Itch (NM_001243712) Mouse Untagged Clone

CAT#: MC229067

Itch (untagged) - Mouse itchy, E3 ubiquitin protein ligase (Itch), transcript variant 2



  "NM_001243712" in other vectors (1)

CNY 8,360.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 6720481N21Rik; 8030492O04Rik; A130065M08; AIP4; C230047C07Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC229067 representing NM_001243712
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGACAGTGGACCACAGCTTGATTCCATGGGTAGTCTGACCATGAAATCTCAACTTCAGATCACTG
TCATCTCAGCCAAACTTAAAGAAAATAAAAAGAATTGGTTTGGACCAAGTCCTTATGTAGAAGTCACAGT
AGATGGACAGTCAAAGAAGACAGAAAAATGCAATAATACAAACAGTCCCAAGTGGAAGCAGCCCCTCACA
GTTATTGTTACCCCTACGAGTAAATTATGTTTTCGTGTGTGGAGTCACCAGACCCTGAAGTCTGATGTTT
TATTGGGAACTGCTGGATTAGATATTTATGAAACATTAAAGTCAAACAATATGAAACTTGAAGAAGTAGT
TATGACTTTGCAGCTTGTAGGTGACAAAGAGCCAACAGAGACGATGGGAGATTTGTCAGTTTGTCTTGAT
GGGCTGCAAGTAGAAGCTGAGGTTGTTACTAACGGTGAAACGTCATGCTCCGAGAGTACTACACAGAATG
ATGATGGCTGCAGAACCAGAGATGATACAAGAGTGAGCACAAATGGATCAGAGGACCCTGAGGTTGCAGC
GTCAGGGGAAAACAAGAGGGCCAATGGGAACAATTCTCCGTCCCTTTCAAATGGTGGTTTTAAGCCTTCT
AGACCTCCTAGACCTTCGAGACCACCTCCACCCACCCCACGAAGACCAGCTTCTGTCAATGGCTCACCAT
CCACGAATTCTGACAGTGATGGATCTAGTACAGGCTCCTTGCCACCAACAAATACAAATGTAAATACAAG
TACATCTGAAGGAGCAACATCTGGATTAATAATTCCTCTTACTATATCTGGAGGCTCGGGCCCTAGGCCT
CTGAATACTGTAAGCCAAGCTCCCCTACCACCTGGGTGGGAGCAGAGAGTAGACCAGCATGGGCGTGTTT
ACTATGTAGACCATGTTGAAAAGCGAACAACATGGGATAGACCAGAACCTCTACCTCCTGGCTGGGAACG
GCGTGTGGACAATATGGGACGTATTTATTATGTTGATCATTTCACAAGAACCACAACATGGCAGAGGCCA
ACCTTGGAATCTGTCCGGAACTATGAACAGTGGCAGCTACAGCGTAGTCAGCTTCAGGGAGCAATGCAGC
AGTTTAACCAGAGATTCATCTATGGGAATCAAGATTTGTTTGCTACATCACAAAACAAAGAATTTGATCC
GCTTGGTCCGTTGCCCCCTGGATGGGAGAAGAGAACTGATAGCAACGGCAGAGTGTATTTTGTCAACCAC
AACACTCGGATTACTCAGTGGGAAGACCCCAGAAGCCAAGGTCAGTTAAATGAAAAGCCCTTACCAGAAG
GCTGGGAAATGAGATTCACAGTGGATGGAATTCCATATTTTGTGGACCACAATAGAAGAGCAACTACTTA
TATAGATCCACGAACGGGAAAATCAGCCTTAGACAATGGGCCCCAGATAGCCTATGTGCGGGACTTCAAG
GCAAAAGTTCAGTATTTCCGGTTCTGGTGCCAGCAACTGGCCATGCCACAGCATATAAAGATCACAGTGA
CAAGAAAAACATTGTTTGAGGATTCCTTTCAGCAGATCATGAGCTTCAGCCCGCAAGACCTGAGAAGACG
TTTGTGGGTGATTTTCCCAGGAGAAGAAGGTTTAGATTATGGAGGTGTAGCAAGAGAATGGTTCTTTCTT
TTGTCACATGAAGTGTTGAACCCAATGTATTGCCTGTTTGAATATGCAGGGAAGGATAACTACTGCCTGC
AGATAAACCCCGCTTCTTACATCAATCCAGACCACCTGAAATACTTTCGTTTTATTGGCAGATTTATTGC
CATGGCTCTGTTTCATGGGAAATTCATAGATACCGGATTTTCTTTACCATTCTATAAACGTATCCTGAAT
AAACCAGTTGGACTGAAGGATTTAGAATCTATTGATCCAGAATTTTATAATTCGCTCATCTGGGTTAAAG
AAAACAACATTGAAGAATGTGGTTTGGAAATGTACTTCTCAGTTGATAAAGAAATTCTAGGTGAAATTAA
GAGTCATGATTTGAAACCCAATGGTGGCAATATTCTTGTGACAGAAGAAAACAAGGAGGAATACATCAGG
ATGGTAGCTGAGTGGAGGTTGTCTCGAGGTGTTGAAGAACAGACACAAGCTTTCTTTGAGGGCTTTAATG
AAATTCTTCCTCAGCAATATTTGCAATATTTTGATGCGAAGGAATTAGAGGTTCTTCTGTGTGGGATGCA
GGAGATTGATTTGAATGACTGGCAGAGACATGCCATCTACCGCCACTACACCAGAACAAGCAAGCAGATC
ATGTGGTTTTGGCAGTTTGTTAAAGAAATTGATAATGAGAAGAGGATGAGACTTCTGCAGTTTGTTACTG
GAACCTGCCGATTGCCAGTGGGAGGATTTGCTGACCTTATGGGGAGTAATGGACCACAGAAGTTCTGCAT
CGAAAAAGTTGGCAAAGAAAATTGGTTACCCAGAAGCCATACTTGTTTTAACCGCCTGGACCTCCCACCT
TACAAGAGCTATGAGCAACTGAAGGAAAAGCTGTTATTTGCCATTGAAGAAACTGAAGGATTTGGACAAG
AGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001243712
Insert Size 2595 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001243712.1, NP_001230641.1
RefSeq Size 5389 bp
RefSeq ORF 2595 bp
Locus ID 16396
UniProt ID Q8C863
Gene Summary Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:15358865, PubMed:16446428, PubMed:17592138, PubMed:18628966, PubMed:20392206, PubMed:25632008). It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation (By similarity). Involved in the control of inflammatory signaling pathways (By similarity). Is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways (By similarity). Promotes the association of the complex after TNF stimulation (By similarity). Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains (By similarity). This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1 (By similarity). Ubiquitinates RIPK2 by 'Lys-63'-linked conjugation and influences NOD2-dependent signal transduction pathways (By similarity). Regulates the transcriptional activity of several transcription factors involved in immune response (PubMed:15358865, PubMed:11828324). Ubiquitinates NFE2 by 'Lys-63' linkages and is implicated in the control of the development of hematopoietic lineages (By similarity). Mediates JUN ubiquitination and degradation (PubMed:15358865). Mediates JUNB ubiquitination and degradation (PubMed:11828324, PubMed:15358865). Critical regulator of type 2 helper T (Th2) cell cytokine production by inducing JUNB ubiquitination and degradation (PubMed:11828324). Involved in the negative regulation of MAVS-dependent cellular antiviral responses (By similarity). Ubiquitinates MAVS through 'Lys-48'-linked conjugation resulting in MAVS proteasomal degradation (By similarity). Following ligand stimulation, regulates sorting of Wnt receptor FZD4 to the degradative endocytic pathway probably by modulating PI42KA activity (By similarity). Ubiquitinates PI4K2A and negatively regulates its catalytic activity (By similarity). Ubiquitinates chemokine receptor CXCR4 and regulates sorting of CXCR4 to the degradative endocytic pathway following ligand stimulation by ubiquitinating endosomal sorting complex required for transport ESCRT-0 components HGS and STAM (By similarity). Targets DTX1 for lysosomal degradation and controls NOTCH1 degradation, in the absence of ligand, through 'Lys-29'-linked polyubiquitination (PubMed:18628966). Ubiquitinates SNX9 (By similarity). Ubiquitinates MAP3K7 through 'Lys-48'-linked conjugation (PubMed:25632008). Involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteasomal degradation of TXNIP (By similarity). Mediates the antiapoptotic activity of epidermal growth factor through the ubiquitination and proteasomal degradation of p15 BID (PubMed:20392206). Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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