Mfn2 (NM_001285921) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	D630023P19Rik; Fzo
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228872 representing NM_001285921 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCTGCTCTTTTCTCGATGCAACTCCATCGTCACCGTCAAGAAGGATAAGCGACACATGGCTGAAG TGAATGCTTCCCCTCTCAAGCACTTTGTCACTGCCAAGAAAAAGATCAATGGAATCTTTGAGCAGCTGGG GGCCTACATCCAAGAGAGCGCCAGCTTCCTTGAAGACACCCACAGGAACACAGAACTGGACCCGGTTACC ACGGAAGAGCAGGTCCTGGACGTCAAAGGGTACCTGTCCAAGGTCAGGGGTATCAGCGAAGTGCTGGCCA GGCGGCACATGAAGGTGGCTTTTTTTGGCCGGACGAGCAATGGGAAGAGCACCGTGATCAATGCCATGCT CTGGGACAAAGTTCTGCCATCTGGGATTGGTCATACCACCAATTGCTTCCTGCGGGTTGGGGGCACAGAT GGCCATGAGGCCTTCCTCCTCACAGAGGGCTCAGAAGAGAAGAAGAGTGTCAAGACTGTGAACCAACTGG CCCATGCCCTCCATCAGGACGAGCAGTTGCATGCAGGCAGCATGGTGAGTGTGATGTGGCCCAACTCCAA GTGTCCGCTCCTGAAGGATGACCTCGTGCTGATGGACAGCCCTGGGATCGATGTTACCACGGAGCTGGAC AGCTGGATTGATAAGTTTTGCCTGGATGCTGATGTGTTTGTGCTGGTGGCCAACTCAGAGTCCACGCTGA TGCAGACGGAGAAGCAGTTCTTCCACAAAGTGAGTGAACGTCTCTCCCGGCCCAACATCTTCATCCTGAA CAACCGCTGGGATGCGTCTGCCTCGGAGCCTGAGTACATGGAGGAGGTGCGGCGGCAGCACATGGAGCGC TGCACCAGCTTTCTGGTGGATGAGCTGGGCGTGGTGGATCGAGCTCAGGCTGGGGACCGGATCTTCTTCG TGTCTGCCAAGGAGGTTCTCAGCGCCAGGGTCCAGAAAGCCCAGGGCATGCCAGAAGGAGGCGGCGCTCT CGCAGAAGGTTTTCAAGTGAGGATGTTTGAGTTTCAGAATTTCGAGAGGCAGTTTGAGGAGTGCATTTCC CAGTCTGCAGTAAAGACCAAATTTGAGCAGCACACAGTCCGGGCCAAGCAGATTGCAGAGGCCGTTCGTC TCATCATGGATTCCCTGCACATCGCAGCTCAGGAGCAGCGGGTTTATTGCCTAGAAATGCGGGAAGAGCG GCAAGACCGGCTGAGGTTTATTGACAAGCAGCTGGAGCTCCTGGCTCAAGACTACAAGCTGCGAATTAAG CAGATTACGGAGGAAGTGGAAAGGCAGGTGTCCACAGCCATGGCTGAAGAGATCAGGCGCCTCTCTGTGC TAGTTGACGAGTACCAGATGGACTTCCACCCATCCCCAGTTGTCCTCAAGGTTTATAAGAACGAGCTGCA CCGCCATATAGAGGAAGGTCTGGGCCGGAACCTGTCTGACCGCTGCTCCACTGCCATTGCCAGTTCACTG CAGACTATGCAGCAGGACATGATAGACGGCTTGAAGCCCCTTCTTCCTGTATCTATGCGGAATCAGATAG ACATGCTGGTCCCTCGACAGTGTTTCTCCCTCAGCTATGACCTGAATTGTGACAAGCTGTGTGCTGACTT TCAGGAGGACATCGAGTTCCACTTCTCCCTTGGATGGACTATGCTAGTGAACAGGTTCCTGGGCCCCAAG AATAGCCGCCGGGCCTTGCTAGGCTACAGTGATCAGGTTCAGCGTCCTCTCCCTCTGACACCTGCCAACC CCAGCATGCCCCCCTTGCCACAGAGCTCCCTCACCCAGGAGGAGCTCATGGTCTCCATGGTTACTGGCCT GGCCTCTCTGACGTCTAGGACCTCCATGGGCATTCTTGTGGTCGGAGGAGTGGTGTGGAAGGCAGTGGGC TGGAGACTCATCGCCCTCTCCTTTGGACTGTATGGCCTCCTGTACGTCTATGAGCGACTGACCTGGACCA CCAAAGCCAAAGAGAGGGCCTTCAAGCGCCAGTTTGTGGAATACGCCAGTGAGAAGCTACAGCTCATCAT CAGTTACACCGGCTCTAACTGCAGCCACCAAGTCCAGCAGGAATTGTCTGGGACATTTGCTCATCTGTGC CAGCAAGTTGACATCACCCGAGATAATCTGGAGCAGGAAATTGCTGCCATGAACAAGAAAGTCGAGGCTC TGGATTCACTTCAGAGCAGAGCCAAACTGCTCAGGAATAAAGCTGGCTGGTTGGACAGCGAACTCAACAT GTTCACACACCAGTACCTGCAGCCCAGCAGATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285921
Insert Size	2274 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285921.1, NP_001272850.1
RefSeq Size	4542 bp
RefSeq ORF	2274 bp
Locus ID	170731
UniProt ID	Q80U63
Gene Summary	Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:12527753, PubMed:23921378, PubMed:23620051). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks. Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains (By similarity). Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Plays an important role in the regulation of vascular smooth muscle cell proliferation (By similarity). Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PRKN recruitment to dysfunctional mitochondria (PubMed:23620051). Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress (PubMed:23921556). Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions (PubMed:23921556).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. All variants (1 through 7) encode the same protein.

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