCTTAGAAGACTTGACCGGTCT
TACCCTGCTCCATCAAGAGACCCAAGCTGCTGAGATCTGGGACATGAGTGACGAGGAGACTGTCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001253718
Insert Size 2097 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001253718.1, NP_001240647.1
RefSeq Size 3895 bp
RefSeq ORF 2097 bp
Locus ID 74244
UniProt ID Q9D906
Gene Summary This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate exon in the 5' coding region, resulting in initiation of translation from a downstream start codon, compared to variant 1. Variants 2 and 3 encode the same isoform (2), which has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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