Gsn (NM_001206368) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	ADF
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228803 representing NM_001206368 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGTGGAGCACCCCGAATTCCTGAAGGCAGGGAAGGAGCCTGGCCTGCAGATCTGGCGTGTGGAGA AGTTTGACCTGGTGCCTGTGCCCCCCAACCTCTATGGAGACTTCTTCACGGGTGATGCCTATGTCATCCT AAAGACTGTGCAGCTGAGGAATGGGAATCTGCAGTATGACCTCCACTATTGGCTGGGCAATGAATGCAGC CAGGATGAGAGCGGGGCTGCTGCCATCTTTACTGTGCAACTGGATGACTACCTGAACGGCCGGGCTGTAC AGCACCGTGAGGTTCAGGGCTTTGAGTCGTCCACCTTCTCCGGCTACTTCAAGTCTGGACTTAAGTACAA GAAAGGAGGTGTGGCATCTGGATTCAAACACGTGGTACCCAATGAGGTGGTGGTCCAGAGGCTCTTCCAG GTCAAAGGACGCCGTGTAGTCCGTGCTACTGAGGTACCTGTGTCCTGGGACAGTTTCAACAATGGCGACT GCTTCATTCTGGACCTGGGAAACAATATCTATCAGTGGTGTGGCTCTGGCAGCAACAAATTTGAAAGGCT GAAGGCCACACAGGTGTCCAAGGGCATCCGGGACAACGAGAGGAGTGGCCGTGCTCAAGTACACGTGTCT GAAGAGGGAGGAGAGCCAGAAGCCATGCTGCAGGTGCTGGGCCCCAAGCCAGCTCTGCCTGAAGGTACCG AGGACACAGCCAAGGAAGATGCAGCCAACCGAAGGCTGGCCAAGCTCTACAAGGTCTCCAACGGTGCAGG TAGCATGTCAGTCTCCCTAGTGGCTGATGAGAACCCCTTCGCCCAGGGCGCCCTGAGATCTGAGGACTGC TTCATCCTGGACCATGGCAGAGATGGGAAAATCTTTGTTTGGAAAGGCAAGCAGGCCAACATGGAGGAGC GGAAGGCTGCCCTCAAAACAGCCTCTGACTTCATCTCCAAGATGCAGTACCCCAGGCAGACCCAGGTTTC AGTTCTCCCAGAGGGCGGTGAGACCCCTCTCTTTAAGCAGTTCTTCAAGAACTGGCGGGACCCAGACCAG ACAGACGGCCCCGGCCTGGGCTACCTCTCCAGCCACATTGCCAACGTGGAGCGCGTACCTTTCGATGCTG CTACGCTGCACACCTCCACCGCCATGGCCGCTCAGCACGGCATGGATGATGATGGAACTGGCCAGAAACA GATCTGGAGAATTGAAGGTTCCAACAAGGTGCCAGTGGACCCTGCCACATACGGGCAGTTCTATGGAGGC GACAGCTACATCATTCTGTACAACTACCGCCACGGTGGCCGCCAGGGACAGATCATCTACAACTGGCAGG GTGCCCAGTCTACCCAGGATGAGGTTGCTGCTTCTGCCATCCTGACTGCCCAGCTGGATGAGGAGCTGGG AGGAACTCCTGTCCAGAGCCGAGTGGTCCAAGGCAAAGAGCCTGCACACCTCATGAGCTTGTTTGGCGGG AAGCCCATGATCATCTACAAGGGTGGCACCTCCCGTGATGGTGGGCAGACAGCTCCTGCCAGTATCCGCC TCTTCCAAGTGCGAGCCAGCAGCTCTGGAGCCACCAGGGCTGTGGAGGTGATGCCTAAGTCTGGTGCTCT GAACTCCAACGATGCCTTTGTGCTGAAAACCCCCTCCGCTGCCTACCTGTGGGTGGGCGCAGGAGCCAGT GAGGCGGAGAAGACCGGCGCCCAGGAGCTTCTGAAGGTGCTTCGGTCCCAGCATGTGCAGGTGGAAGAAG GCAGTGAGCCAGATGCCTTCTGGGAGGCTCTGGGCGGGAAGACTGCCTACCGCACATCCCCCAGGCTTAA GGACAAGAAGATGGATGCCCATCCTCCTCGACTCTTTGCCTGCTCCAACAGGATCGGACGCTTTGTGATC GAAGAGGTTCCTGGCGAGCTTATGCAGGAAGACCTGGCTACTGATGACGTCATGCTCCTGGACACCTGGG ACCAGGTCTTTGTCTGGGTTGGAAAAGACTCCCAGGAAGAGGAAAAGACGGAAGCCTTGACTTCTGCTAA GCGGTACATCGAGACAGATCCAGCAAATCGGGACAGGCGGACCCCCATCACAGTCGTTAGGCAGGGCTTT GAGCCTCCTTCCTTCGTGGGCTGGTTCCTCGGCTGGGATGACAACTACTGGTCGGTGGATCCTTTGGACC GGGCCTTGGCTGAGCTGGCTGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001206368
Insert Size	2196 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001206368.1, NP_001193297.1
RefSeq Size	2556 bp
RefSeq ORF	2196 bp
Locus ID	227753
Gene Summary	Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2, 3 and 4 encode the same isoform (2). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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