Mapk7 (NM_001291037) Mouse Untagged Clone

CAT#: MC228722

Mapk7 (untagged) - Mouse mitogen-activated protein kinase 7 (Mapk7), transcript variant 6



  "NM_001291037" in other vectors (1)

CNY 6,750.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms BMK-1; BMK1; ERK-5; ERK5; Erk5-T; PRKM7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228722 representing NM_001291037
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCTTTTCCTGCTCCCCAGGCCAGCAGGTGGCCATCAAGAAGATACCTAATGCTTTTGATGTGGTGA
CCAATGCCAAACGGACCCTCAGGGAGCTGAAGATCCTCAAACACTTCAAACACGACAATATCATCGCCAT
CAAGGACATCCTGAAGCCTACTGTGCCCTATGGAGAATTCAGATCTGTCTATGTGGTACTGGACCTCATG
GAGAGCGACCTACACCAGATCATTCACTCTTCACAGCCGCTCACCCTGGAACATGTGAGATACTTCCTGT
ACCAGCTGCTTCGGGGCCTCAAATACATGCACTCTGCTCAGGTCATCCACCGTGATCTTAAACCCTCTAA
CCTTCTGGTCAATGAGAACTGTGAGCTCAAGATCGGTGACTTTGGAATGGCCCGTGGCCTCTGTACTTCC
CCTGCCGAGCACCAGTACTTCATGACTGAGTATGTGGCTACTCGCTGGTACCGTGCCCCGGAGCTCATGC
TTTCCCTGCACGAGTATACGCAGGCAATCGACCTCTGGTCTGTGGGCTGCATCTTTGGTGAGATGCTGGC
TCGGCGCCAGCTCTTCCCAGGCAAAAACTACGTGCACCAGTTACAGCTGATCATGATGGTGTTGGGAACT
CCGTCACCAGCTGTGATTCAGGCTGTGGGGGCTGAAAGGGTGCGAGCCTATATCCAGAGCCTGCCACCAA
GGCAACCTGTGCCTTGGGAGACAGTATACCCAGGTGCTGACCGCCAGGCCCTCTCCCTGCTGGGACGCAT
GTTGCGATTTGAACCCAGTGCCCGAATCTCAGCTGCTGCTGCCCTTCGCCACCCCTTCCTGGCTAAGTAC
CATGACCCTGATGATGAGCCTGATTGCGCCCCACCTTTTGACTTTGCTTTTGACCGTGAAGCCCTTACCA
GGGAGCGCATTAAGGAGGCCATTGTGGCTGAGATTGAGGACTTCCATGCACGACGGGAGGGCATCCGCCA
ACAAATCCGCTTCCAGCCTTCTCTGCAGCCTGTGGCTAGTGAGCCTGTGTGTCCAGATGTTGAGATGCCC
AGTCCCTGGGCTCCAAGTGGAGACTGTGCCATGGAGTCGCCTCCTCCAGCACTGCCACCATGCTCTGATC
CTGCACCTGACACCGTTGATCTGACTCTGCAGCCTGCCCCGCCGGCCAATGGGCCCAGTGCACCCTTGGA
GGCGCCTGAGCCTCGAAAGCCCGTGACAGCTCAGGAACGCCAGCGAGAACGAGAAGAGAAGCGCAGGAGG
CGACAAGAGAGAGCCAAGGAGCGGGAGAAGCGACGACAAGAGAGAGAACGCAAGGAGAGGGGGGCTGGTA
CCTTGGGGGGCCCCTCTACTGACCCTCTGGCTGGACTGGTGCTCAGTGACAATGACCGAAGCCTGCTAGA
GCGGTGGACTCGCATGGCTAGGCCTCCTGCCCCTGCCCCTGCCCCAGCGCCAGCACCAGCGCCAGCACCG
TCCTCTGCCCAGCCCACTAGTACTCCTACTGGCCCCGTATCTCAGTCTACTGGTCCTCTACAGCCTGCAG
GCTCTATTCCGGGTCCTGCCTCCCAGCCTGTTTGCCCACCCCCTGGCCCTGTTCCCCAGCCTGCTGGCCC
TATCCCTGCTCCGCTCCAGACTGCCCCTTCCACTAGCCTTTTGGCCTCCCAGTCACTTGTGCCACCTAGT
GGGTTGCCTGGTTCTGGTGCCCCAGAAGTTCTGCCTTACTTCCCATCTGGCCCACCACCTCCAGATCCTG
GGCTCACCCCTCAGCCTTCTACATCAGAGTCACCTGATGTCAACCTGGTGACTCAGCAGCTGTCCAAGTC
TCAGGTGGAGGACCCCCTGCCTCCTGTGTTCTCTGGCACTCCAAAGGGCAGTGGGGCTGGCTACGGAGTT
GGCTTTGATCTGGAGGAATTCTTAAATCAATCTTTTGATATGGGTGTGGCTGATGGGCCACAGGATGGCC
AGGCAGACTCAGCCTCACTCTCAGCCTCTCTCCTTGCTGACTGGCTTGAGGGCCATGGCATGAACCCTGC
TGACATTGAGTCTCTGCAGCGTGAGATCCAGATGGACTCCCCAATGCTGCTGTCTGACCTGCCTGACCTC
CAAGAGCCCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001291037
Insert Size 2112 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001291037.1, NP_001277966.1
RefSeq Size 3264 bp
RefSeq ORF 2112 bp
Locus ID 23939
UniProt ID Q9WVS8
Gene Summary Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression (By similarity). Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (6) differs in its 5' UTR, uses an alternate start codon, and uses an alternate splice site in the central coding region, compared to variant 1. The encoded isoform (d) is shorter and has a distinct N-terminus, compared to isoform a.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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