Dnm1 (NM_001301737) Mouse Untagged Clone

CAT#: MC229045

Dnm1 (untagged) - Mouse dynamin 1 (Dnm1), transcript variant 2



  "NM_001301737" in other vectors (1)

CNY 8,270.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI838169; Dnm; Ftf; Ftfl; mKIAA4093
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229045 representing NM_001301737
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAACCGCGGCATGGAAGACCTCATCCCGCTGGTTAACCGGTTACAGGACGCCTTCTCCGCCATCG
GCCAGAACGCGGACCTCGACCTGCCGCAGATCGCCGTGGTAGGCGGCCAGAGCGCCGGCAAGAGCTCGGT
GCTGGAGAATTTCGTGGGCAGGGACTTCTTGCCCCGAGGATCTGGCATCGTCACCCGGCGTCCCCTGGTC
CTGCAGCTGGTTAATTCTACCACAGAATATGCCGAGTTCCTGCACTGCAAGGGGAAGAAATTCACCGACT
TCGAGGAGGTGCGCCTGGAGATCGAGGCTGAGACCGATCGAGTCACCGGCACCAACAAGGGCATTTCGCC
AGTGCCCATCAACCTGCGGGTCTACTCGCCCCATGTGCTGAACCTGACTCTAGTGGACCTGCCAGGAATG
ACCAAGGTCCCAGTTGGGGACCAACCTCCTGATATCGAGTTCCAGATCCGGGACATGCTTATGCAGTTCG
TCACTAAGGAGAACTGCCTTATCCTGGCTGTGTCCCCTGCCAACTCGGATTTGGCCAACTCTGATGCCCT
CAAGATCGCTAAGGAGGTGGACCCCCAGGGTCAGCGCACCATTGGGGTCATCACCAAGTTGGACCTGATG
GACGAGGGCACAGATGCGCGGGACGTGCTAGAGAACAAGCTGCTCCCTTTGCGCAGAGGTTACATCGGCG
TGGTGAACCGGAGCCAGAAGGACATAGACGGCAAGAAGGACATCACAGCCGCCTTGGCTGCAGAGCGCAA
ATTCTTCCTCTCTCACCCATCCTACCGCCACTTGGCTGACCGCATGGGCACACCCTACCTGCAGAAGGTC
CTCAATCAGCAATTGACCAACCACATCCGGGACACACTGCCGGGACTTCGGAACAAGCTGCAGAGCCAGC
TGCTGTCCATTGAGAAGGAGGTGGACGAGTACAAGAACTTCCGACCGGATGACCCAGCGCGCAAGACCAA
GGCCCTGCTGCAGATGGTCCAGCAGTTTGCAGTGGACTTCGAGAAGCGCATCGAGGGTTCTGGAGACCAG
ATTGACACTTACGAGCTGTCAGGTGGAGCCCGCATTAACCGGATCTTCCATGAACGCTTCCCCTTTGAGC
TGGTTAAGATGGAGTTTGATGAGAAGGAACTGCGAAGGGAGATCAGCTATGCTATCAAAAATATCCATGG
CATCAGGACGGGCCTCTTCACACCTGACCTCGCTTTTGAAGCCACAGTGAAAAAGCAGGTGCAGAAGCTC
AAAGAGCCCAGTATCAAGTGTGTGGACATGGTAGTCAGTGAACTCACGTCCACCATCAGAAAGTGTAGTG
AAAAGCTGCAGCAATACCCGCGTCTGCGGGAGGAGATGGAGCGAATTGTGACCACCCACATCCGGGAACG
TGAGGGCCGCACCAAGGAGCAGGTCATGCTTCTCATCGACATTGAGCTGGCTTACATGAATACCAACCAC
GAAGACTTCATAGGCTTTGCCAATGCTCAGCAGAGAAGCAACCAGATGAACAAGAAGAAGACTTCAGGGA
ACCAGGATGAGATTCTGGTCATTCGAAAGGGGTGGTTGACCATCAACAACATCGGCATCATGAAGGGAGG
CTCCAAGGAGTACTGGTTTGTGCTGACTGCTGAGAATCTGTCCTGGTACAAGGATGATGAGGAGAAAGAG
AAGAAGTACATGCTGTCTGTGGACAATCTGAAGCTGCGTGATGTGGAGAAGGGCTTCATGTCAAGCAAGC
ATATTTTTGCCCTCTTCAACACAGAGCAGAGGAATGTCTACAAGGATTACCGGCAGCTGGAACTGGCCTG
TGAGACACAGGAGGAGGTGGACAGTTGGAAGGCTTCCTTCCTGAGGGCTGGCGTGTACCCTGAGCGTGTT
GGGGACAAAGAGAAAGCCAGTGAGACCGAGGAGAACGGCTCTGACAGCTTCATGCACTCGATGGACCCTC
AGCTGGAGCGCCAGGTGGAGACCATCCGGAACCTGGTAGACTCGTACATGGCCATTGTCAACAAGACTGT
GCGGGACCTCATGCCCAAGACCATCATGCACCTCATGATCAACAACACCAAGGAGTTTATCTTCTCTGAG
CTGCTGGCCAACCTGTACTCTTGCGGGGACCAGAACACACTGATGGAAGAATCGGCCGAGCAGGCTCAGC
GGCGCGACGAGATGCTGCGCATGTACCACGCACTGAAGGAGGCGCTCAGTATTATCGGCGACATCAACAC
GACCACCGTCAGCACGCCCATGCCCCCGCCCGTGGACGACTCCTGGCTGCAGGTGCAGAGCGTACCGGCC
GGACGCAGATCGCCCACGTCCAGCCCCACGCCGCAGCGCCGAGCCCCCGCCGTGCCCCCAGCCCGGCCCG
GATCGCGGGGCCCTGCTCCTGGGCCTCCGCCTGCTGGATCCGCCCTGGGGGGGGCGCCCCCCGTGCCCTC
CAGGCCGGGGGCTTCCCCTGACCCCTTTGGCCCCCCTCCCCAGGTGCCCTCGCGCCCCAACCGCGCCCCG
CCTGGGGTCCCCAGAATCACTATCAGTGACCCCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001301737
Insert Size 2556 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001301737.1, NP_001288666.1
RefSeq Size 3295 bp
RefSeq ORF 2556 bp
Locus ID 13429
UniProt ID P39053
Gene Summary This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein is a GTPase which is required for membrane recycling, including vesicle endocytosis in neurons. It may also be involved in cellular fission via association with microtubules and actin filaments. Mutations in this gene have been shown to cause seizures. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]
Transcript Variant: This variant (2) has multiple differences in the coding region, one of which results in a frameshift, compared to variant 1. The resulting protein (isoform 2) has a distinct C-terminus and is shorter than isoform 1. This variant contains the alternate 'a' exon in the central coding region and the 'b' splicing pattern in the 3' coding region (PMID:10398046, PMID:20700442).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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