Kif3a (NM_001290805) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Kif3; Kifl; Kns3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228714 representing NM_001290805 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGATCAATAAGTCGGAGAAGCCGGAAAGCTGCGATAATGTGAAGGTGGTGGTTAGGTGCCGGCCTC TCAATGAGAGAGAGAAGTCAATGTGCTATAGACAGGCCGTCAGCGTGGATGAAATGAGGGGAACTATCAC CGTCCATAAGACCGATTCCTCCAACGAACCTCCAAAGACATTTACTTTCGATACTGTTTTTGGACCAGAA AGTAAACAACTTGATGTGTATAACTTAACTGCAAGACCAATTATTGATTCTGTTCTGGAAGGCTACAATG GGACCATCTTTGCATATGGACAGACTGGGACAGGCAAGACTTTCACCATGGAAGGCGTTCGAGCAGTACC GGGGCTCAGAGGGGTCATCCCCAACTCATTTGCTCACATATTTGGTCACATCGCAAAAGCAGAGGGCGAC ACAAGGTTTTTGGTTCGAGTGTCATACTTGGAGATATATAATGAAGAAGTTCGAGACCTTTTGGGTAAAG ATCAGACCCAGAGGTTAGAGGTTAAAGAAAGGCCTGATGTGGGAGTATATATCAAAGACTTATCAGCATA TGTAGTCAACAACGCTGACGACATGGATAGAATCATGACTCTAGGCCACAAAAACCGTTCTGTCGGTGCG ACTAATATGAACGAGCACAGCTCCCGATCCCACGCCATCTTTACAATTACTATAGAGTGCAGTGAGAAAG GCGTGGACGGGAACATGCACGTCAGGATGGGCAAGCTCCACCTTGTGGATCTGGCTGGGTCAGAAAGACA AGCAAAAACTGGAGCCACAGGACAGCGCCTCAAGGAAGCTACCAAAATCAACCTTTCATTGTCTACCCTG GGCAACGTTATTTCTGCCTTGGTGGATGGAAAAAGCACTCACGTGCCGTATCGGAACTCCAAACTGACCC GTCTCCTGCAGGACTCCTTAGGAGGGAACTCAAAAACCATGATGTGTGCAAATATTGGGCCAGCAGATTA TAATTATGATGAAACTATCAGCACCTTGCGGTATGCCAACCGCGCCAAGAACATTAAAAACAAAGCGAGA ATCAATGAGGACCCAAAGGATGCTCTGCTCCGCCAGTTTCAGAAAGAAATCGAAGAGCTGAAGAAAAAGC TTGAGGAAGGGGAAGAAGTGTCAGGCTCTGACATCAGTGGGTCAGAGGAGGATGACGAGGAGGGTGAACT TGGAGAAGATGGAGAAAAAAAGAAAAAGAGAAGGGGTAAAAAGAAAGTTTCCCCAGATAAGATGGTGGAA ATGCAAGCGAAAATTGACGAGGAGAGAAAAGCACTGGAAACCAAACTTGACATGGAAGAAGAAGAAAGAA ACAAGGCTAGAGCTGAACTAGAGAGACGGGAGAAGGACCTTCTGAAAGCCCAACAAGAGCATCAGTCTTT GCTGGAAAAGCTGTCTGCTTTGGAAAAGAAGGTCATTGTCGGCGGCGTGGACCTGCTGGCCAAGGCTGAG GAGCAAGAGAAGCTTCTGGAGGAGTCCAACATGGAGCTGGAGGAGAGGAGGAGACGAGCTGAGCAGCTTC GGAAAGAGCTGGAGGAAAAGGAGCAAGAACGCTTGGACATCGAGGAAAAATATACCAGTCTGCAAGAGGA GGCACAAGGAAAGACCAAGAAATTAAAGAAAGTCTGGACCATGCTGATGGCTGCAAAGTCAGAGATGGCT GATCTCCAGCAGGAGCATCAGAGGGAGATCGAAGGCCTCCTGGAGAACATCCGGCAGCTCAGCCGGGAGC TCCGTCTTCAGATGCTCATCATTGATAACTTCATACCTCAGGATTACCAGGAAATGATTGAAAACTACGT CCACTGGAATGAAGACATAGGAGAATGGCAGCTAAAATGTGTGGCCTACACAGGAAATAACATGAGGAAA CAAACTCCAGTCCCTGACAAGAAGGAGAGAGACCCATTTGAAGTCGACCTTTCCCACGTGTACCTCGCCT ATACAGAGGAGAGTCTGCGTCAGTCTCTGATGAAGTTAGAAAGGCCACGGACCTCCAAGGGGAAAGCAAG GCCAAAGATGGGGCGAAGAAAGCGTTCTGCAAAGCCTGAGACCGTAATTGATTCTTTACTTCAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290805
Insert Size	2097 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290805.1, NP_001277734.1
RefSeq Size	5441 bp
RefSeq ORF	2097 bp
Locus ID	16568
UniProt ID	P28741
Gene Summary	Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation (PubMed:21670265). Plays a role in centriole cohesion and subdistal appendage organization and function. Regulates the formation of the subdistal appendage via recruitement of DCTN1 to the centriole. Also required for ciliary basal feet formation and microtubule anchoring to mother centriole (PubMed:23386061).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an exon in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.

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