Trim3 (NM_001285873) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	BERP1; HAC1; Rnf22
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228757 representing NM_001285873 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAAGAGGGAGGACAGCCCTGGCCCGGAGGTGCAGCCAATGGACAAGCAGTTTCTGGTATGCAGTA TCTGTCTGGATCGGTACCGGTGCCCCAAAGTTCTGCCTTGTCTACATACCTTCTGTGAAAGATGCCTCCA GAACTACATCCCTCCTCAGAGCCTGACACTGTCCTGTCCAGTGTGCCGGCAGACATCCATCCTCCCAGAA CAGGGCGTCTCAGCCCTACAAAACAACTTCTTCATCAGCAGCCTCATGGAGGCCATGCAGCAGGCACCTG AGGGGGCTCATGACCCTGAAGACCCCCACCCCCTCAGCGCAGTGGCTGGCCGCCCTCTCTCCTGCCCCAA CCATGAAGGCAAGACAATGGAGTTTTACTGTGAGGCCTGTGAGACTGCCATGTGTGGTGAGTGCCGCGCA GGGGAGCACCGTGAACACGGCACAGTGCTGTTGCGGGATGTGGTGGAGCAGCACAAGGCAGCTCTGCAGC GCCAGCTTGAGGCTGTGCGTGGCCGATTGCCACAGCTGTCTGCAGCTATCGCCTTGGTTGGGGGTATCAG CCAGCAGCTGCAAGAGCGCAAGGCAGAGGCCCTGGCTCAGATAAGTGCTGCCTTTGAGGACCTGGAGCAA GCTCTGCAGCAGCGCAAGCAGGCTCTGGTCAGCGACTTGGAGAGCATTTGTGGGGCCAAACAGAAGGTGT TGCAGACACAGTTAGACACACTGCGCCAGGGCCAGGAGCACATTGGCAGTAGCTGCAGCTTTGCAGAGCA GGCACTGAGACTGGGCTCTGCCCCTGAGGTTTTGCTAGTAAGAAAGCATATGCGAGAGAGGCTGGCTGCT CTGGCAGCTCAGGCCTTCCCAGAAAGGCCACATGAGAATGCCCAGCTGGAACTGGTCCTTGAAGTGGACG GGTTGCGGAGATCGGTGCTCAACCTGGGGGCACTGCTCACCACCAGTGCTACAGCCCATGAGACAGTGGC CACGGGCGAGGGCCTGCGCCAGGCACTAGTTGGCCAGCCTGCTTCTCTCACTGTCACCACCAAAGACAAA GATGGGCGGCTGGTGCGCACAGGCAGCGCAGAGCTGTGTGCAGAGATCACTGGCCCCGATGGTGTGCGCC TTGCAGTACCGGTGGTGGACCACAAAAATGGCACATACGAGCTGGTGTACACTGCACGCACAGAAGGTGA CCTGCTCCTCTCAGTGCTGCTCTATGGACAGCCGGTGCGTGGCAGCCCTTTTCGTGTGCGTGCCCTTCGA CCTGGGGACCTGCCACCTTCCCCAGATGATGTGAAGCGCCGGGTCAAGTCTCCCGGTGGTCCTGGCAGTC ATGTGCGCCAGAAGGCAGTTCGTAGGCCGAGCTCCATGTACAGCACTGGTGGCAAACGGAAGGACAATCC AATTGAAGACGAACTCGTCTTTCGTGTTGGTAGTCGTGGAAGGGAGAAGGGTGAATTCACCAATTTACAA GGTGTGTCCGCTGCTAGCAGTGGCCGCATCGTGGTAGCAGACAGCAACAACCAATGTATTCAGGTTTTCT CCAATGAGGGCCAGTTCAAGTTCCGATTTGGGGTCCGTGGGCGCTCACCTGGGCAACTACAGCGTCCTAC AGGTGTGGCAGTGGACACCAATGGAGACATTATTGTGGCAGACTATGACAACCGTTGGGTCAGCATCTTC TCTCCTGAAGGCAAGTTCAAGACCAAGATTGGAGCTGGCCGCCTCATGGGACCCAAGGGAGTGGCTGTGG ACCGAAATGGACATATCATTGTGGTGGATAACAAATCTTGCTGTGTCTTCACCTTCCAGCCCAATGGCAA GCTTGTTGGGCGTTTTGGAGGCCGTGGGGCCACTGACCGCCACTTTGCAGGGCCCCACTTTGTGGCTGTG AACAACAAGAATGAGATTGTAGTAACGGATTTCCATAACCATTCAGTGAAGGTGTACAGTGCTGACGGAG AGTTCCTCTTCAAATTTGGCTCGCATGGCGAGGGCAACGGACAGTTCAATGCCCCCACGGGAGTAGCTGT GGATTCCAATGGGAACATCATCGTGGCCGACTGGGGCAACAGCCGCATACAGGTTTGGAGGATGGAGAGT CCAAGATCAAGGTGCTGGTGTTTTTGGTGCCTGGTGAAAATGGCCTGCTCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285873
Insert Size	2154 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285873.1, NP_001272802.1
RefSeq Size	2529 bp
RefSeq ORF	2154 bp
Locus ID	55992
UniProt ID	Q9R1R2
Gene Summary	Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (By similarity). Positively regulates motility of microtubule-dependent motor protein KIF21B (PubMed:24086586).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 3' UTR and coding region and uses an alternate in-frame splice junction compared to variant 5. The resulting isoform (b) has a shorter and distinct C-terminus and lacks an alternate internal segment compared to isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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