Sema4d (NM_001281880) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CD100; coll-4; Semacl2; Semaj; Semcl2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229062 representing NM_001281880 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGATGTGTGCCCCCGTTAGGGGGCTGTTCTTGGCCCTGGTGGTAGTGTTGAGAACCGCGGTGGCAT TTGCACCTGTGCCTCGGCTCACCTGGGAACATGGAGAGGTAGGTCTGGTGCAGTTTCACAAGCCAGGCAT CTTTAACTACTCGGCCTTGCTGATGAGTGAGGACAAAGACACTCTGTATGTAGGCGCCCGGGAAGCAGTC TTTGCAGTGAATGCGCTGAACATCTCTGAGAAGCAACATGAGGTATATTGGAAGGTCTCTGAAGACAAAA AATCCAAGTGTGCAGAGAAGGGGAAATCAAAGCAGACGGAATGCCTAAACTACATTCGAGTACTACAGCC ACTAAGCAGCACTTCCCTCTATGTGTGTGGGACCAATGCGTTCCAGCCCACCTGTGACCACCTGAACTTG ACATCCTTCAAGTTTCTGGGGAAAAGTGAAGATGGCAAAGGAAGATGCCCCTTCGACCCCGCCCACAGCT ACACATCAGTCATGGTTGGGGGCGAGCTCTACTCTGGGACGTCCTATAATTTCTTGGGCAGTGAACCCAT CATCTCTCGAAACTCTTCCCACAGTCCCTTGAGGACGGAGTATGCCATCCCGTGGCTGAACGAGCCTAGC TTCGTCTTTGCTGACGTGATCCAGAAAAGCCCAGATGGTCCGGAGGGTGAAGATGACAAGGTCTACTTCT TTTTTACGGAGGTATCCGTGGAGTACGAATTCGTCTTCAAGTTGATGATCCCGCGAGTTGCCAGGGTGTG CAAGGGCGACCAGGGCGGCCTGCGGACTTTGCAAAAAAAGTGGACCTCCTTCCTAAAGGCCAGGCTGATC TGCTCCAAGCCAGACAGTGGCCTGGTCTTCAACATACTTCAGGATGTGTTTGTGCTGAGGGCCCCGGGCC TCAAGGAGCCTGTGTTCTATGCGGTCTTCACCCCACAGCTGAACAATGTGGGTCTGTCAGCGGTGTGCGC CTACACACTGGCCACGGTGGAGGCAGTCTTCTCCCGTGGAAAGTACATGCAGAGTGCCACAGTGGAGCAG TCTCACACCAAGTGGGTGCGCTACAATGGCCCAGTGCCCACTCCCCGACCTGGAGCGTGTATCGACAGTG AGGCCCGGGCAGCCAACTACACCAGCTCCTTGAATCTCCCAGACAAAACACTGCAGTTTGTAAAAGACCA CCCTTTGATGGATGACTCAGTGACCCCGATAGACAACAGACCCAAGCTGATCAAAAAAGATGTAAACTAC ACCCAGATAGTGGTAGACAGGACCCAGGCCCTGGATGGGACTTTCTACGACGTCATGTTCATCAGCACAG ACCGGGGAGCTCTGCATAAAGCAGTCATCCTCACAAAAGAGGTGCATGTCATCGAGGAGACCCAACTCTT CCGGGACTCTGAACCGGTCCTAACTCTGCTGCTATCGTCAAAGAAGGGGAGGAAGTTTGTCTATGCAGGC TCCAACTCTGGAGTGGTCCAAGCGCCCCTGGCATTCTGCGAAAAGCACGGTAGCTGTGAAGACTGTGTGT TAGCACGGGACCCCTACTGTGCCTGGAGCCCAGCCATCAAGGCCTGTGTTACCCTGCACCAGGAAGAGGC CTCCAGCAGGGGCTGGATTCAGGACATGAGCGGTGACACATCCTCATGCCTGGATAAGAGTAAAGAAAGT TTCAACCAGCATTTTTTCAAGCACGGCGGCACAGCGGAACTCAAATGTTTCCAAAAGTCCAACCTAGCCC GGGTGGTATGGAAGTTCCAGAATGGCGAGTTGAAGGCCGCAAGTCCCAAGTACGGCTTTGTGGGCAGGAA GCACCTGCTCATCTTCAACCTGTCGGACGGAGACAGCGGCGTGTACCAGTGCCTGTCAGAGGAAAGGGTG AGGAATAAAACGGTCTCCCAGCTGCTGGCCAAGCACGTTCTGGAAGTGAAGATGGTACCTCGGACCCCCC CCTCACCTACCTCAGAGGATGCTCAGACAGAAGGTAGTAAGATCACATCCAAAATGCCGGTTGCATCTAC CCAGGGGTCCTCTCCCCCTACCCCGGCTCTGTGGGCAACCTCCCCCAGAGCCGCCACCCTACCTCCCAAG TCCTCCTCCGGCACATCCTGTGAACCAAAGATGGTCATCAACACGGTCCCCCAGCTCCACTCAGAGAAGA CGGTGTATCTCAAGTCCAGTGACAACCGCCTGCTCATGTCTCTCCTCCTCTTCATCTTTGTCCTCTTCCT CTGCCTCTTTTCCTACAACTGCTACAAGGGCTACCTGCCCGGACAGTGCTTAAAATTCCGCTCAGCCCTG CTGCTTGGAAAGAAAACACCCAAGTCAGACTTCTCTGACCTGGAGCAGAGTGTGAAGGAGACACTGGTCG AGCCTGGGAGCTTCTCCCAGCAGAACGGCGACCACCCCAAGCCAGCCCTGGATACGGGCTATGAAACGGA GCAGGACACCATCACCAGCAAAGTCCCCACGGATCGTGAGGACTCGCAACGGATCGATGAACTCTCTGCC CGGGACAAACCGTTTGATGTCAAGTGTGAACTGAAGTTTGCAGATTCGGATGCTGACGGGGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001281880
Insert Size	2586 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281880.1, NP_001268809.1
RefSeq Size	4555 bp
RefSeq ORF	2586 bp
Locus ID	20354
UniProt ID	O09126
Gene Summary	Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (By similarity). Regulates GABAergic synapse development (PubMed:23699507, PubMed:29981480). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (PubMed:23699507, PubMed:29981480). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (By similarity). Promotes the migration of cerebellar granule cells (PubMed:17554007). Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (By similarity). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.

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