Ano7 (NM_001271884) Mouse Untagged Clone

CAT#: MC229033

Ano7 (untagged) - Mouse anoctamin 7 (Ano7), transcript variant 2



  "NM_001271884" in other vectors (1)

CNY 8,080.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
ANO7 Antibody - middle region
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms IPC; NG; NGE; Ngep; Ngep-L; Pc; Pcanap5; Tmem16g
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229033 representing NM_001271884
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCGGGGGCAAGCGCGAGAAGAAGACAGCGTGGTGCTGATCGACATGGCCTCCCCTGAAGCAGGGA
ATGGATGCTCCTATGGGAGCACAGCCCAAGCCTCAGAGGCAGGTAAACAGCAGGTGGCCCCCAGCAGAGT
TGGGAGCTCTGCCAAACCCCCAATTGATTTTGTTCTTGTTTGGGAAGAAGACCTGAGGAACCAAGAGAAC
CCCACGAAGGACAAGACAGACACACACGAGGTCTGGCGGGAGACTTTTCTGGAGAATCTTTGCTTGGCTG
GCCTGAAAATAGATCAGCATGATGTCCAGGATGAGGCCGCTGCAGTTCATTACATCCTCCTCCGCGCACC
CTGGGCTGTGCTTTGCTACTATGCAGAAGACCTGCGCCTGAAGCTGCCTCTACAGGAGTTACCCAACCAG
GCCTCCAACTGGTCAGCCACCCTTCTGGAGTGGCTGGGCATCCCCAATATCCTGCTGGAGCACGTGCCTG
ACACGCCCCCCGAGTATTACTCCTGCCAGTTCAAAGCAAGCAAGCTGCAATGGTTTCTTGGGAGTGACAA
CCAAGACACCTTCTTCACCAGCACCAAGAGGCATCAGATTCTATTTGAGATCCTGGCCAAGACTCCATAT
GGGCACGAGAAGAAAGGCCTGTTTGGGATTGACCAGCTGCTGGCAGAAGGTGTATTCAGTGCAGCCTTCC
CTCTGCATGACGGCCCATTCTCTGCAGTCCCAGAGAGCTCACAGGTCCTGGGTCTCATCCAACGCCAAGT
CTTATTCCAGCACTGGGCTCGCTGGGGCAAGTGGAACAAGTACCAGCCATTGGACCATGTGCGAAGGTAC
TTCGGGGAGAAGGTGGCTCTCTACTTCGCCTGGCTTGGGTTTTACACTGGTTGGCTCCTGCCTGCGGCAG
TAGTGGGCACAGTGGTCTTCCTGGTGGGATGTTTCCTGGTGTTCTCAGACATACCAACGCAGGAGCTGTG
TCACAGCTCAGACAGCTTCGACATGTGCCCGCTGTGCTCCGACTGTTCTTTTTGGCTGCTCTCCAGTGCC
TGCACCCTGGCCCAGGCTGGGCGGCTCTTCGACCATGGCGGCACCGTCTTCTTCAGTTTGTTCATGGCAC
TGTGGGCGGTGCTGCTTCTGGAGTACTGGAAGCGGAAAAACGCCACATTGGCCTACCGCTGGGACTGTTC
TGACTATGAAGACATTGAGGAGAGGCCTCGGCCCCAGTTCGCTGCAACGGCCCCCATGACAGCCCTGAAC
CCCATCACGGGGGAAGATGAGCCCTACTTTCCTGAGAAGAATCGTGTACGCCGCATGCTGGCTGGTTCTG
TGGTGCTCTTGATGATGGTGGCTGTGGTGATTATGTGCCTTGTATCTGTCATCCTGTACCGGGCCGTCAT
GGCTATCATTGTGTCCAGGTCAGACAACGCCTTCCTCTCTGCCTGGGCTTCACGGATTGCCAGCCTCACA
GGGTCAGTGGTGAACCTTGTCTTCATCCTCATCCTCTCCAAGGTCTATGTGCTCCTGGCCCAGGTCCTAA
CAAGATGGGAGATGCACCGGACACAGACCGAGTTTGAGGATGCCTTCACCCTCAAGGTGTTCATCTTCCA
GTTTGTGAATTTCTACGCCTCGCCCGTGTATATCGCCTTCTTCAAGGGCAGGTTTGTGGGATACCCGGGC
AACTACCACACCTTGTTTGGAATCCGAAATGAGGAGTGTCCAGCTGGAGGCTGCCTCAGTGAGCTGGCAC
AGGAGCTCCTGGTCATCATGGTGGGCAAGCAGATCATCAATAACGTGCAGGAGGTTCTTGTCCCAAAGCT
GAAAGGCTGCTGGCAGAAGTTCTCCAGGGGCAAGAAGGCTGGCACGGGGACCCACCCAGCACCTTGGGAG
GCCGACTATGAGTTGCTGCCTTGTGAGGGGCTGTTTCACGAATACCTTGAAATGGTGCTGCAGTTTGGAT
TCGTCACCATCTTCGTAGCTGCCTGCCCGCTGGCGCCGCTTTTTGCCCTACTCAACAACTGGGTGGAGAT
CCGCCTAGATGCGCGCAAGTTCGTGTGCGAGTATCGGCGCCCAGTGGCGGAGCGCGCCCAGGACATCGGC
ATCTGGTTCCACATCCTAACGGGCCTCACACACCTCGCGGTCATCAGCAATGCCTTCCTGCTGGCCTTCT
CCTCCGACTTCCTGCCACGTGTCTACTACAGCTGGACGCACGCCCCTGACCTGCATGGCTTCCTCAATTT
CACGCTGGCACGAGCACCGCCCACTTTCACCTCCGCACACAACCGCACTTGCAGGTACCGGGCTTTCCGG
GATGATGATGGACACTATTCTCCGACTTACTGGACTCTCCTGGCCATCCGCCTGGCCTTTGTCATCGTAT
TTGAGCATGTGGTGTTCTCCATTGGCCGTGTTCTGGATCTCTTGGTTCCTGACATCCCAGAATCCGTGGA
GATCAAGGTGAAGCGGGAATACTATTTAGCTAAACAAGCACTGGCTGAGAACGAGGTCACAGTAGGGGTT
ACCGGAGGCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001271884
Insert Size 2532 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001271884.1, NP_001258813.1
RefSeq Size 3899 bp
RefSeq ORF 2532 bp
Locus ID 404545
UniProt ID Q14AT5
Gene Summary This gene encodes a member of the anoctamin family, which in mammals is comprised of 10 members. Anoctamin proteins are proposed to have eight transmembrane domains with both termini facing the cytoplasm and a C-terminal domain of unknown function. While some members have been characterized as calcium-activated chloride channels, this protein is reported to have little anion conductance activity. In humans, this protein is primarily found in prostate tissues and may serve as a target for prostate cancer immunotherapy. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Dec 2012]
Transcript Variant: This variant (2) uses an alternate acceptor splice site in the 3' coding region which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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