Sema3b (NM_001291539) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	LUCA; Se; sem; SemA; sema5; Semaa; semaV
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228846 representing NM_001291539 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCGGGCTGAGGCCGCCGCCATGATCCCAGGCCTGGCCCTTCTCTGGGTAGCAGGGCTAGGGGATA CTGCCCCTAACCTTCCCCGCCTTCGGCTCTCCTTTCAAGAATTACAGGCCCGGCATGGTGTCCGAACCTT CAGGCTGGAGCGGACCTGCTGTTATGAAGCCTTGCTGGTGGATGAGGAGCGTGGACGCCTGTTTGTGGGT GCTGAGAACCACGTGGCTTCCCTCAGCCTGGACAACATCAGCAAGCGAGCCAAGAAGCTGGCCTGGCCCG CCCCCGTGGAATGGCGTGAAGAATGCAACTGGGCAGGGAAGGACATTGGTACCGAGTGCATGAACTTCGT GAAGCTGCTGCACACCTACAACCACACCCACTTGCTGGCCTGTGGCACAGGGGCTTTCCACCCAACCTGT GCCTTTGTGGAGGTGGGCCACCGGCTGGAGGAACCCATGCTTCAACTGGACCGGAGGAAACTTGAGGACG GCAAGGGGAAGACTCCTTATGACCCAAGGCATCGGGCTGCCTCGGTGCTGGTGGGGGAAGAACTGTATTC TGGGGTGACAGCAGACCTTATGGGCCGGGACTTTACCATCTTTCGAAGCCTTGGTCAGAATCCGAGTCTC CGAACAGAGCCCCATGATTCCCGCTGGCTCAATGAACCCAAGTTTGTCAAGGTCTTTTGGATCCCAGAGA GTGAGAACCCTGATGACGATAAAATCTATTTCTTCTTCCGCGAGTCCGCTGTGGAAGCAGCACCAGCAAT GGGGCGCATGTCTGTGTCTCGTGTTGGCCAGATCTGCAGGAATGACCTGGGTGGCCAGCGGAGCTTGGTC AACAAATGGACCACATTTCTGAAGGCGCGGCTTGTGTGCTCAGTACCTGGAGTTGAGGGTGACACCCACT TTGACCAACTTCAGGATGTTTTCCTTCTGTCCTCCCGAGACCGCCAGACACCTCTTCTCTATGCTGTCTT CTCCACCTCCAGTGGTGTCTTCCAGGGCTCTGCTGTGTGCGTGTACAGCATGAACGATGTGCGCCGAGCC TTCTTGGGACCTTTTGCTCACAAAGAGGGGCCTACACACCAGTGGGTGTCCTACCAGGGTCGTGTCCCCT ACCCAAGACCTGGCATGTGCCCCAGCAAGACCTTTGGCACCTTCAGCTCCACCAAGGACTTCCCAGATGA CGTTATCCAGTTTGCTCGGAACCACCCTCTCATGTACAACCCAGTCCTGCCCATGGGGGGGCGCCCTCTC TTCCTACAAGTGGGAGCTGGGTACACCTTCACCCAAATCGCCGCAGACCGAGTAGCAGCTGCCGATGGAC ACTACGATGTTCTCTTCATTGGTACAGATGTGGGCACAGTGCTGAAAGTGATCTCAGTCCCCAAAGGCAG CCGACCTAATTCTGAAGGACTTCTCCTGGAAGAGCTGCAGGTGTTCGAGGACTCTGCCGCTATCACCAGC ATGCAAATCTCCTCTAAAAGGCAACAACTCTACATAGCATCGCGCAGCGCAGTGGCCCAGATTGCTTTGC ATCGCTGCACTGCCCTAGGCCGCGCCTGCGCAGAATGCTGCTTGGCCCGTGATCCTTACTGCGCCTGGGA TGGATCAGCTTGCACACGCTTCCAGCCTACGGCCAAGAGACGGTTCCGGAGGCAAGACATAAGGAATGGC GACCCCAGCACCCTATGCTCTGGAGACTCTTCTCACTCTGTGCTGCTGGAGAAGAAGGTGTTGGGTGTGG AGAGCGGCAGCGCGTTTCTGGAGTGTGAGCCCCGCTCGCTCCAGGCGCATGTGCAGTGGACCTTCCAAGG TGCAGGGGAGGCAGCTCACACCCAGGTGCTGGCTGAGGAGAGAGTAGAGCGCACTGCGCGGGGGCTGCTG TTGCGGGGGCTGCGGCGCCAGGACTCTGGCGTGTATCTTTGCGTCGCGGTTGAACAAGGCTTTTCACAAC CACTGCGTCGCCTGGTGCTGCATGTGTTGAGTGCGGCGCAGGCTGAACGACTGGCACGGGCAGAGGAAGC AGCCGCTCCTGCACCTCCTGGCCCTAAACTCTGGTACCGGGACTTTCTGCAGTTGGTGGAGCCAGGCGGT GGCGGAGGTGCAAACTCCCTGCGAATGTGCCGCCCGCAGCCCGGGCACCACTCTGTGGCAGCAGATTCAC GTCGTAAGGGTCGCAACAGACGGATGCATGTCTCTGAGCTCCGTGCTGAGCGTGGACCACGTAGTGCAGC TCACTGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291539
Insert Size	2250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291539.1, NP_001278468.1
RefSeq Size	3687 bp
RefSeq ORF	2250 bp
Locus ID	20347
Gene Summary	This gene encodes a secreted protein that belongs to the class 3 semaphorin/collapsin family. Members of this family play a role in growth cone guidance during neurogenesis. The encoded protein inhibits axonal extension. This protein is thought to be an osteoblast protein that regulates bone mass and affects skeletal homeostasis. A similar gene in humans functions as a tumor suppressor gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 3. Variants 1 through 5 encode the same protein.

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