Rbak (NM_001045482) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228742 representing NM_001045482 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAGATCAAAGGGCCCACTGTCATTCAAGGATGTGGCTGTGGCCTTCTCCCAGGAGGAGTGGCAGC AGCTGGACCCTGAGGAGAGGACCACATACAGGGATGTGATGCTGGAGACCTACAGCAACCTCGTCTCCGT GGGGTATGACGTTACCAAGCCAAACATGATTATTAAGTTGGAGCAGGGAGAAGAGCCCTGGACAGTGGAA GGTGACCGCCATGCTCAGAGGCATTTGGAAATCAGTAAAGTTTATGACCCCAGAGAAGGAATTGAGGAAA TTGGCGAGAAACATCTGCAGTGCGATGATGATCCCTACTGCTGGAGAGCTGAGAAGGGGGCTGCTTTTGA TGAAGCCTACACCCTCGAAACAGCCCTCATTTCCCCCAGCTCAGGTGCTCACAGCTGTGTCTCCTGTGGG GAGACCCTGGAGTCTGTTTCAGAACTGATTAGCAGTGACGGAAGCTATGCACTGGAGAAGCCCAGCATGT GTTTTGAGTGTGGGAAAGCATACGGAGAAAGCCTTGAAGACTTCAACCAAGATGAGGGTAACTCCTCTCA ACACGATGAAAATATTTTGCAGAAAGTCACCATTTTGGAGAAACCCTTTGCTTATGAGTGCATGGAAGCC TTAGACAGTGAAAGTGTTTTCATGGCTCGTGAGAGAGCTTACATGGGGGAGAAGCCCTATGACTGGGGAG ACTCTGGACCAGACTTCATCCAGATGTCGGACTTCAGTACATATCCGAGATCACAAATGGAACTGAAGCC CTTCGAATGTACACAGTGTGGGAAGTCCTTCTGTAAGAAGTCCAAATTCATCATCCACCAGAGAGCTCAC ACAGGAGAGAAGCCGTACGCTTGTAGTGTGTGTGGAAAGTCCTTCAGTCAAAAGGGAACCCTCACTGTAC ATCGGAGATCGCACTTAGAGGAGAAGCCCTATAAGTGTAATGAGTGTGGGAAGACCTTTTGTCAGAAGTT ACACCTCACACAGCACCAGAGGACTCACTCAGGAGAGAAGCCCTATGAATGTAGCGAGTGCGGGAAGTCC TTCTGCCAGAAGACACACCTCACTCTCCACCAGAGGAATCACTCAGGCGAGAGGCCCTACCCGTGTAATG AATGTGGGAAATCCTTCTCCCGGAAGTCTGCCCTCAATGACCATCAGAGGACACACACAGGAGAGAAGCT CTATAAGTGTAATGAGTGTGGGAAATCCTACTACCGGAAGTCCACCCTCATTACACACCAGAGGACACAC ACAGGGGAGAAGCCCTATCAGTGTAGTGAGTGTGGGAAATTCTTCTCTCGGGTGTCGTACCTCACCATAC ACTATCGGAGCCATTTAGAAGAGAAACCTTACGAGTGTACAGAATGTGGGAAAACCTTCAATCTAAACTC TGCTTTCATTAGGCACTGGAAGGTCCATGCAGAAGAGAGAGTCCAGGAGTGTGGCGAGTGTGGGAAGCCA TCCCCGCTGCAGTGTGCCCCTGATCACACAGGCGACCTAGGAGAGAAGCGCTATGAGTGTAATGAGTGTG GAAAGACCTTCCTTGACAGCTCGGCCTTCCATAGGCACCAGTCCGTCCCTGAAGGGGAGAAAACTTACGA GTGTAATATATGCGGAAAGTCCTTCTCGGATTCGTCATGCTACACTGTGCACTACAGAGGTCATTCCGAA GAGAAGCCCTTCGGGTGCAGTGAATGTGGGAAGACCTTCTCTCATAACTCATCCCTCTTCAGACATCAAA GGGTCCACACCGGCGAGAAGCCGTACGAGTGTTACGAATGTGGGAAGTTCTTCTCTCAGAAGTCGTATCT CACTATCCATCACCGGATCCATTCGGGAGAGAAGCCCTACGAGTGCAGCAAGTGTGGGAAAGTGTTCTCG CGGATGTCGAACCTCACCGTACACTACCGAAGCCATTCAGGAGAGAAGCCGTATGAGTGCAACGAGTGTG GGAAAGTCTTTTCTCAGAAGTCGTACCTCACCGTGCATTACCGGACTCATTCAGGAGAGAAACCTTATGA ATGTAACGAATGTGGGAAAAAATTCCACCACAGATCAGCCTTCAATAGCCATCAGAGAATTCATAAAAGA GGCACTGTAAATGTACTCACTGTAGAAAAGCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001045482
Insert Size	2136 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001045482.2, NP_001038947.1
RefSeq Size	3380 bp
RefSeq ORF	2136 bp
Locus ID	57782
UniProt ID	Q8BQC8
Gene Summary	May repress E2F-dependent transcription. May promote AR-dependent transcription.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All three variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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