Amot (NM_001290274) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CAG-2; D0Kist1; Sii6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229049 representing NM_001290274 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAAGTTCTGACGATCAGCCAAGTGGAGGAACGACGGTACTGCAACGGCTGCTTCAAGAGCAGCTTC GCTATGGCAATCCCAGTGAGAATCGCAGTCTGCTTGCCATACACCAGCAAGCCACAGGGAATAGCTCTCC ATTTTCCACGGGCAGTGGGAACCAAGGCCCTCAGAATGATGTATTGAGTTCCCAGGATCACCACCAGCAG CAGCTTGTGGCACACCCTGCTCGCCAAGAGCCCCAGGGTCAGGAAATCCAGTCAGAAAACGGCGTCATGG AGAAGCAGCTGTCCCCTCGAATGCAGAATAACGAAGAACTCCCAACCTATGAAGAGGCCAAGGTCCAGTC CCAGTACTTCAGGGGCCAGCAGCATGCTAGTGTTGGGGCTGCTTTCTATGTCACAGGAGTCACCAACCAG AAGATGAGAACCGAGGGACGCCCGTCAGTCCAGCGCCTCACGCCCGGGAAGATGCACCAAGATGAAGGCC TTCGAGATCTTAAGCAAGGGCATGTCCGATCCTTGAGTGAAAGACTGATGCAGATGTCATTGGCTACCAG TGGAGTTAAAGCCCACCCACCTGTTACCAGTGCTCCTCTCTCCCCACCACAACCCAATGATCTCTACAAG AATGCCACCAGTTCCAGTGAATTCTACAAGGCCCAAGGGCCACCTCCAAGCCAACATAGTCTGAAGGGCA TGGAACACCGAGGTCCCCCGCCAGAATACCCTTTCAAGGGCGTGCCATCCCAGTCTGTAGTGTGCAAGTC CCAAGAGCCAGGGCACTTCTATAGTGAGCATCGCCTGAACCAGCCAGGGAGAACAGAGGGGCAACTGATG AGGTATCAGCACCCCCCTGAGTATGGGGCAGCCAGGGCAACACAGGACATCTCCTCGCTGTCTTTGTCAG CCAGGAACTCTCAACCTCATAGCCCTACTTCTTCCCTGACAGCTGGGGCTTCCAGCTTGCCCTTGCTGCA GTCTCCGCCGTCCACTAGATTGCCTCCTGGTCAACACCTGGTCTCGAACCAAGGTGACCACTCTGCTCAC CTGTCAAGGCATCAGCAGCATCTTCTTTCGAGTCAGAGTCATCAGGGGGACCACTACCGTCACGCCCAGG CCAGCCTGACTTCAGCGCAGCAGCAGCCAGGAGAAGCCTACTCAGCTATGCCTCGGGCTCAGCAGTCTGC TTCTTATCAGCCCATGCCAGCTGACCCTTTTGCTATGGTCTCTAGAGCTCAGCAGATGGTTGAGATCCTG TCTGATGAGAATCGCAACTTGCGGCAGGAGTTGGATGGATGCTATGAGAAGGTGGCGAGACTGCAGAAGG TGGAGACAGAAATCCAACGGGTCTCAGAGGCTTATGAGAACTTGGTGAAGTCATCTTCCAAAAGAGAGGC TCTGGAGAAAGCCATGAGGAACAAGCTGGAGGGCGAGATTAGAAGGATGCATGACTTCAACAGAGATCTG AGAGACCGTCTAGAGACTGCCAACAAGCAGCTGGCAGAGAAGGAGTACGAGGGGTCCGAGGACACCAGGA AGACCATCTCGCAGCTCTTTGCCAAACATAAAGAAAACCAGCGGGAGAAGGAGAAGCTGGAAGCTGAGCT GGCTACTGCCCGTTCTACCAATGAAGACCAAAGGCGACACATTGAAATCCGAGACCAGGCATTGAGCAAT GCCCAGGCGAAGGTGGTGAAGCTGGAAGAAGAGCTGAAAAAGAAGCAAGTGTACGTAGATAAGGTGGAGA AGATGCAGCAGGCCCTTGTGCAGCTCCAGGCCGCCTGTGAGAAGCGTGAGCAGCTGGAGCACCGGCTCCG GACACGTTTGGAGAGGGAACTGGAATCCCTTAGAATCCAGCAGCGCCAGGGCAACTCCCAGCCCACTAAT GCTTCAGAATACAATGCCGCTGCACTGATGGAGCTCCTCCGGGAGAAGGAAGAGAGAATTCTGGCCCTGG AAGCAGATATGACCAAGTGGGAGCAAAAGTATTTGGAAGAGAATGTGATGAGACACTTTGCTCTGGATGC TGCTGCAACTGTGGCTGCACAGAGAGACACAACAGTCATTAGCCACTCTCCTAACACCAGCTATGACACA GCCTTAGAGGCCCGCATCCAGAAGGAAGAGGAAGAAATCCTGATGGCCAACAAGCGTTGCTTGGACATGG AAGGAAGGATTAAGACCCTCCATGCCCAGATTATTGAAAAGGATGCCATGATCAAGGTGCTGCAGCAGCG CTCCAGAAAGGAGCCGAGTAAAACAGAGCAACTCTCATCCATGCGGCCAGCAAAGTCTCTGATGTCCATT TCCAATGCTGGATCAGGCCTCCTTGCACACTCCTCTACCTTGACTGGTGCGCCCATTATGGAGGAGAAGC GTGATGACAAGAGCTGGAAGGGGAGCCTAGGTAAGGAAAAGGAAAGCAACAGAAGCAAAGGCACAGTGAC AGACTTAGAAAGTGTCCTGACTCTGTTACACACAGCAAGGAAAAGGGACAATGGTCCAGGTTCTAGAGAG GAGAATTTGGAGTCCCCCCTCTCAATGGAATTAGATTTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290274
Insert Size	2562 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290274.1, NP_001277203.1
RefSeq Size	2937 bp
RefSeq ORF	2562 bp
Locus ID	27494
UniProt ID	Q8VHG2
Gene Summary	Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks several exons and it's 3'-terminal exon extends past a splice site that is used in variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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