Ap1g1 (NM_001301211) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA409002; Adtg; AU041323; AW551707; D8Ertd374e
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229003 representing NM_001301211 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCCCCCATCAGATTGCGGGAGCTGATCCGGACCATCCGGACAGCCCGAACCCAAGCTGAGGAAC GAGAAATGATCCAGAAAGAATGTGCTGCAATCCGGTCCTCATTTAGAGAAGAAGACAATACATACCGGTG TCGGAATGTGGCAAAGTTACTATATATGCACATGCTGGGCTACCCTGCTCACTTTGGACAGTTGGAGTGC CTCAAGCTAATCGCCTCACAAAAATTCACAGACAAACGCATTGGCTATTTAGGAGCAATGCTATTATTAG ATGAAAGACAAGATGTGCATCTTCTCATGACCAACTGTATCAAGAATGATCTTAATCATAGCACGCAGTT TGTGCAGGGGCTAGCACTTTGTACCCTCGGCTGCATGGGCTCCTCGGAGATGTGCAGAGATCTTGCGGGA GAGGTAGAAAAGCTTCTGAAAACCTCCAACTCTTACTTAAGAAAAAAGGCAGCACTGTGTGCTGTTCACG TCATCAGGAAGGTTCCTGAACTTATGGAGATGTTTTTACCAGCAACAAAAAATTTATTGAATGAGAAGAA CCATGGTGTCCTTCACACGTCTGTAGTCCTCCTCACAGAGATGTGTGAACGGAGCCCAGACATGCTCGCA CATTTCAGAAAGCTTGTGCCCCAATTAGTTCGTATTCTAAAGAACCTCATCATGTCCGGATATTCCCCAG AACATGATGTCTCTGGTATCAGTGACCCCTTTTTGCAGGTACGAATTTTGCGGTTATTAAGAATTTTAGG ACGGAATGACGATGATTCAAGTGAAGCTATGAATGATATATTAGCACAGGTTGCCACTAATACAGAGACT AGTAAAAATGTAGGAAATGCTATTCTGTATGAGACGGTTTTGACTATCATGGATATTAAATCAGAGAGTG GATTGCGAGTTCTAGCCATAAATATTCTTGGTCGATTCTTATTGAACAATGACAAAAATATTAGATATGT TGCTCTGACATCTCTGTTGAAGACTGTGCAGACAGATCATAACGCTGTACAGAGGCATAGAAGCACAATT GTGGACTGTCTGAAAGATCTGGATGTCTCCATAAAAAGACGTGCAATGGAACTGAGTTTTGCCCTGGTGA ATGGGAATAATATCCGAGGCATGATGAAAGAATTACTTTATTTTCTGGATTCCTGTGAGCCAGAATTTAA AGCTGATTGTGCATCTGGAATCTTCCTTGCTGCAGAAAAGTACGCACCTTCCAAACGGTGGCATATAGAT ACAATTATGCGTGTCTTGACAACGGCAGGAAGTTATGTTCGCGATGATGCAGTTCCTAACTTGATTCAGT TAATAACCAACAGTGTGGAGATGCACGCTTACACCGTCCAGCGCCTGTACAAGGCGATTCTCGGTGACTA TTCTCAACAACCCTTGGTACAAGTGGCTGCGTGGTGTATAGGTGAATATGGCGATCTTCTTGTGTCTGGC CAGTGTGAAGAGGAAGAGCCTATTCAGGTGACAGAAGATGAAGTGCTGGATATTTTAGAAAGTGTCCTGA TCTCTAATATGTCCACCTCTGTGACAAGAGGCTATGCTCTCACTGCCATTATGAAGCTGTCTACCCGATT CACCTGTACTGTAAACCGAATTAAGAAAGTGGTTTCCATCTATGGGAGCAGCATCGACGTAGAGCTCCAG CAGAGGGCAGTAGAGTACAACGCACTTTTTAAGAAGTATGACCACATGAGGTCTGCCCTACTTGAAAGAA TGCCTGTCATGGAAAAAGTGACCACAAATGGCCCTTCGGAGATCGTGCAGACAAATGGAGAGACAGAACC AGCGCCCCTAGAGACTAAACCACCACCCTCAGGGCCACAACCCACAAGCCAGGCCAATGATCTATTGGAT TTGTTGGGAGGAAATGACATAACACCTGTTATCCCAACTGCACCTACAAGCAAACCAGCATCAGCTGGTG GAGAACTTCTCGACTTGCTAGGAGACATCACCCTGACAGGTGCTCCAGCTGCTGCTCCTACCCCTGCCTC AGTGCCACAGATATCCCAGCCCCCCTTCTTGTTGGATGGGCTTTCCTCACAGCCTCTCTTCAATGACATC GCTCCAGGCATCCCCTCCATCACAGCGTACAGTAAGAACGGCTTGAAGATAGAGTTCACCTTTGAACGGT CAAACACCAACCCCAGCGTAACAGTGATAACGATACAGGCCTCTAACAGCACAGAGCTAGACATGACGGA CTTTGTTTTCCAGGCTGCAGTACCAAAGACATTCCAGCTGCAGCTTCTGTCTCCTAGCAGCAGCGTTGTC CCAGCCTTTAATACAGGGACCATCACACAAGTCATTAAAGTTCTGAATCCACAGAAGCAACAGCTGCGAA TGCGGATCAAGCTCACATATAATCACAAGGGCTCGGCAATGCAAGATCTAGCAGAAGTGAACAATTTCCC CCCTCAGTCCTGGCAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301211
Insert Size	2469 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301211.1, NP_001288140.1
RefSeq Size	6882 bp
RefSeq ORF	2469 bp
Locus ID	11765
UniProt ID	P22892
Gene Summary	Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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