Usp44 (NM_001206851) Mouse Untagged Clone
CAT#: MC228745
Usp44 (untagged) - Mouse ubiquitin specific peptidase 44 (Usp44), transcript variant 1
CNY 6,840.00
货期*
4周
规格
Product images
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经常一起买 (3)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | E430004F17Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC228745 representing NM_001206851
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATAGGTGCAAGCACGTTGAGCAGTTGCAGCTTGCTCAAGGCCATTCCATCTTGGACCCTCAGAAAT GGTACTGTATGGTCTGCAACACAACCGAGTCCATTTGGGCTTGCCTCAGCTGTTCCCACGTCGCCTGTGG GAAGTACATCCAAGAGCACGCACTGAAGCACTTTCAAGAAAGCAGCCATCCCGTTGCCTTCGAGGTGAAC GACATGTACGCTTTTTGTTATCTTTGTAACGATTATGTTCTAAACGATAACGCAGCCGGAGACCTGAAGT CACTACGGAGTACACTAAGTACAATCAAAAGTAAAAAGTACCCCTGTGTGGTTCCGAGTGACTCGGTTTT ACATCCTGTGGATGCACAGGACCGTGTTTACTCCTTACTCGACGGCACCCAATCTCTGCCCGGAAACGAG GATCCAACGTGCGCCGCCCTCTGGCACAGGAGACGGGTGCTCATGGGGAAAGCCTTTCGAACTTGGTTCG AACAGTCAGCAATTGGAAGGAAAGGGCAAGAGCCAACTCAGGAGAGAATGGTAGCAAAACGAGAGGCGAA GAGAAGGCAGCAGCAGGAGTTGGAGCAGCAGATGAAAGCCGAGCTGGAAAGCACACCTCCGCGGAAGAGT TTACGCTTACAAGGCTCCTCGGAGGAGGCGGCCACAATCGAAATCGTGCCAGTGCGGGCACCGCCCCCAC CTCCCGCATCCCCAGCAAAGGACAAAGCCGCGCTACCTACTTCGGAAGACAGGACTTTTAAAAAAGTAAG TGACTCCTTAATCAAACGAAGGCCCATGGTAACTCCTGGTGTAACCGGACTGAGAAACTTAGGAAATACT TGCTATATGAATTCTGTTCTTCAAGTGTTGAGTCATTTACTCATTTTTCGACAATGTTTTTTAAAGCTTG ACCTGAACCAGTGGCTGGCTGTGGCTGCCAGCGATAAGGCCCGATCCTATAAGCACTCAGCCGTCACGGA GGCGGCGGCACAGCAGATGAACGAAGGGCAAGAGAAAGAGAAAGGCTTCGTATGCTCCAGACATTCGGGT TTATCCTCAGGCCTGAGCGGAGGGGCCTCAAAAGGTCGGAACATGGAGCTTATTCAGCCAAGGGAGCCCA GTTCCCCGTACAGTTCTCTCTGCCATGAATTGCATATCCTGTTCCAAGTCATGTGGTCTGGAGAGTGGGC CTTGGTCTCACCGTTTGCCATGCTTCACTCCGTGTGGAGGCTGATCCCTGCTTTCCGCGGTTACGCCCAG CAGGATGCTCAGGAATTTCTTTGTGAACTTCTGGATAAGATACAACGTGAACTAGAGACAACCGGAACCA AGTTACCAGCCCTCATCCCCACTTCCCAAAGGAGACTTATTGAGCAAGTTCTGAATGTGGTGAATAACAT TTTTCACGGACAATTTCTTAGTCAGGTTACATGTCTTGCTTGTGACAACAAATCAGATACCATAGAATCC TTCTGGGACTTGTCCCTGGAGTTTCCAGAGAGATACCAGTGCAGTGGGAAAGATGCTGCCTCCCAGCCGT GCCTGGTTACTGACATGTTGGACAAATTTACAGAAACAGAAGCTTTAGAAGGGAAAATCTACATGTGTGA CCATTGTAACTCAAAGCGTAGAAAGTTTTCGTCAAAATCAGTTGTATTCACAGAAGCCCAGAAGCAGCTT ATGATATGCCATCTACCTCAGGTTCTCAGACTGCACCTCAAACGGTTCAGATGGTCAGGACGTAATAACC GAGAGAAGATTGGTGTTCATGTTGTCTTTGAGGAAACCTTAAACATGGAGCCTTATTGCTGCAGGGAGAC CCTGAACGCTCTCAGACCAGAGTGCTTTCTCTATAATTTATCTGCTGTCGTAATTCACCATGGAAAAGGA TTTGGCTCAGGACACTACACTGCCTACTGCTACAATTCCGAAGGAGGGTTCTGGGTCCACTGCAACGATT CCAAGCTGAGCATGTGCACGATGGAAGAAGTACGCAAGGCCCAAGCTTATATCTTATTTTATACTCAGCG AGTTACTGAGAATGGACATTCGAAACTCTTGCCTCCCGAGCTCCTGTCCAATAGCCAGCACCCCAGTAAG GAGACTGATGCCTCTTCTAATGAAGTCCTTAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001206851 |
Insert Size | 2136 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001206851.1, NP_001193780.1 |
RefSeq Size | 2533 bp |
RefSeq ORF | 2136 bp |
Locus ID | 327799 |
UniProt ID | Q8C2S0 |
Gene Summary | Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination.[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR230956 | Usp44 (myc-DDK-tagged) - Mouse ubiquitin specific peptidase 44 (Usp44), transcript variant 1 |
CNY 7,130.00 |
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