Ano7 (NM_001271884) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	IPC; NG; NGE; Ngep; Ngep-L; Pc; Pcanap5; Tmem16g
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229033 representing NM_001271884 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCGGGGGCAAGCGCGAGAAGAAGACAGCGTGGTGCTGATCGACATGGCCTCCCCTGAAGCAGGGA ATGGATGCTCCTATGGGAGCACAGCCCAAGCCTCAGAGGCAGGTAAACAGCAGGTGGCCCCCAGCAGAGT TGGGAGCTCTGCCAAACCCCCAATTGATTTTGTTCTTGTTTGGGAAGAAGACCTGAGGAACCAAGAGAAC CCCACGAAGGACAAGACAGACACACACGAGGTCTGGCGGGAGACTTTTCTGGAGAATCTTTGCTTGGCTG GCCTGAAAATAGATCAGCATGATGTCCAGGATGAGGCCGCTGCAGTTCATTACATCCTCCTCCGCGCACC CTGGGCTGTGCTTTGCTACTATGCAGAAGACCTGCGCCTGAAGCTGCCTCTACAGGAGTTACCCAACCAG GCCTCCAACTGGTCAGCCACCCTTCTGGAGTGGCTGGGCATCCCCAATATCCTGCTGGAGCACGTGCCTG ACACGCCCCCCGAGTATTACTCCTGCCAGTTCAAAGCAAGCAAGCTGCAATGGTTTCTTGGGAGTGACAA CCAAGACACCTTCTTCACCAGCACCAAGAGGCATCAGATTCTATTTGAGATCCTGGCCAAGACTCCATAT GGGCACGAGAAGAAAGGCCTGTTTGGGATTGACCAGCTGCTGGCAGAAGGTGTATTCAGTGCAGCCTTCC CTCTGCATGACGGCCCATTCTCTGCAGTCCCAGAGAGCTCACAGGTCCTGGGTCTCATCCAACGCCAAGT CTTATTCCAGCACTGGGCTCGCTGGGGCAAGTGGAACAAGTACCAGCCATTGGACCATGTGCGAAGGTAC TTCGGGGAGAAGGTGGCTCTCTACTTCGCCTGGCTTGGGTTTTACACTGGTTGGCTCCTGCCTGCGGCAG TAGTGGGCACAGTGGTCTTCCTGGTGGGATGTTTCCTGGTGTTCTCAGACATACCAACGCAGGAGCTGTG TCACAGCTCAGACAGCTTCGACATGTGCCCGCTGTGCTCCGACTGTTCTTTTTGGCTGCTCTCCAGTGCC TGCACCCTGGCCCAGGCTGGGCGGCTCTTCGACCATGGCGGCACCGTCTTCTTCAGTTTGTTCATGGCAC TGTGGGCGGTGCTGCTTCTGGAGTACTGGAAGCGGAAAAACGCCACATTGGCCTACCGCTGGGACTGTTC TGACTATGAAGACATTGAGGAGAGGCCTCGGCCCCAGTTCGCTGCAACGGCCCCCATGACAGCCCTGAAC CCCATCACGGGGGAAGATGAGCCCTACTTTCCTGAGAAGAATCGTGTACGCCGCATGCTGGCTGGTTCTG TGGTGCTCTTGATGATGGTGGCTGTGGTGATTATGTGCCTTGTATCTGTCATCCTGTACCGGGCCGTCAT GGCTATCATTGTGTCCAGGTCAGACAACGCCTTCCTCTCTGCCTGGGCTTCACGGATTGCCAGCCTCACA GGGTCAGTGGTGAACCTTGTCTTCATCCTCATCCTCTCCAAGGTCTATGTGCTCCTGGCCCAGGTCCTAA CAAGATGGGAGATGCACCGGACACAGACCGAGTTTGAGGATGCCTTCACCCTCAAGGTGTTCATCTTCCA GTTTGTGAATTTCTACGCCTCGCCCGTGTATATCGCCTTCTTCAAGGGCAGGTTTGTGGGATACCCGGGC AACTACCACACCTTGTTTGGAATCCGAAATGAGGAGTGTCCAGCTGGAGGCTGCCTCAGTGAGCTGGCAC AGGAGCTCCTGGTCATCATGGTGGGCAAGCAGATCATCAATAACGTGCAGGAGGTTCTTGTCCCAAAGCT GAAAGGCTGCTGGCAGAAGTTCTCCAGGGGCAAGAAGGCTGGCACGGGGACCCACCCAGCACCTTGGGAG GCCGACTATGAGTTGCTGCCTTGTGAGGGGCTGTTTCACGAATACCTTGAAATGGTGCTGCAGTTTGGAT TCGTCACCATCTTCGTAGCTGCCTGCCCGCTGGCGCCGCTTTTTGCCCTACTCAACAACTGGGTGGAGAT CCGCCTAGATGCGCGCAAGTTCGTGTGCGAGTATCGGCGCCCAGTGGCGGAGCGCGCCCAGGACATCGGC ATCTGGTTCCACATCCTAACGGGCCTCACACACCTCGCGGTCATCAGCAATGCCTTCCTGCTGGCCTTCT CCTCCGACTTCCTGCCACGTGTCTACTACAGCTGGACGCACGCCCCTGACCTGCATGGCTTCCTCAATTT CACGCTGGCACGAGCACCGCCCACTTTCACCTCCGCACACAACCGCACTTGCAGGTACCGGGCTTTCCGG GATGATGATGGACACTATTCTCCGACTTACTGGACTCTCCTGGCCATCCGCCTGGCCTTTGTCATCGTAT TTGAGCATGTGGTGTTCTCCATTGGCCGTGTTCTGGATCTCTTGGTTCCTGACATCCCAGAATCCGTGGA GATCAAGGTGAAGCGGGAATACTATTTAGCTAAACAAGCACTGGCTGAGAACGAGGTCACAGTAGGGGTT ACCGGAGGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271884
Insert Size	2532 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271884.1, NP_001258813.1
RefSeq Size	3899 bp
RefSeq ORF	2532 bp
Locus ID	404545
UniProt ID	Q14AT5
Gene Summary	This gene encodes a member of the anoctamin family, which in mammals is comprised of 10 members. Anoctamin proteins are proposed to have eight transmembrane domains with both termini facing the cytoplasm and a C-terminal domain of unknown function. While some members have been characterized as calcium-activated chloride channels, this protein is reported to have little anion conductance activity. In humans, this protein is primarily found in prostate tissues and may serve as a target for prostate cancer immunotherapy. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Dec 2012] Transcript Variant: This variant (2) uses an alternate acceptor splice site in the 3' coding region which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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